INNOVEX BIOSCIENCES Uni-Trieve
使用方法： 只需40℃ 30min 或60-70℃ 15min
優點：
•不須煮沸, 也不用壓力鍋、微波爐加熱
•無citrate(檸檬酸)，因此不需要high pH buffer
•適用於所有primary antibody
•只需要water 或 dry bath
• background free
•適用於任何smear、animal和human組織，
ex brain, bone,marrow,etc
UNI-TRIEVE is a Mild -temperature Universal Retrieval Solution that retrieves for all antibodies, all human and all animal tissues at only 65-70 degrees celsius in 30 minutes using a simple water bath.
UNI-TRIEVE ; is Totally Background -Free and unlike citrate buffe, High pH buffers, pressure cookers or microwaves, no background or tissue damage or loss is observed with Uni-Trieve.
UNI-TRIEVE standardizes retrieval, it retrieves all tissues with a single solution.
UNI-TRIEVE's gentle temperature makes it an absolute must for retrieving for immunofluorescence, delicate tissues such as brain, bone marrow and animal tissues.
UNI-TRIEVE is Background-free and it is the retrieval of choice for Immunofluorescence (IF).
OVER-NIGHT Retrieval is now possible with UNI-TRIEVE: simply immerse sides in 40-45 degrees celcius Uni-Trieve using a water bath as the heat source. Next morning remove slides from the water bath , rinse and proceed with IHC or IF staining.
UNI-TRIEVE USER FRIENDLY PROTOCOL:
UNI-TRIEVE Unique Features
Order Uni-Trieve Online!
UNI-TRIEVE ; is Totally Background -Free and unlike citrate buffe, High pH buffers, pressure cookers or microwaves, no background or tissue damage or loss is observed with Uni-Trieve.
UNI-TRIEVE standardizes retrieval, it retrieves all tissues with a single solution.
UNI-TRIEVE's gentle temperature makes it an absolute must for retrieving for immunofluorescence, delicate tissues such as brain, bone marrow and animal tissues.
UNI-TRIEVE is Background-free and it is the retrieval of choice for Immunofluorescence (IF).
OVER-NIGHT Retrieval is now possible with UNI-TRIEVE: simply immerse sides in 40-45 degrees celcius Uni-Trieve using a water bath as the heat source. Next morning remove slides from the water bath , rinse and proceed with IHC or IF staining.
UNI-TRIEVE USER FRIENDLY PROTOCOL:
UNI-TRIEVE Unique Features
- Water bath Retrieval. NO microwaves. NO pressure cookers
- 30 minutes at 65-70C Water bath
- OVER-NIGHT retrieval at 40-45 degrees celsius
- pH-independent .One single solution retrieves all tissues for all antibodies.
- UNI-TRIEVE retrieves all animal and human tissues for all antibodies
- Total Retrieval time of 30 minutes at 65-70C for all membrane and cytoplasmic antibodies including Cytokeratins
- Total retrieval time of time of 45 minutes at 75-80 degrees celcius for nuclear antigen such as ki-67, ER, PR , etc.
- A must for cell line preps and delicate tissues such as brain , bone marrow and animal tissues.
- Background-Free
- Retrieval of choice for Immunofluorescence (IF)
- Can be used three times before needing to be discarded.
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IHC原理:是指在抗體上結合螢光或可呈色的化學物質，利用免疫學原理中抗原和抗體間專一性的結合反應，檢測細胞或組織中是否有目標抗原的存在。 此方式不只可以用來測知抗原的表現量也可觀察抗原所表現的位置。只要是能夠讓抗體結合的物質，也就是具有抗原性的物質包括蛋白質、核酸、多醣、病原體等都可偵測
IHC protocol:
1、Deparaffin-脫臘，65-75℃ 30min ,Xylene 10min, repeat X2
2、Dehydration-去除殘留切片上的Xylene
alcohol：100﹪→90﹪→80﹪→70﹪→Distill-Water→PBS
3、Optional-去除Endogenous peroxidase 活性，3﹪H2O2 10min
4、Antigen recovery(抗原修復)-石臘使Ag遮蔽，復原使Ag充分暴露
Citrate buffer or EDTA，Boiling（＞95℃）for 10 minutes ，Cool down
5、Block & quench-阻斷內源性的biotin作用，serum 10min
6、Primary Ab-1hr or O/N
7、Secondary Ab-1hr
8、Conjugate- (1)Peroxidase will catalyze the substrate 10min
(2)convert the chromogen to a color deposit
9、Substrate (DAB or AEC)- 5-10 min
10、Counterstain-5min
11、Dehydration-避免濃度劇烈的改變，導致組織內涵物的流失，造成樣本的傷害
alcohol：75﹪→85﹪→95﹪→100﹪→100﹪→Xylene
12、Mounting-mounting medium，5min
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