【抗體純化產品系列】移除溶液中BSA、Glycine、Tris、Azide等殘留分子，純化後溶液，可直接應用於抗體標誌套組 (lightning-link, innova biosciences) @ 太鼎生物科技/太鼎食安科技

抗體純化套組 Abselect kit

移除溶液中BSA、Glycine、Tris、Azide等殘留分子，純化後溶液，可直接應用於抗體標誌套組 (lightning-link, innova biosciences)

BSA、Gelatin、Glycine移除--àAbSelect 抗體-----純化系統

適合應用: 市售抗體。預移除溶液中BSA、Glycine、Tris、Azide等殘留分子

純化體積100ul至500ul

AbSelect抗體純化套組 (860-0010), 特別針對市售抗體保存液內, 含有BSA, Glycine. Tris. Azide等amino group干擾物質殘留。純化後的抗體, 可直接應用於抗體標誌試劑 (lightning-link一個步驟完成抗體標誌試驗)。

不須經由dialysis等純化步驟。抗體的體積可經由100ul至0.5ml，每次純化, 可以純化>500ug抗體量。

Ps. 蛋白質濃縮及buffer exchange之動作, 無法有效移除抗體內的小分子, 須經由Abselect 抗體純化系統來執行。

AbSelect Antibody Purification System

Commercially available antibodies often contain
substances (e.g. BSA, glycine, tris, azide) that interfere in labeling
reactions. The AbSelect™ Purification Kit quickly removes these contaminants.
It can also be used to purify antibodies from crude samples such as ascites
fluid or immune serum.

The antibody
to be purified or cleaned up ideally is in a volume of 100ul to 0.5ml. Up to
500µg of antibody can be purified in each run. The AbSelect method involves
capture of the antibody on protein A resin and the removal of unwanted
substances by a simple wash procedure. The purified product is then eluted and
neutralized.

The components
of the AbSelect kit are fully compatible with the Lightning-Link™ antibody labeling system
(available separately), which allows the purified antibody to be rapidly
conjugated with a wide variety of labels.

腹水、血清純化抗體à AbSelect 血清抗體------純化系統

適合應用: 腹水及血清產製之抗體。純化來自培養液、腹水及血清之抗體，移除殘留之amino group。純化後之抗體，可直接進行抗體標誌(lightning_link)、或奈米金標誌(innovaCoat)之試驗。

AbSelect Serum Antibody Purification System

Antibodies
generated from ascites fluid and serum are often supplied as crude
formulations. Both ascites fluid and serum contain many substances that
interfere with antibody labeling reactions. Successful labeling therefore
requires antibodies to be purified prior to labeling. The AbSelect Serum system
is a fast and simple method to purify antibodies from these types of media.

AbSelect Serum
is prepared by coupling highly purified protein A to agarose beads which can be
used to purify IgG fractions from ascites fluid and serum. The method involves
the capture of the antibody on the AbSelect Serum resin and the removal of
unwanted substances using a simple wash procedure. The purified product is then
eluted and neutralized.

The components
of the AbSelect Serum purification system are fully compatible with the
Lightning-Link™ antibody labeling kits (available separately),
which allows the purified antibody to be immediately labeled with an enzyme,
fluorescent protein or fluorescent dye, with a hands-on time of under 30
seconds.

腹水、血清純化抗體à AbSelect TCS組織培養液抗體------純化系統

適合應用: 融合瘤組織培養液(TCS)產製之抗體。純化來單株細胞產製之抗體，移除培養液中殘留之amino group。純化後之抗體，可直接進行抗體標誌(lightning_link)、或奈米金標誌(innovaCoat)之試驗。

AbSelect TCS Antibody Purification System

Antibodies are
often generated from hybridoma cell lines and supplied in tissue culture
supernatant (TCS). TCS itself contains many substances that interfere with
antibody labeling reactions. Successful labeling therefore requires antibodies
to be purified prior to labeling. The AbSelect TCS system is a fast and simple
method to purify these antibodies.

AbSelect TCS
is prepared by coupling highly purified protein A to agarose beads which can be
used to purify IgG fractions from hybridoma supernatants. The method involves
the capture of the antibody on the AbSelect TCS resin and the removal of
unwanted substances using a simple wash procedure. The purified product is then
eluted and neutralized.

The components
of the AbSelect TCS are fully compatible with the Lightning-Link™ antibody labeling kits (available separately),
which allows the purified antibody to be immediately labeled with an enzyme,
fluorescent protein or fluorescent dye, with a hands-on time of under 30
seconds.

抗體濃縮及Clean up系統

適合應用: 低濃度抗體之純化及濃縮。干擾酵素型HRP、Biotin、streptavidin及FITC螢光等之抗體標誌反應。移除溶液中殘留之amino group。純化後之抗體，可直接進行抗體標誌(lightning_link)、或奈米金標誌(innovaCoat)之試驗。

Antibody Concentration and Cleanup Kit

Antibodies are
sometimes only available at low concentrations and often contain low molecular
weight substances that interfere in labeling reactions with enzymes, biotin,
streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit
allows for the quick and easy concentration of antibodies and proteins. The kit
can also be used to reduce the concentration of many unwanted additives often
found in antibody formulations such as azide, glycine or tris.

The antibody
clean up kit method utilizes a simple spin column to easily and quickly remove
excess buffer from the antibody thereby providing a more concentrated antibody
solution. The clean up kit also allows the experimenter to perform a simple
buffer exchange to transfer the antibody into a more favorable conjugation
buffer.

DIY抗體標誌螢光(FITC,PE,Cy3,Cy5,Cy7 dye)

注意事項

請確認抗體的保存條件。傳統市售的抗體標誌套組，在使用前皆需dialysis純化，將抗體保存的物質移除。Lightning-link抗體標誌套組在大部份的保存條件下，不需要再dialysis的步驟，例如一般salts (e.g. NaCl)、sugars(e.g. sucrose)、及chelators (e.g. EDTA)不會影響標誌的活性。大部的市售的抗體保存在1%BSA、0.5%Azide及 0.5% Gelatin 輕微或幾乎不會影響標誌的活性。在少數情況下，若是您購買的抗體保存在amino acids(e.g. glycine)、blockers(e.g.
ethanolamine)及thiols(DTT, mercaptoethanol)情況下，記得純化您的抗體，(abselect 抗體純化套組) 避免抑制您的標誌活性了!