Fluoromount-G®防褪色劑是一種水性化合物，用於固定在螢光強度至關重要的顯微鏡應用中使用的載玻片。這種固定介質可提供用於密封載玻片製劑的半永久性密封，Fluoromount-G®Anti-Fade為水溶性的，因此可以通過將載玻片浸入PBS溶液中直至蓋玻片鬆動來去除蓋玻片。Fluoromount-G® Anti-Fade is an aqueous-based compound for mounting slides used in microscopy applications in which the preservation of fluorescence intensity is critical. This mounting medium provides a semi-permanent seal for storage of slide preparations and since Fluoromount-G® Anti-Fade is water-soluble, the coverslip may be removed by submerging the slide in a PBS solution until the coverslip is loosened.
Casas C, Isus L, Herrando-Grabulosa M, Mancuso FM, Borrás E, Sabidó E, et al. Network-based proteomic approaches reveal the neurodegenerative, neuroprotective and pain-related mechanisms involved after retrograde axonal damage. Sci Rep. 2015;5:9185. (IHC-FS, CY2, CY3, DAPI, NeuroTrace® 435/455 Blue)
Cheng C, Wang W, Huang C, Du P, Yang J, Tsaur M. Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons. J Comp Neurol. 2016;524:846-73. (IHC-FS, AF488, AF594, AF647, TSA™ CY3)
Cursio R, Miele C, Filippa N, Colosetti P, Auberger P, Van Obberghen E, et al. Tyrosine phosphorylation of insulin receptor substrates during ischemia/reperfusion-induced apoptosis in rat liver. Langenbecks Arch Surg. 2009;394:123-31. (IHC-FS, DAPI, Evans Blue, FLIVO®)
del Mar N, von Buttlar X, Yu AS, Guley NH, Reiner A, Honig MG. A novel closed-body model of spinal cord injury caused by high-pressure air blasts produces extensive axonal injury and motor impairments. Exp Neurol. 2015;271:53-71. (IHC-FS, AF488, AF594, AF647, EYFP)
Elsner RA, Hastey CJ, Olsen KJ, Baumgarth N. Suppression of long-lived humoral immunity following Borrelia burgdorferi infection. PLoS Pathog. 2015;11(17):e1004976. (IHC-FS, AF594, eFluor® 450, FITC)
Gaillard D, Xu M, Liu F, Millar SE, Barlow LA. β-catenin signaling biases multipotent lingual epithelial progenitors to differentiate and acquire specific taste cell fates. PLoS Genet. 2015;11(5):e1005208. (IHC-FS, ISH, TUNEL, AF488, AF546, BCIP/NBT, DRAQ5®, SYTOX™ Green, TMR Red, X-gal, YFP)
Herbig ME, Houdek P, Gorissen S, Zorn-Kruppa M, Wladykowski E, Volksdorf T, et al. A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin. Eur J Pharm Biopharm. 2015;95:99-109. (IHC-FS, IHC-PS, AF488, DAPI, MTT, Texas Red®)
Hilton BJ, Assinck P, Duncan GJ, Lu D, Lo S, Tetzlaff W. Dorsolateral funiculus lesioning of the mouse cervical spinal cord at C4 but not at C6 results in sustained forelimb motor deficits. J Neurotrauma. 2013;30:1070-83. (IHC-FS, CY3, DyLight® 405)
Imai DM, Feng S, Hodzic E, Barthold SW. Dynamics of connective-tissue localization during chronic Borrelia burgdorferi infection. Lab Invest. 2013;93:900-10. (IHC-FS, DAPI, NorthernLights™ 493)
Kim YU, Lim H, Jung HE, Wetsel RA, Chung Y. Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS One. 2015;10(3):e0120294. (IHC-FS, DyLight® 594, DyLight® 650, Image-iT® FX Signal Enhancer , Pacific Blue™)
Lang J, Ota T, Kelly M, Strauch P, Freed BM, Torres RM, et al. Receptor editing and genetic variability in human autoreactive B cells. J Exp Med. 2016;213:93-108. (IHC-FS, APC, DAPI, eFluor® 615)
Laver CR, Metcalfe AL, Szczygiel L, Yanai A, Sarunic MV, Gregory-Evans K. Bimodal in vivo imaging provides early assessment of stem-cell-based photoreceptor engraftment. Eye. 2015;29:681-90. (IHC-FS, ICC, NorthernLights™ 557, Qtracker® 655)
Lee CS, Hanna AD, Wang H, Dagnino-Acosta A, Joshi AD, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659. (IHC-FS, AF488, AF594, DAPI, Heochst 33342, PROTEOSTAT®)
Lee J, Kapur M, Li M, Choi M, Choi S, Kim H, et al. MFN1 deacetylation activates adaptive mitochondrial fusion and protects metabolically challenged mitochondria. J Cell Sci. 2014;127:4954-63. (IHC-FS, DAB, NBT)
Marsh SE, Abud EM, Lakatos A, Karimzadeh A, Yeung ST, Davtyan H, et al. The adaptive immune system restrains Alzheimer’s disease pathogenesis by modulating microglial function. Proc Natl Acad Sci USA. 2016;113:E1316-25. (IHC-FS, AF488, AF555, Amylo-Glo®)
McBride EG, Rubel EW, Wang Y. Afferent regulation of chicken auditory brainstem neurons: rapid changes in phosphorylation of elongation factor 2. J Comp Neurol. 2013;521:1165-83. (IHC-FS, AF488, AF568, NeuroTrace® 640/660 Deep-Red)
McClure S, Dudler L, Thorpe D, Hein WR. Analysis of cell division among subpopulations of lymphoid cells in sheep. I. Thymocytes. Immunology. 1988;65:393-9. (IHC-FS, ICC, DAB, Fast Red, Fast Blue, Hematoxylin)
Tran HP, Shin E, Saito K, Nguyen XT, Chung YH, Jeong JH, et al. Protective potential of IL-6 against trimethyltin-induced neurotoxicity in vivo. Free Radic Biol Med. 2012;52:1159-74. (IHC-FS, AF488, AF594, Fluoro-Jade®)
Michard E, Lima PT, Borges F, Silva AC, Portes MT, Carvalho JE, et al. Glutamate receptor-like genes form Ca2+ channels in pollen tubes and are regulated by pistil D-serine. Science. 2011;332:434-7. (IHC-FS, DRAQ5™, Marina Blue®)
Murphy S, Henry M, Meleady P, Zweyer M, Mundegar RR, Swandulla D, et al. Simultaneous pathoproteomic evaluation of the dystrophin-glycoprotein complex and secondary changes in the mdx-4cv mouse model of Duchenne muscular dystrophy. Biology. 2015;4:397-423. (IHC-FS, AF488, Hoechst 33342, Rhodamine Red™-X, Sudan Black B)
Piekarski N, Gross JB, Hanken J. Evolutionary innovation and conservation in the embryonic derivation of the vertebrate skull. Nat Commun. 2014;5:5661. (IHC-FS, AF488, Alizarin Red S, DAPI)
Qvartskhava N, Lang PA, Görg B, Pozdeev VI, Ortiz MP, Lang KS, et al. Hyperammonemia in gene-targeted mice lacking functional hepatic glutamine synthetase. Proc Natl Acad Sci USA. 2015;112:5521-6. (IHC-FS, CY3, Eosin, FITC, Hematoxylin, Hoechst 34580)
Sic H, Kraus H, Madl J, Flittner K, von Münchow AL, Pieper K, et al. Sphingosine-1-phosphate receptors control B-cell migration through signaling components associated with primary immunodeficiencies, chronic lymphocytic leukemia, and multiple sclerosis. J Allergy Clin Immunol. 2014;134:420-8. (IHC-FS, AF405, AF568, AF594, AmCyan, FITC)
Stone LM, Tan S, Tam PP, Finger TE. Analysis of cell lineage relationships in taste buds. J Neurosci. 2002;22:4522-9. (IHC-FS, CY5, DAPI, FITC, Lissamine™ Rhodamine B, PI)
Szabadfi K, Estrada C, Fernandez-Villalba E, Tarragon E, Setalo G Jr, Izura V, et al. Retinal aging in the diurnal Chilean rodent (Octodon degus): histological, ultrastructural and neurochemical alterations of the vertical information processing pathway. Front Cell Neurosci. 2015;9:126. (IHC-FS, AF488, AF568, CY3.5, DAPI, FITC)
Tan X, Xue Y, Ma T, Wang X, Li JJ, Lan L, et al. Partial eNOS deficiency causes spontaneous thrombotic cerebral infarction, amyloid angiopathy and cognitive impairment. Mol Neurodegener. 2015;10:24. (IHC-FS, AF488, AF555, AF594, DAPI, DyLight® 488, Fluoro-Emerald, Thioflavin S)
Wang H, Liu N, Zhang YP, Deng L, Lu Q, Shields CB, et al. Treadmill training induced lumbar motoneuron dendritic plasticity and behavior recovery in adult rats after a thoracic contusive spinal cord injury. Exp Neurol. 2015;271:368-78. (IHC-FS, Fast Blue, FITC, Fluoro-Gold, TRITC)
Watanabe K, Nishimura Y, Oka T, Nomoto T, Kon T, Shintou T, et al. In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives. BMC Neurosci. 2010;11:116. (IHC-FS, AF488, Coumarin)
Choi VW, Bigelow CE, McGee TL, Gujar AN, Li H, Hanks SM, et al. AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice. Mol Ther Methods Clin Dev. 2015;2:15022. (IHC-PS, AEC, Hematoxylin)
Han JJ, Mhatre AN, Wareing M, Pettis R, Gao W, Zufferey RN, et al. Transgene expression in the guinea pig cochlea mediated by a lentivirus-derived gene transfer vector. Hum Gene Ther. 1999;10:1867-73. (IHC-PS, ICC, AMCA, GFP, Texas Red®)
Berry R, Rodeheffer MS. Characterization of the adipocyte cellular lineage in vivo. Nat Cell Biol. 2013;15:302-8. (IHC-WM, AF647, BV421, dTomato, EGFP, HCS LipidTOX™ Deep Red)
Fry TL, Atwood T, Dunbar MR. Evaluation of rhodamine B as a biomarker for raccoons. Hum-Wildl Interact. 2010;4:275-82. (IHC-WM, Rhodamine B)
Jeffery E, Church CD, Holtrup B, Colman L, Rodeheffer MS. Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity. Nat Cell Biol. 2015;17:376-85. (IHC-WM, AF647, HCS LipidTOX™ Green)
McRae RL. Investigating metal homeostasis in mammalian cells using high resolution imaging techniques [thesis]. Atlanta (United States): Georgia Institute of Technology; 2010. (ER-Tracker™ Green, LysoTracker® Red)
Apaolaza PS, Delgado D, del Pozo-Rodríguez A, Gascón AR, Solinís MÁ. A novel gene therapy vector based on hyaluronic acid and solid lipid nanoparticles for ocular diseases. Int J Pharm. 2014;465:413-26. (ICC, AF488, Nile Red)
Aporta A, Arbues A, Aguilo JI, Monzon M, Badiola JJ, de Martino A, et al. Attenuated Mycobacterium tuberculosis SO2 vaccine candidate is unable to induce cell death. PloS One. 2012;7(9):e45213. (ICC, CellTracker™ Green, CellTracker™ Orange, Hoechst 33342)
Brady OA, Zhou X, Hu F. Regulated intramembrane proteolysis of the frontotemporal lobar degeneration risk factor, TMEM106B, by signal peptide peptidase-like 2a (SPPL2a). J Biol Chem. 2014;289:19670-80. (ICC, CF®488A, CF®568, CF®660C)
Callé A, Ugrinova I, Epstein AL, Bouvet P, Diaz J, Greco A. Nucleolin is required for an efficient herpes simplex virus type 1 infection. J Virol. 2008;82:4762-73. (ICC, AF633, FluoProbes® 488, FluoProbes® 547H)
Chemin K, Bohineust A, Dogniaux S, Tourret M, Guégan S, Miro F, et al. Cytokine secretion by CD4+ T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not microtubule organizing center polarity. J Immunol. 2012;189:2159-68. (ICC, AF546, AF647, CellTracker™ Blue)
Desouza CV, Hamel FG, Bidasee K, O'Connell K. Role of inflammation and insulin resistance in endothelial progenitor cell dysfunction. Diabetes. 2011;60:1286-94. (ICC, AF647, PE, PE/CY7)
Froger N, Orellana JA, Cohen-Salmon M, Ezan P, Amigou E, Sáez JC, et al. Cannabinoids prevent the opposite regulation of astroglial connexin43 hemichannels and gap junction channels induced by pro-inflammatory treatments. J Neurochem. 2009;111:1383-97. (ICC, AF488, AF555, Ethidium Bromide)
Hammond GR, Schiavo G, Irvine RF. Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P2. Biochem J. 2009;422:23-35. (ICC, DAPI, NBD C6-Ceramide)
Hangen E, De Zio D, Bordi M, Zhu C, Dessen P, Caffin F, et al. A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2. Cell Death Differ. 2010;17:1155-66. (ICC, AF488, dsRed, TO-PRO®-3)
Hasenberg A, Hasenberg M, Männ L, Neumann F, Borkenstein L, Stecher M, et al. Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes. Nat Methods. 2015;12:445-52. (ICC, APC, AF532, SYTOX™ Green)
Höbaus J, Tennakoon S, Heffeter P, Groeschel C, Aggarwal A, Hummel DM, et al. Impact of CYP24A1 overexpression on growth of colorectal tumour xenografts in mice fed with vitamin D and soy. Int J Cancer. 2016;138:440-50. (ICC, DAPI, DyLight® 550, GFP, MitoTracker® Red)
Holt BD, Dahl KN, Islam MF. Differential sub-cellular processing of single-wall carbon nanotubes via interfacial modifications. J Mater Chem B. 2015;3:6274-84. (ICC, DAPI, FM® 4-64, GFP, Hoechst 33342, Oregon Green® 488)
Holt BD, Roginskaya V, Van Houten B, Islam MF, Dahl KN. Dispersed single wall carbon nanotubes do not impact mitochondria structure or function, but technical issues during analysis could yield incorrect results. J Mater Chem B. 2017;5:369-74. (ICC, MitoTracker® Orange)
Hsu C, Poché RA, Saik JE, Ali S, Wang S, Yosef N, et al. Improved angiogenesis in response to localized delivery of macrophage-recruiting molecules. PLoS One. 2015;10(7):e0131643. (IHC-WM, ICC, AF546, AF647, DAPI, DsRed, EGFP, mCherry)
Jördens MS, Keitel V, Karababa A, Zemtsova I, Bronger H, Häussinger D, et al. Multidrug resistance-associated protein 4 expression in ammonia-treated cultured rat astrocytes and cerebral cortex of cirrhotic patients with hepatic encephalopathy. Glia. 2015;63:2092-105. (ICC, Hoechst 34580)
Jyoti K, Kaur K, Pandey RS, Jain UK, Chandra R, Madan J. Inhalable nanostructured lipid particles of 9-bromo-noscapine, a tubulin-binding cytotoxic agent: in vitro and in vivo studies. J Colloid Interface Sci. 2015;445:219-30. (ICC, DAPI, Rhodamine 6G)
Larsen ZH, Chander P, Joyner JA, Floruta CM, Demeter TL, Weick JP. Effects of ethanol on cellular composition and network excitability of human pluripotent stem cell-derived neurons. Alcohol Clin Exp Res. 2016;40:2339-50. (ICC, DAPI, DyLight® 488, DyLight® 550)
Lee H, Shin D, Song H, Yuk J, Lee Z, Lee S, et al. Nanoparticles up-regulate tumor necrosis factor-α and CXCL8 via reactive oxygen species and mitogen-activated protein kinase activation. Toxicol Appl Pharmacol. 2009;238:160-9. (ICC, CdSe-ZnS Quantum Dots, LysoTracker® Green)
Lerner N, Avissar S, Beit-Yannai E. Extracellular vesicles mediate signaling between the aqueous humor producing and draining cells in the ocular system. PLoS One. 2017;12(2):e0171153. (ICC, AF488, DAPI, DiD)
Ma Y, Weiss LM, Huang H. Inducible suicide vector systems for Trypanosoma cruzi. Microbes Infect. 2015;17:440-50. (ICC, TUNEL, DAPI, FITC, TACS® 2 TdT Blue)
McCormick PJ, Segarra M, Gasperini P, Gulino AV, Tosato G. Impaired recruitment of Grk6 and β-Arrestin 2 causes delayed internalization and desensitization of a WHIM syndrome-associated CXCR4 mutant receptor. PLoS One. 2009;4(12):e8102. (ICC, DyLight® 550, DyLight® 633)
Park S, Lee SJ, Chung H, Her S, Choi Y, Kim K, et al. Cellular uptake pathway and drug release characteristics of drug-encapsulated glycol chitosan nanoparticles in live cells. Microsc Res Tech. 2010;73:857-65. (ICC, CY5.5, Ce6, LysoTracker® Red)
Purdy AK, Campbell KS. SHP-2 expression negatively regulates NK cell function. J Immunol. 2009;183:7234-43. (ICC, AF350, AF488, AF635)
Puskás LG, Fehér LZ, Vizler C, Ayaydin F, Rásó E, Molnár E, et al. Polyunsaturated fatty acids synergize with lipid droplet binding thalidomide analogs to induce oxidative stress in cancer cells. Lipids Health Dis. 2010;9:56. (ICC, Oil Red O)
Riebeling C, Allegood JC, Wang E, Merrill AH Jr, Futerman AH. Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J Biol Chem. 2003;278:43452-9. (ICC, FITC, MitoTracker® Deep Red, TRITC)
Schaller T, Appel N, Koutsoudakis G, Kallis S, Lohmann V, Pietschmann T, et al. Analysis of hepatitis C virus superinfection exclusion by using novel fluorochrome gene-tagged viral genomes. J Virol. 2007;81:4591-603. (ICC, AF488, DAPI, GFP, RFP)
Tekle YI, Anderson OR, Lecky AF. Evidence of parasexual activity in "asexual amoebae" Cochliopodium spp. (Amoebozoa): extensive cellular and nuclear fusion. Protist. 2014;165:676-87. (ICC, AF488, AF594, CellMask™ Orange)
Thekkedath R, Koshkaryev A, Torchilin VP. Lysosome-targeted octadecyl-rhodamine B-liposomes enhance lysosomal accumulation of glucocerebrosidase in Gaucher's cells in vitro. Nanomedicine (Lond). 2013;8:1055-65. (ICC, DyLight® 350, LysoTracker® Red)
Gauthier LR, Granotier C, Hoffschir F, Etienne O, Ayouaz A, Desmaze C, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012;69:629-40. (FISH, CY3, DAPI, FITC)
Chabaud M, Heuzé ML, Bretou M, Vargas P, Maiuri P, Solanes P, et al. Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. Nat Commun. 2015;6:7526. (PLA, AF488)
Kruspe M, Thieme H, Guntinas-Lichius O, Irintchev A. Motoneuron regeneration accuracy and recovery of gait after femoral nerve injuries in rats. Neuroscience. 2014;280:73-87. (IHC, Fluoro-Emerald, Fluoro-Ruby®)
Lewis MD, Behrends J, Sá E Cunha C, Mendes AM, Lasitschka F, Sattler JM, et al. Chemical attenuation of Plasmodium in the liver modulates severe malaria disease progression. J Immunol. 2015;194:4860-70. (IHC, AF488, AF555, AF660, Hoechst 33258)
Lomeli N, Di K, Czerniawski J, Guzowski JF, Bota DA. Cisplatin-induced mitochondrial dysfunction is associated with impaired cognitive function in rats. Free Radic Biol Med. 2017;102:274-86. (IHC, FD Rapid GolgiStain™)
Phillips KG, Velasco CR, Li J, Kolatkar A, Luttgen M, Bethel K. et al. Optical quantification of cellular mass, volume, and density of circulating tumor cells identified in an ovarian cancer patient. Front Oncol. 2012;2:72. (Refractive Index)
Atto390 - is one of a new generation of fluorescentlabels with a coumarin structure. It has a strongabsorption at 388nm, a high fluorescence at 468nm(extinction coefficient 2.4 x104 cm-1M-1) and highquantum yield.
Click here for other labels available in the Lightning-Link® range.
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
Dylight® 350 - is a derivative of AMCA with a vibrantblue fluorescence that is brighter than AMCA and itsAlexa equivalent Alexa Fluor® 350. It has a strongabsorption at 354nm, high fluorescence at 432nm(extinction coefficient 1.5 x104 cm-1M-1) and highquantum yield.
Click here for other labels available in the Lightning-Link® range.
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
Unlike standard AMCA labeling procedures, where a large molar excess of AMCA is employed, the Lightning-Link AMCA kit combines a low molar ratio of AMCA:antibody and high labeling efficiency to eliminate the need for purification steps.
AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in-situ hybridisation. It has an excitation wavelength of 352nm and a maximal emission of 452nm.
Click here for other labels available in the Lightning-Link® range.
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
DyLight® 488 provides green fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 496nm, high fluorescence at 524nm (extinction coefficient 7.0 x104 cm-1M-1) and high quantum yield. Primary applications of DyLight® conjugates include immunohistochemistry, western blotting, ELISA or immunofluorescence microscopy. Click here for other labels available in the Lightning-Link® range.
Lightning-link抗體標誌V.S.保存液
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
R-Phycoerythrin (R-PE) is a fluorescent protein from the phycobiliprotein family, and is isolated from red algae. The absorbance spectrum of R-PE covers a broad wavelength range, which provides an advantage for multicolor immunofluorescent staining or flow cytometry as a number of excitation wavelengths can be used. R-PE is one of the most intensely fluorescent phycobiliproteins and has orange fluorescence. It is significantly brighter than conventional organic fluorophores. R-Phycoerythrin (R-PE) is a fluorescent protein from the phycobiliprotein family, present in red algae and cryptophytes. It has three maximal absorbance values of 498, 544 and 566nm (the optimal will depend on the application), and it has a strong emission peak at 580nm. RPE is closely related to B-Phycoerythrin (B-PE) and these are the most intense fluorescent phycobiliproteins providing an orange fluorescence.
RPE conjugates are commonly used for immunofluorescence and flow cytometry and are ideal for multiple labeling with other fluorochromes.
Click here to view other fluorescent proteins and dyes available in the Lightning-Link® range.
Lightning-link抗體標誌V.S.保存液:
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
Fluorescein is one of the most popular fluorescent reagents used in biological research because of its water solubility, intense fluorescence and high absorptivity. It has a peak excitation occurring at 498nm and peak emission of 532nm. Fluorescein conjugates are very popular for flow cytometry and immunofluorescence and are also used for immunohistochemistry. Click herefor other labels available in the Lightning-Link® range.
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
近紅外光DyLight® 755 DyLight® 755 provides far red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 756nm, high fluorescence at 794nm (extinction coefficient 2.2 x105 cm-1M-1) and high quantum yield.
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
DyLight® 800 provides near infrared fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 776nm, high fluorescence at 798nm (extinction coefficient 2.7 x105 cm-1M-1) and high quantum yield.
Applicable to Western Blotting, ELISA, Immunohistochemistry, Immunofluorescence, and FACS analysis
From 10µg up to 5mg
Lightning-link抗體標誌V.S.保存液:
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
近紅外光DyLight® 800 provides near infrared fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 776nm, high fluorescence at 798nm (extinction coefficient 2.7 x105 cm-1M-1) and high quantum yield.
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
FluoProbes®647H is one of a new generation of fluorescent labels designed for labelling biomolecules. It has a strong absorption at 653nm, high fluorescence at 674nm (extinction coefficient 2.5 x105 cm-1M-1) and high quantum yield. FluoProbes®647H can be used as a substitute dye for Cy5, BODIPY 650/665, DY-650 and Alexa Fluor 647.
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.
The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.
The Lightning-Link® range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link® conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.
Biotin Label
The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. The biotin in the kit has an extended linker to facilitate molecular interactions.
Lightning-Link® Biotin has been optimised for two separate applications. Type A is intended for assays in which a streptavidin-labeled detection reagent will be used, whilst Type B is optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
The technology is fully scalable (μg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.
Manufacturing
The technology is fully scalable (μg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.
Type A - 3 reactions (up to 20ug scale) (704-0030)
Type A - 3 reactions (up to 200ug scale) (704-0010)
Type A - 1 reaction (up to 2mg scale) (704-0015)
Type B - 3 reactions (up to 20ug scale) (715-0030)
Type B - 3 reactions (up to 200ug scale) (715-0010)
Type B - 1 reaction (up to 2mg scale) (715-0015)
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Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.
The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.
The Lightning-Link® range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link® conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.
Horseradish Peroxidase (HRP) Label
Horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues which can be conjugated to labeled biomolecules using Lightning-Link®. The enzyme label can be visualized by chromogenic reactions; for example diaminobenzidine (DAB) in the presence of hydrogen peroxide (H202) is converted in to a water insoluble brown pigment. Other substrates which can be used to measure horseradish peroxidase activity include ABTS, TMB and TMBUS.
The technology is fully scalable (μg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.
3 x 10ug HRP (up to 40ug Ab) (701-0030)
1 x 100ug HRP (up to 400ug Ab) (701-0010)
3 x 100ug HRP (each up to 400ug Ab) (701-0000)
1 x 1mg HRP (up to 4mg Ab) (701-0002)
5 x 1mg HRP (each up to 4mg Ab) (701-0003)
1 x 5mg HRP (up to 20mg Ab) (701-0004)
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