太鼎生物科技/太鼎食安科技

Innova biosciences AbSelect TCS Hybridoma單株抗體純化系統。高效能純化tissue culture supernatant (TCS)

【單株抗體經由融合瘤細胞株tissue culture supernatant (TCS)產製】

TCS本身包括許多雜質會干擾抗體後續下游分析，例如抗體標誌試驗。成功地抗體標誌要求單株抗體純化過程中須經由純化的步驟，innova biosciences【AbSelect TCS系統】提供快速且簡單純化單株抗體的方法。其原理為應用高效能且高純度Protein A結合於Agarose beads上，純化Hybridoma 上清液的IgG片段。 AbSelect TCS resin純化最多的抗體，經由簡單的wash步驟將非目標蛋白移除，再經由eluted步驟將單株抗體回收。

【AbSelect TCS系統特點】

·      All components and buffers supplied

·      Purify antibody from 10 to 50mls of hybridoma TCS

AbSelect TCS (1 purification : 10 to 50ml tissue culture supernatant) (862-0030)

bSelect TCS (3 purifications : 10 to 50ml tissue culture supernatant) (862-0500)

Hybridoma cell linesAntibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies.

AbSelect TCS is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from hybridoma supernatants.  The method involves the capture of the antibody on the AbSelect TCS resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.

The components of the AbSelect TCS are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.

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原廠連結: http://www.innovabiosciences.com/

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