~【NEW!!活動快報】~ 全景玻片影像掃描器,精準對焦與掃描,染色結果從此數位化
MoticEasyScan 全景玻片影像掃描器,3D多層次掃描,快速對焦精準掃描為數位玻片。提供最佳解析度0.25um/pixel ;6片式片夾,walk-away設計。搭配簡易操作軟體,優質影像數位永久保存。歡迎來電洽詢(02)86609496許虹宜

目前分類:【藥物篩選、癌症行為、抑制劑】 (93)

瀏覽方式: 標題列表 簡短摘要

雷帕黴菌(Rapamycin)Romidepsin (FK228, depsipeptide) | Daptomycin G-418 (80 S 核醣體延伸抑制劑) 

鹽酸二甲雙胍(Metformin HCl)  | CAS#1115-70-4 APExBIO  貨號B1970 (10mM/10g/50g) 

Anti-diabetic drug

鹽酸二甲雙胍(Metformin HCl) 是治療 2 型糖尿病(type 2 diabetes)最有效和廣泛使用的治療藥物之一。它選擇性地降低肝臟糖異生(hepatic gluconeogenesis)而不增加胰島素(insulin)的產生,從而導致體重增加(weight gain)或低血糖(hypoglycemia)。 [1]AMPK(5'AMP 活化蛋白激酶)充當代謝主開關(metabolic master switch),調節多個細胞內系統,包括的細胞葡萄糖攝取(cellular uptake of glucose)、脂肪酸的 β 氧化(-oxidation)以及 GLUT4(glucose transporter 4, 葡萄糖轉運蛋白 4)和粒線體(mitochondria)的生物合成。在肝細胞(hepatocytes)中,AMPK 被二甲雙胍(metformin)激活,隨後 ACC(acetyl-CoA carboxylase, 乙醯輔酶 A 羧化酶)活性降低,誘導脂肪酸氧化(fatty acid oxidization)並抑制脂肪生成酶(lipogenic enzyme expression)表達。 [2] Metformin還抑制 mGPD(mitochondrial lycerophosphate dehydrogenase, 線粒體甘油磷酸脫氫酶),一種氧化還原穿梭酶(redox shuttle enzyme),導致肝細胞氧化還原狀態改變(hepatocellular redox state)、乳酸(lactate)轉化減少和肝醣異生(hepatic gluconeogenesis)減少。 [1]在用metformin治療的大鼠中,SEREP-1 mRNA/蛋白的肝臟表達和 ACC 活性降低。 [2] 在二甲雙胍治療的小鼠中,肝臟中的 LKB1 對於metformin降低血糖的能力至關重要 [3]。在大鼠肝臟 mGOD 的 ASO(反義寡核苷酸)敲低中,表型與慢性二甲雙胍治療相似。它消除了 mefromin 誘導的細胞溶質氧化還原狀態、血漿葡萄糖濃度的降低和 EGP 抑制。 [1]

鹽酸二甲雙胍(Metformin HCl)  CAS#1115-70-4 APExBIO  貨號B1970.jpg

3 鹽酸二甲雙胍(Metformin HCl) CAS#1115-70-4 APExBIO  貨號B1970.jpg

 

 

 

Physical Appearance A solid
Storage Store at -20°C
M.Wt 165.62
Cas No. 1115-70-4
Formula C4H12ClN5
Solubility insoluble in EtOH; ≥30.7 mg/mL in H2O; ≥8.3 mg/mL in DMSO
Chemical Name 3-(diaminomethylidene)-1,1-dimethylguanidine;hydrochloride
SDF Download SDF
Canonical SMILES CN(C)C(=N)N=C(N)N.Cl
Shipping Condition Ship with blue ice, or upon other requests.
General tips For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

2 鹽酸二甲雙胍(Metformin HCl) CAS#1115-70-4 APExBIO  貨號B1970.jpg

 

https://www.apexbt.com/

APExBIO台灣代理商

APExBIO提供優質的勝肽和生物分析試劑,廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。

DNA Damage/DNA Repair CAS# #貨號 應用
SCR7 CAS#1533426-72-0 貨號A8705 DNA ligase IV inhibitor
RHPS4n CAS#390362-78-4 貨號B6186 Telomerase inhibitor
AZD6738 CAS#1352226-88-0 貨號B6007 ATR inhibitor

ORY-1001

 

CAS#1431326-61-2 貨號B5882 Selective inhibitor of KDM1A.
RGFP966 CAS#1357389-11-7;1396841-57-8 貨號A8803 Specific HDAC3 inhibitor
Oxaliplatin CAS#61825-94-3 貨號A8648 Antitumor agent
(S)-Crizotinib CAS#877399-52-5;1374356-45-2

貨號A8802

 

Potent MTH1 inhibitor
Tubastatin A CAS#1252003-15-8 貨號A4101 HDAC6 inhibitor,potent and selective

 

 

APExBIO產品線 品項 CAS# #貨號
Proteases Z-VAD-FMK | CAS# 187389-52-2 APExBIO  CAS# 187389-52-2 貨號 A1902
Proteinase K 蛋白酶 K (Proteinase K) | CAS#39450-01-6 APExBIO  貨號K1037 CAS#39450-01-6 貨號K1037
品項 CAS# #貨號 應用
Romidepsin (FK228, depsipeptide)

CAS#128517-07-7

 

貨號A8173 HDAC1/HDAC2,強效和選擇性抑制劑
Pirarubicin CAS#72496-41-4 貨號B2295  anthracycline anti-neoplastic doxorubicin
Tunicamycin CAS# 15663-27-1

貨號A8321

 

高效、廣譜的化療藥物
Azithromycin CAS#83905-01-5  貨號A2213 Hsp90 inhibitor
Bleomycin Sulfate

CAS# 9041-93-4

 

貨號B1398 Antibiotic by inhibiting protein synthesis
Nystatin (Fungicidin) CAS#1400-61-9 貨號B1993 antifungal antibiotic
Rapamycin CAS#53123-88-9 貨號A8167 Original antifungal antibiotic
Daptomycin CAS#103060-53-3 貨號A1206 Calcium-dependent antibiotic
品項 CAS# #貨號 應用
Z-VAD-FMK   CAS# 187389-52-2 貨號 A1902

Proteases

ICE-like proteases

TAPI-1   CAS#171235-71-5 貨號 B4686

Proteases

TACE/ADAM17 inhibitor Product Citation+3

Talabostat mesylate CAS# 150080-09-4 貨號B3941

Proteases

DPP4 二肽基肽酶特異性抑制劑

Z-IETD-FMK CAS# 210344-98-2 貨號B3232 inhibitor of caspase 8
Cisplatin CAS# 15663-27-1 貨號A8321 高效、廣譜的化療藥物
17-DMAG (Alvespimycin) HCl CAS#467214-21-7  貨號A2213 Hsp90 inhibitor
CA-074 Me  CAS# 147859-80-1 貨號A8239 Cathepsin B inhibitor
VX-765 CAS# 273404-37-8 貨號A8238 Caspase-1 inhibitor
文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

雷帕黴菌(Rapamycin)Romidepsin (FK228, depsipeptide) | Daptomycin G-418 (80 S 核醣體延伸抑制劑)

Vancomycin hydrochloride is a hydrochloride of vancomycin that is a narrow-spectrum glycopeptide antibacterial agent.

Vancomycin hydrochloride

Vancomycin hydrochloride | CAS#1404-93-9 APExBIO  貨號B1223

Physical Appearance A solid
Storage Store at -20°C
M.Wt 1485.72
Cas No. 1404-93-9
Formula C66H76Cl3N9O24
Solubility ≥55.8 mg/mL in DMSO with gentle warming; insoluble in EtOH; ≥22.15 mg/mL in H2O
SDF Download SDF
Shipping Condition Ship with blue ice, or upon other requests.
General tips For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

 

 

Animal models C57BL/6 mice challenged with Clostridium difficile (C. difficile) strain VPI 10463
Dosage form

20 mg/kg

Once daily by oral gavage for 5 days

Applications Vancomycin treatment of infected mice was associated with improved clinical, diarrhea, and histopathology scores and survival during treatment. However, after discontinuation of the drug, clinical scores and histopathology were worse in treated mice than in untreated infected controls. Decreasing the duration of vancomycin treatment from 5 days to 1 day decreased recurrence and deaths in C. difficile infection.
Note The technical data provided above is for reference only.
 

References:

1. Warren CA, van Opstal EJ, Riggins MS, et al. Vancomycin treatment's association with delayed intestinal tissue injury, clostridial overgrowth, and recurrence of Clostridium difficile infection in mice. Antimicrobial Agents and Chemotherapy, 2013, 57(2): 689-696.

 

APExBIO台灣代理商

APExBIO提供優質的胜肽和生物分析試劑,廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

雷帕黴菌(RapamycinRomidepsin (FK228, depsipeptide) | Daptomycin G-418 (80 S 核醣體延伸抑制劑) 

2-NBDG | CAS#186689-07-6 APExBIO  貨號B6035

APExBIO盒子樣品1-300x246.jpg

fluorescent glucose analog for visualizing glucose uptake into living cells.

2-NBDG 是一種螢光標記的 2-脫氧葡萄醣(2-deoxy-glucose)類似物(analog),可用作評估細胞葡萄糖代謝(cellular glucose metabolism)的示蹤劑(tracer)。葡萄糖(Glucose)是維持組織中細胞活動(cell activities)恆定狀態(homeostasis)的必要能量來源。糖代謝(Glucose metabolism)是許多疾病的重要靶標,並隨著病理狀況(pathological condition)而變化,因此,對糖代謝(glucose metabolism)的評估可以成為疾病進展的重要指標。

2-NBDG可用於多種體外細胞 (10uM),如HepG2人肝癌細胞(HepG2 human hepatocarcinoma cells)L6大鼠骨骼肌細胞(L6 rat skeletal muscle cells)MCF-7乳腺癌上皮細胞(MCF-7 breast cancer epithelial cells)和星形膠質細胞(astrocytes),也用於疾病模型(disease models)、癲癇大鼠(epilepsy rat)、高血糖(hyperglycemia)、糖尿病(diabetes)或小鼠癌症異種移植模型(mouse xenograft model of cancer)

2-NBDG 通過葡萄糖轉運(glucose transporters)蛋白進入細胞,隨後被己糖激酶(hexokinase)磷酸化(phosphorylated)並被困在細胞內。可以對細胞產生的螢光進行流式細胞術檢測(Flow cytometric detection),以檢查活細胞對 2-NBDG 的攝取(2-NBDG uptake),並可以使用螢光微孔板測定法(fluorescence microplate assay)測量轉運的 2-NBDG 的細胞內濃度。它也可以簡單地用螢光成像顯微鏡(fluorescence imaging microscopy) CCD 相機檢測。

http://biopioneer.com.tw/wp-content/uploads/downloads/2022/12/2-NBDG-CAS186689-07-6-APExBIO-貨號B6035.pdf 

7. 2-NBDG  CAS#186689-07-6 APExBIO  貨號B6035.jpg

5 2-NBDG CAS#186689-07-6 APExBIO  貨號B6035.jpg

6. 2-NBDG CAS#186689-07-6 APExBIO  貨號B6035.jpg

Product Citation

Physical Appearance

A crystalline solid

Storage

Store at -20°C

M.Wt

342.26

Cas No.

186689-07-6

Formula

C12H14N4O8

Solubility

insoluble in DMSO; ≥17.1 mg/mL in H2O with ultrasonic; ≥2.93 mg/mL in EtOH with gentle warming and ultrasonic

Chemical Name

(3R,4R,5S,6R)-6-(hydroxymethyl)-3-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)tetrahydro-2H-pyran-2,4,5-triol

SDF

Download SDF

Canonical SMILES

OC[C@](O1)([H])[C@](O)([H])[C@@](O)([H])[C@](NC2=CC=C(N(=O)=O)C3=NON=C23)([H])C1([H])O

Shipping Condition

Ship with blue ice, or upon other requests.

General tips

For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

 

APExBIO台灣代理商

APExBIO提供優質的勝肽和生物分析試劑,廣泛產品線如ProteaseChromatin/Epigenetics表觀遺傳學、MAPK SignalDNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kitDrug Screening panelBioactive peptidesGrowth factorsBiotinylation Reagentstag peptidesamino AcidsGrowth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。

 

APExBIO產品線

品項

CAS#

#貨號

Proteases

Z-VAD-FMK | CAS# 187389-52-2 APExBIO 

CAS# 187389-52-2

貨號 A1902

Proteinase K

蛋白酶 K (Proteinase K) | CAS#39450-01-6 APExBIO  貨號K1037

CAS#39450-01-6

貨號K1037

品項

CAS#

#貨號

應用

Romidepsin (FK228, depsipeptide)

CAS#128517-07-7

 

貨號A8173

HDAC1/HDAC2,強效和選擇性抑制劑

Pirarubicin

CAS#72496-41-4

貨號B2295

 anthracycline anti-neoplastic doxorubicin

Tunicamycin

CAS# 15663-27-1

貨號A8321

 

高效、廣譜的化療藥物

Azithromycin

CAS#83905-01-5

 貨號A2213

Hsp90 inhibitor

Bleomycin Sulfate

CAS# 9041-93-4

 

貨號B1398

Antibiotic by inhibiting protein synthesis

Nystatin (Fungicidin)

CAS#1400-61-9

貨號B1993

antifungal antibiotic

Rapamycin

CAS#53123-88-9

貨號A8167

Original antifungal antibiotic

Daptomycin

CAS#103060-53-3

貨號A1206

Calcium-dependent antibiotic

品項

CAS#

#貨號

應用

Z-VAD-FMK  

CAS# 187389-52-2

貨號 A1902

Proteases

ICE-like proteases

TAPI-1  

CAS#171235-71-5

貨號 B4686

Proteases

TACE/ADAM17 inhibitor Product Citation+3

Talabostat mesylate

CAS# 150080-09-4

貨號B3941

Proteases

DPP4 二肽基肽酶特異性抑制劑

Z-IETD-FMK

CAS# 210344-98-2

貨號B3232

inhibitor of caspase 8

Cisplatin

CAS# 15663-27-1

貨號A8321

高效、廣譜的化療藥物

17-DMAG (Alvespimycin) HCl

CAS#467214-21-7

 貨號A2213

Hsp90 inhibitor

CA-074 Me 

CAS# 147859-80-1

貨號A8239

Cathepsin B inhibitor

VX-765

CAS# 273404-37-8

貨號A8238

Caspase-1 inhibitor

 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

雷帕黴菌(Rapamycin)Romidepsin (FK228, depsipeptide) | Daptomycin G-418 (80 S 核醣體延伸抑制劑)

APEXBIO.jpg

Natamycin | CAS#7681-93-8抗真菌大環內酯多烯Antifungal macrolide polyene APExBIO貨號A5786

Natamycin CAS#7681-93-8抗真菌大環內酯多烯Antifungal macrolide polyene APExBIO貨號A5786.png

Pimaricin, Streptomyces chattanoogensis 是一種抗真菌大環內酯多烯(antifungal macrolide polyene),可與細胞膜甾醇(membrane sterols)結合。大環內酯類(Macrolides)屬於一組與核醣體 50S 亞基(50S ribosomal subunit)結合併抑制蛋白質合成的抗生素。

操作方法.jpg

Physical Appearance A solid
Storage Store at -20°C
M.Wt 665.73
Cas No. 7681-93-8
Formula C33H47NO13
Solubility insoluble in EtOH; insoluble in H2O; ≥16.43 mg/mL in DMSO with gentle warming
SDF Download SDF
Canonical SMILES O[C@](C1)(C[C@H](C[C@H]2O[C@@H]2/C=C\C(O[C@H](C)C/C=C\C=C/C=C\C=C/[C@@H]3O[C@@H]([C@H]([C@H]4N)O)O[C@H](C)[C@H]4O)=O)O)O[C@](C3)([H])[C@H](C(O)=O)[C@H]1O
Shipping Condition Ship with blue ice, or upon other requests.
General tips For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

APExBIO台灣代理商

APExBIO提供優質的胜肽和生物分析試劑,廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。

APExBIO產品線 品項 CAS# #貨號
Proteases Z-VAD-FMK | CAS# 187389-52-2 APExBIO  CAS# 187389-52-2 貨號 A1902
Proteinase K 蛋白酶 K (Proteinase K) | CAS#39450-01-6 APExBIO  貨號K1037 CAS#39450-01-6 貨號K1037
品項 CAS# #貨號 應用
Romidepsin (FK228, depsipeptide)

CAS#128517-07-7

 

貨號A8173 HDAC1/HDAC2,強效和選擇性抑制劑
Pirarubicin CAS#72496-41-4 貨號B2295  anthracycline anti-neoplastic doxorubicin
Tunicamycin CAS# 15663-27-1

貨號A8321

 

高效、廣譜的化療藥物
Azithromycin CAS#83905-01-5  貨號A2213 Hsp90 inhibitor
Bleomycin Sulfate

CAS# 9041-93-4

 

貨號B1398 Antibiotic by inhibiting protein synthesis
Nystatin (Fungicidin) CAS#1400-61-9 貨號B1993 antifungal antibiotic
Rapamycin CAS#53123-88-9 貨號A8167 Original antifungal antibiotic
Daptomycin CAS#103060-53-3 貨號A1206 Calcium-dependent antibiotic
品項 CAS# #貨號 應用
Z-VAD-FMK   CAS# 187389-52-2 貨號 A1902

Proteases

ICE-like proteases

TAPI-1   CAS#171235-71-5 貨號 B4686

Proteases

TACE/ADAM17 inhibitor Product Citation+3

Talabostat mesylate CAS# 150080-09-4 貨號B3941

Proteases

DPP4 二肽基肽酶特異性抑制劑

Z-IETD-FMK CAS# 210344-98-2 貨號B3232 inhibitor of caspase 8
Cisplatin CAS# 15663-27-1 貨號A8321 高效、廣譜的化療藥物
17-DMAG (Alvespimycin) HCl CAS#467214-21-7  貨號A2213 Hsp90 inhibitor
CA-074 Me  CAS# 147859-80-1 貨號A8239 Cathepsin B inhibitor
VX-765 CAS# 273404-37-8 貨號A8238 Caspase-1 inhibitor

原廠型錄

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

3M油質老化測試紙 | 貨號1005  

 

- 油質測試,一紙即知 -

3M油脂試紙-285x300.jpg

利用酸鹼試紙來測試油脂的酸價,其原理是因為油脂是由脂肪酸與甘油組成,經高溫烹煮後會逐漸裂解出游離脂肪酸而呈現酸性,游離出的脂肪酸越多酸價就越高。3M油脂老化測試紙主要檢測炸油在劣化過程中,濃度漸升的酸價AV (mg KOH/g)。3M炸油監測法使用具有四道色帶的試紙,只要炸油中的酸價AV升高,色帶顏色即從藍色轉變成黃色。只要將試紙浸入炸油內再拿出,數秒後,色帶就會開始變色,並且顯示炸油的劣化程度。超過設定標準,即須立刻進行換油。

搶購.jpg

使用流程:

截圖-2022-06-21-下午4.34.02.png

浸-將四個藍色區間全部浸在熱的(163°C~204°C)炸油中3秒。

截圖-2022-06-21-下午4.35.03.png

等-自油中取出後,等候30秒使其呈色。

截圖-2022-06-21-下午4.35.59.png

算-計算有多少區間由藍變黃。

截圖-2022-06-21-下午4.36.58.png

丟-丟棄油質老化測試紙(只能使用一次)。

結果判讀:

酸價超過2.0 mg KOH/g 即1格變黃就必須換油​

截圖-2022-06-21-下午4.41.51.png

保存:

保存在低溫(-4 ~ 4 °C)乾燥的地方。

注意事項:

請勿將產品放置於非建議之保存環境超過36小時,否則產品將會有變質之風險。

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

5(6)-CFDA, SE |  5-(and-6)-Carboxyfluorescein diacetate 貨號BMD0066

Fig. 5(6)-CFDA, SE structure formula

5(6)-CFDA, SE(全名:5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester,也稱為 CFSE)是一種有用的螢光示踪劑(fluorescent tracer),可以被動擴散到細胞中並共價標記細胞內蛋白質(intracellular proteins),從而導致長期細胞標記。該試劑本身是無色無螢光的,但一旦被細胞內酯酶(intracellular esterases)水解,就會變成亮綠色螢光。

abbkine02-285x300.png

Product name 5(6)-CFDA, SE
CAS Number 150347-59-4
Molecular Formula C29H19NO11
Alternative 5(6)-CFDA, SE,5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester; CFSE; BMD00066

Product Properties

Formulation White solid or Pale yellow solid
Features & Benefits • λEX/λEm: 494/521。(pH=7)
Molecular weight 557.47
Usage notes • Soluble in DMSO
Storage instructions Store at -20°C and protect from light. Product is stable for at least 12 months from date of receipt if stored as recommended.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

搶購.jpg

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 | Abbkine 細胞膜染色 | 活細胞Lysosome染色

Product Name Catalog No
TraKine™ Cell Plasma Membrane Staining Kit (Green Fluorescence) KTC4001
TraKine™ Cell Plasma Membrane Staining Kit (Orange Fluorescence) KTC4002
TraKine™ Mitochondrion Staining Kit (Green Fluorescence) KTC4003
TraKine™ Mitochondrion Staining Kit (Orange Fluorescence) KTC4004
TraKine™ Mitochondrion and Nuclear Staining Kit KTC4005
TraKine™ F-actin Staining Kit (Green Fluorescence) KTC4008
TraKine™ F-actin Staining Kit (Orange Fluorescence) KTC4009
TraKine™ Pro Live-cell Tubulin-traker kit (Green Fluorescence) KTC4100
TraKine™ Pro Live-cell Lyso-traker kit (Red Fluorescence) KTC4210
TraKine™ Pro Live-cell Tubulin-traker kit (Red Fluorescence) KTC4110
TraKine™ Pro Live-cell Lyso-traker kit (Green Fluorescence) KTC4200
Cy3 BMD0050
Cy5 BMD0051
Ethidium Homodimer-1 (EthD-1) BMD0060
Hoechst 33258 BMD0061
Hoechst 33342 BMD0062
DAPI BMD0063
Calcein AM BMD0064
5(6)-CFDA, SE BMD0066
DiI (DiIC18(3)) BMD0071
DiO (DiOC18(3)) BMD0072
DiD (DiIC18(5)) BMD0073
DiR (DiIC18(7)) BMD0074
AbFluor™ 488-Phalloidin BMD0082
AbFluor™ 555-Phalloidin BMD0083
AbFluor™ 594-Phalloidin BMD0084
文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

雷帕黴菌(Rapamycin)Romidepsin (FK228, depsipeptide) | Daptomycin G-418 (80 S 核醣體延伸抑制劑) 

Bay 11-7821 (BAY 11-7082) | CAS# 19542-67-7 APExBIO  貨號A4210

Product Citation+7

IKK 抑制劑

 

Bay 11-7821 (Bay 11-7082) 是一種 IKK 抑制劑,IC50 值為 10μM [1]。Bay 11-7821 抑制 TNFα 誘導的 IκB-α 磷酸化,從而抑制 NF-κB。這隨後導致抑制 TNFα 誘導的 E-選擇素(E-selectin)、VCAM-1 和 ICAM-1 的表面表達。據報導,Bay 11-7821 不會抑制整個 TNFα 誘導的磷酸化(phosphorylation),而是選擇性地抑制 IκB-α 的磷酸化 [1]。實驗表明,Bay 11-7821 在有效 IKK 抑制濃度下具有劇毒。它可以在MM細胞中誘導細胞壞死。此外,Bay 11-7821 通過誘導 B 細胞淋巴瘤和白血病 T 細胞死亡被證明具有抗炎能力。據報導,它還能抑制巨噬細胞中的 NALP3 炎性體 [2, 3]。

Bay 11-7821 (BAY 11-7082) CAS# 19542-67-7 APExBIO  貨號A4210.jpg

2 Bay 11-7821 (BAY 11-7082) CAS# 19542-67-7 APExBIO  貨號A4210.jpg

 

 

 

 

  1. Can Liu, Qiu Peng, et al. " Deficiency of Lactoferrin aggravates lipopolysaccharide-induced acute inflammation via recruitment macrophage in mice." Biometals. 2022 Jun 1;1-14. PMID: 35650365
  2. Mingze Ma, Fengxian Shi, et al. "TGF-β promote epithelial-mesenchymal transition via NF-κB/NOX4/ROS signal pathway in lung cancer cells." Mol Biol Rep. 2021 Mar;48(3):2365-2375.PMID: 33792826
  3. Liu Hui-heng, Wang Jun-sheng, et al. "LPS induced PCT production via TLR-4/NF-кB passway: it is the difference of G-/G+ bacteremia rats." Cytokine. 2020 Oct 7;137:155317.PMID: 33039977
  4. Ma L, Jiang M, et al. "Cigarette and IL-17A synergistically induce bronchial epithelial-mesenchymal transition via activating IL-17R/NF-κB signaling." BMC Pulm Med. 2020;20(1):26. Published 2020 Jan 30. PMID: 32000730
  5. Zhang X, Yan K, et al. "Cyclooxygenase 2 promotes proliferation and invasion in ovarian cancer cells via the PGE2/NF-κB pathway." Cell Transplant. 2019 Dec 10:963689719890597.PMID: 31822119
  6. Wang Z, Chen J, et al. "cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity." J Clin Invest. 2019 Aug 13;130:4850-4862.PMID: 31408442
  7. MXinwei Feng, Junfeng Lu, et al. "Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells." Front. Cell. Infect. Microbiol. 19 June 2018.

3. Bay 11-7821 (BAY 11-7082)  CAS# 19542-67-7 APExBIO  貨號A4210.jpg

Storage Store at -20°C
M.Wt 207.25
Cas No. 19542-67-7
Formula C10H9NO2S
Solubility insoluble in H2O; ≥64 mg/mL in DMSO; ≥10.64 mg/mL in EtOH with gentle warming and ultrasonic
Chemical Name (E)-3-(4-methylphenyl)sulfonylprop-2-enenitrile
SDF Download SDF
Canonical SMILES CC1=CC=C(C=C1)S(=O)(=O)C=CC#N
Shipping Condition Ship with blue ice, or upon other requests.
General tips For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

 

APExBIO台灣代理商

https://www.apexbt.com/

APExBIO提供優質的胜肽和生物分析試劑,廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。

品項 CAS# #貨號 應用
Z-VAD-FMK  CAS# 187389-52-2 貨號 A1902

Proteases

ICE-like proteases

TAPI-1  CAS#171235-71-5 貨號 B4686

Proteases

TACE/ADAM17 inhibitor Product Citation+3

Talabostat mesylate CAS# 150080-09-4 貨號B3941

Proteases

DPP4 二肽基肽酶特異性抑制劑

Z-IETD-FMK CAS# 210344-98-2 貨號B3232 inhibitor of caspase 8
Cisplatin CAS# 15663-27-1 貨號A8321 高效、廣譜的化療藥物
17-DMAG (Alvespimycin) HCl CAS#467214-21-7  貨號A2213 Hsp90 inhibitor
CA-074 Me  CAS# 147859-80-1 貨號A8239 Cathepsin B inhibitor
VX-765 CAS# 273404-37-8 貨號A8238 Caspase-1 inhibitor

 

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

ARCA Cy5 EGFP mRNA (5-moUTP) APExBIO  貨號R1009

5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization.

APEXBIO.jpg

原理與-300x92.jpg

5-moUTP 修飾的 mRNA,具有更好的性能,Cy5 標籤,直接檢測報告 mRNA,抑制 RNA 介導的先天免疫激活,用作確定 mRNA 遞送和定位的對照和工具。ARCA Cy5 EGFP mRNA (5-moUTP) 可用於分析 mRNA 傳遞(mRNA delivery)和翻譯效率(translation efficiency)。 EGFP mRNA 將表達增強版的綠色螢光蛋白(green fluorescent protein),該蛋白最初是從水母維多利亞水母(jellyfish)中分離出來的。 EGFP 是一種直接檢測報告基因(reporter gene),常用於哺乳動物細胞培養,可產生亮綠色螢光,發射峰位於 509 nm。可以直接看到用花青 5 標記的 EGFP mRNA。 ARCA Cy5 EGFP mRNA (5-moUTP) 是用於確定 mRNA 遞送和定位的理想分子,並且獨立於轉譯作用(independent of translation) 。Cyanine 5 是一種合成螢光染料,最大激發(excitation)和發射波長(emission)分別為 650 nm 和 670 nm。 APExBIO ARCA Cy5 EGFP mRNA (5-moUTP) 以 1:3 的比例與花青 5-UTP(Cyanine 5-UTP):5-甲氧基-UTP(5-methoxy-UTP) 轉錄。以這個比例替代會導致 mRNA 易於可視化,並且仍然可以在細胞培養中進行翻譯。翻譯效率與花青 5-UTP replacemen相反。ARCA Cy5 EGFP mRNA (5-moUTP) 使用 APExBIO 專有的共轉錄加帽方法(co-transcriptional capping method)進行限制,以產生具有高封端效率的天然存在的 Cap 0 結構。它是聚腺苷酸化(polyadenylation),用 5-甲氧基尿苷(5-methoxyuridine)修飾,並針對哺乳動物系統進行了優化。它模仿完全加工的成熟 mRNA(mature mRNA)。

ARCA Cy5 EGFP mRNA (5-moUTP)

 

引用文獻1-300x95.jpg

  1. Yan Cao, Zongxing He, et al. "Helper-Polymer Based Five-Element Nanoparticles (FNPs) for Lung-Specific mRNA Delivery with Long-Term Stability after Lyophilization." Nano Lett. 2022 Aug 24;22(16):6580-6589.PMID:35969167
  2. Chen Q, Gao M, et al. "Biodegradable nanoparticles decorated with different carbohydrates for efficient macrophage-targeted gene therapy." J Control Release. 2020 Apr 22;323:179-190.PMID:32334322
mRNA Length 996 nucleotides
Concentration 1.0 mg/mL
Buffer 1 mM Sodium Citrate, pH 6.4 Storage -40°C or below
General tips Dissolve it on ice and take care to prevent RNase contamination degradation. Avoid repeated freezing and thawing as much as possible. Do not vortex. For the first time, it is gently centrifuged and divided into several parts for stand-alone use. Use RNase-free reagents and consumables, using appropriate RNase-free technology. It can not be added to the serum-containing medium until it is mixed with the transfection reagent.
Shipping Condition Trial Pack: Dry Ice Transport.

操作手冊2.jpg

https://www.apexbt.com/

APExBIO台灣代理商

APExBIO提供優質的勝肽和生物分析試劑,廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。

搶購.jpg

DNA Damage/DNA Repair CAS# #貨號 應用
SCR7 CAS#1533426-72-0 貨號A8705 DNA ligase IV inhibitor
RHPS4n CAS#390362-78-4 貨號B6186 Telomerase inhibitor
AZD6738 CAS#1352226-88-0 貨號B6007 ATR inhibitor

ORY-1001

 

CAS#1431326-61-2 貨號B5882 Selective inhibitor of KDM1A.
RGFP966 CAS#1357389-11-7;1396841-57-8 貨號A8803 Specific HDAC3 inhibitor
Oxaliplatin CAS#61825-94-3 貨號A8648 Antitumor agent
(S)-Crizotinib CAS#877399-52-5;1374356-45-2

貨號A8802

 

Potent MTH1 inhibitor
Tubastatin A CAS#1252003-15-8 貨號A4101 HDAC6 inhibitor,potent and selective
APExBIO產品線 品項 CAS# #貨號
Proteases Z-VAD-FMK | CAS# 187389-52-2 APExBIO  CAS# 187389-52-2 貨號 A1902
Proteinase K 蛋白酶 K (Proteinase K) | CAS#39450-01-6 APExBIO  貨號K1037 CAS#39450-01-6 貨號K1037
品項 CAS# #貨號 應用
Romidepsin (FK228, depsipeptide)

CAS#128517-07-7

 

貨號A8173 HDAC1/HDAC2,強效和選擇性抑制劑
Pirarubicin CAS#72496-41-4 貨號B2295  anthracycline anti-neoplastic doxorubicin
Tunicamycin CAS# 15663-27-1

貨號A8321

 

高效、廣譜的化療藥物
Azithromycin CAS#83905-01-5  貨號A2213 Hsp90 inhibitor
Bleomycin Sulfate

CAS# 9041-93-4

 

貨號B1398 Antibiotic by inhibiting protein synthesis
Nystatin (Fungicidin) CAS#1400-61-9 貨號B1993 antifungal antibiotic
Rapamycin CAS#53123-88-9 貨號A8167 Original antifungal antibiotic
Daptomycin CAS#103060-53-3 貨號A1206 Calcium-dependent antibiotic
品項 CAS# #貨號 應用
Z-VAD-FMK   CAS# 187389-52-2 貨號 A1902

Proteases

ICE-like proteases

TAPI-1   CAS#171235-71-5 貨號 B4686

Proteases

TACE/ADAM17 inhibitor Product Citation+3

Talabostat mesylate CAS# 150080-09-4 貨號B3941

Proteases

DPP4 二肽基肽酶特異性抑制劑

Z-IETD-FMK CAS# 210344-98-2 貨號B3232 inhibitor of caspase 8
Cisplatin CAS# 15663-27-1 貨號A8321 高效、廣譜的化療藥物
17-DMAG (Alvespimycin) HCl CAS#467214-21-7  貨號A2213 Hsp90 inhibitor
CA-074 Me  CAS# 147859-80-1 貨號A8239 Cathepsin B inhibitor
VX-765 CAS# 273404-37-8 貨號A8238 Caspase-1 inhibitor

 

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

大鼠 D-二聚體 ELISA 試劑盒| Rat D-Dimer ELISA Kit Abbexa貨號abx256862 (7.81 ng/ml - 500 ng/ml)

原理與知識.jpg

D-二聚體(D-dimers)的獨特之處在於它們是已被因子 XIII (Factor XIII)穩定的纖維蛋白網(fibrin mesh)的分解產物。該因素將 E 元素與兩個 D 元素交聯。這是血栓(thrombosis)形成的最後一步。纖溶酶(fibrinolytic enzyme)是一種纖維蛋白溶解酶,可組織凝塊並分解纖維蛋白網(fibrin mesh)。但是,它不能破壞一個 E 單元(E units)和兩個 D(D units) 單元之間的鍵。因此留下的蛋白質片段是D-二聚體(D-dimer)。 D-二聚體(D-dimer)濃度可以通過驗血來確定,以幫助診斷血栓(thrombosis)形成。自 1990 年代推出以來,它已成為對疑似血栓性疾病患者進行的一項重要測試。雖然陰性結果實際上排除了血栓(thrombosis)形成,但陽性結果可能表明血栓(thrombosis)形成,但不排除其他潛在原因。因此,它的主要用途是排除可能性低的血栓栓塞性疾病(thromboembolic disease)。

D-二聚體(D-dimer)是由纖維蛋白(fibrin protein)的兩個 D 片段(D fragments)通過交聯(cross-link)連接而成的蛋白質。 D-二聚體(D-dimer)是幾種纖維蛋白降解產物 (FDP) 中的一種,由纖維蛋白溶解(fibrinolysis)降解血凝塊(blood clot)形成。它用於診斷血液疾病彌散性血管內凝血(blood disorder disseminated intravascular coagulation)和血栓(thrombosis)形成的診斷。該試劑盒設計用於定量測量小鼠血漿中的纖維蛋白原 D-二聚體蛋白(Fibrinogen D-Dimer protein)。

LINE群發(RT-9600).jpg

操作手冊2.jpg

Introduction D-dimer is a protein formed by two D fragments of the fibrin protein joined by a cross-link. D-dimer is one of several fibrin degradation product (FDP) formed by the degradation of a blood clot by fibrinolysis. It is used in the diagnosis of the blood disorder disseminated intravascular coagulation and in the diagnosis of thrombosis. This kit is designed for the quantitative measurement of Fibrinogen D-Dimer protein in Rat Serum, plasma and other biological fluids.
Target D-Dimer
Reactivity Rat
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is 6 months.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range 7.81 ng/ml - 500 ng/ml
Sensitivity 4.69 ng/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Competitive
Assay Data Quantitative
Sample Type Plasma.
Kit Components

The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Sample Collection/Preparation
  • Serum: Samples should be collected into a serum separator tube. Coagulate the serum by leaving the tube undisturbed in a vertical position overnight at 4°C or at room temperature for up to 60 minutes. Centrifuge at approximately 1000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20°C or -80°C.
  • Plasma: Collect plasma using heparin or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 × g within 30 minutes of collection. Assay immediately or aliquot and store at -20°C or -80°C. Avoid hemolysis and high cholesterol samples.
  • Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type – this is just an example. Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh before homogenization. Finely mince tissues and homogenize with a tissue homogenizer on ice in PBS and sonicate the cell suspension. Centrifuge the homogenates at 5000 × g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20°C.
Reagent Preparation

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
  • 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
  • 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
Assay Procedure

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 50 µl of diluted standard into the standard wells.
  • 3) Aliquot 50 µl of Standard Diluent buffer into the control (zero) well.
  • 4) Aliquot 50 µl of diluted samples into the sample wells.
  • 5) Immediately aliquot 50 µl of Detection Reagent A to each well. Incubate for 45 mins at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of D-Dimer were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of D-Dimer were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Availability Shipped within 5-7 working days.
Note This product is for research use only.The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with Antigen

相關產品-300x96.jpg

血尿素氮(Blood Urea Nitrogen , BUN)檢測 | 肌酐檢測試劑盒(Creatinine Assay Kit)  | UA尿酸檢測試劑盒 | Calcium鈣檢測試劑盒 | 鎂檢測試劑盒

貨號 (分析套組) 分析品項 產品資料下載
abx090684 血尿素氮(Blood Urea Nitrogen , BUN)檢測 操作手冊
abx298944 肌酐檢測試劑盒(Creatinine Assay Kit)  操作手冊
abx298824 UA尿酸檢測試劑盒 操作手冊
abx298871 Calcium鈣檢測試劑盒 操作手冊
abx298873 鎂檢測試劑盒 操作手冊
abx298918 亞硝酸鹽檢測試劑盒| Nitrite Assay Kit貨號abx298918 操作手冊
abx298986 總碳水化合物檢測試劑盒 操作手冊
abx096001 ALT丙氨酸轉氨酶檢測試劑盒| Alanine Transaminase assay kit 操作手冊
abx294012 髓過氧化物酶 (MPO) 檢測試劑盒| Myeloperoxidase (MPO) Assay Kit 操作手冊
 abx298894 硫醇檢測試劑盒 操作手冊
 abx298878 孔雀石綠磷酸鹽檢測試劑盒 操作手冊
 abx298825 銅藍蛋白檢測試劑盒| Ceruloplasmin Assay Kit 操作手冊
 abx097195 即用型IHC 檢測試劑盒| IHC Detection Kit for Mouse and Rabbit Primary Antibody 操作手冊
abx298849 脂肪酶檢測試劑盒| Lipase Assay Kit貨號abx298849 操作手冊
abx097981 硫代巴比妥酸反應物質 (TBARS) 檢測試劑盒| Thiobarbituric Acid Reactive Substances (TBARS) Assay Kit 操作手冊
abx298964 甲醛檢測試劑盒| Formaldehyde Assay Kit 操作手冊
abx298847 胃蛋白酶檢測試劑盒| Pepsin Assay Kit 操作手冊
abx298934  總酚測定試劑盒| Total Phenols Assay Kit 操作手冊
abx298947 糖化血清蛋白檢測試劑盒 | Glycated Serum Protein Assay Kit 操作手冊
abx298913 植酸酶檢測試劑盒 | Phytase Assay Kit 操作手冊
abx298889 糖原/肝糖檢測試劑盒 | Glycogen Assay Kit貨號abx298889 操作手冊
abx298916 果糖胺檢測試劑盒 | fructosamine Kit 操作手冊
abx298937 色氨酸 (Trp) 檢測試劑盒 | The Tryptophan (Trp) Assay Kit 操作手冊
abx298911 膽紅素檢測試劑盒 | Bilirubin Assay Kit 操作手冊
abx298941  類黃酮檢測試劑盒 | Flavonoid Assay Kit 操作手冊
abx298951 牛磺酸檢測試劑盒 | Taurine Assay Kit 操作手冊
abx298950 脂氧合酶檢測試劑盒 | The Lipoxygenase Assay Kit 操作手冊
abx298806 醛脫氫酶 (ALDH) 檢測試劑盒 | Aldehyde Dehydrogenase (ALDH) Assay Kit 操作手冊
abx298912 乙醇檢測試劑盒 | Ethanol Assay Kit 操作手冊
abx298966 唾液酸檢測試劑盒 | Sialic Acid Assay Kit 操作手冊
abx298811 肌酸激酶 (CK) 檢測試劑盒 | Creatine Kinase (CK) Assay Kit 操作手冊
abx299040 人末端脫氧核苷酸轉移酶 (TDT) 免疫螢光試劑盒 操作手冊
abx291003 乳膠珠 | Latex Beads 操作手冊
貨號 (合成胜肽) 胜肽品項 產品資料下載
貨號abx069990 專利hTERT 胜肽(研究用) | hTERT Peptide 操作手冊
貨號abx266593 Substance P (5-11) / Hepta-Substance P Peptide 操作手冊
貨號 abx266594 Tax8, HTLV 1 (12 19) Peptide胜肽 操作手冊
貨號 abx266634 纖維蛋白原相關胜肽Fibrinogen Related Peptide 操作手冊
貨號abx265240 Ac-Angiotensinogen (1-14) Peptide胜肽 操作手冊
貨號abx265234 c-Calpastatin (184-210)Peptide胜肽 操作手冊
貨號abx266749 Ac-Choline Receptor α1(129-145) peptide胜肽  操作手冊
貨號 abx265411 Ac-d-Endorphin peptide胜肽 操作手冊
貨號 abx266742 Ac-g-Endorphin胜肽  操作手冊
貨號  abx265898 Ac-Glutamine胜肽 操作手冊
貨號  abx266677 Ac-LEHD-pNA胜肽  操作手冊
貨號abx266337 Ac-MBP (4-14) Peptide胜肽 操作手冊
貨號 abx265332 Ac-Neurotrophin Receptor (368-381) amide Peptide胜肽 操作手冊
貨號abx266543 Ac-Pepstatin Peptide胜肽 操作手冊
貨號abx266157 Ac-Phe-Gly-pNA Peptide胜肽  操作手冊
貨號abx066547 Ferritin, Heavy Polypeptide (FTH)胜肽 操作手冊
貨號 abx065888 重組絨毛膜促性腺激素α多肽 操作手冊
貨號 abx165811 鬆弛素 2 蛋白 操作手冊
貨號abx165596 三文魚降鈣素 (CALCA) 胜肽 (KLH)  操作手冊
貨號abx653518 小鼠胃抑制多肽 (GIP)胜肽 操作手冊
貨號 abx165831 人胸腺素 Beta 4 (TMSB4)胜肽OVA  操作手冊
貨號abx165502 人腎上腺髓質素 (ADM) 胜肽 (KLH) 操作手冊
貨號abx652286 小鼠前膠原 I N-末端前肽 (PINP)胜肽 操作手冊
貨號abx652288 胰多肽Pancreatic Polypeptide胜肽  操作手冊
貨號 abx267063 β 澱粉樣蛋白 (1-49)胜肽  操作手冊
貨號abx265135 β-Amyloid (1-42)胜肽 操作手冊
貨號abx265118 Ac beta 內啡肽 操作手冊
貨號abx265595 Ac-DEVD-AMC  | Caspase 3 Substrate  操作手冊
貨號abx265662 AKT/PKB/Rac  | Protein Kinase Substrate 操作手冊
貨號abx265648 2B / 3,登革熱蛋白酶底物肽  | 2B/3, Dengue Protease Substrate  操作手冊
文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

NEW 細胞內脂質染色套組 (套組包括Hematoxylin染核Lipid (Oil Red O) staining Kit Abcam ab287838

脂質染色套組 貨號K580-24  | 24ML  Lipid (Oil Red O) staining Kit Abcam ab287838 (包括Hematoxylin供細胞染核)

Biovision提供一系列脂質分析套組;細胞內脂質染色套組(Lipid (Oil Red O) staining Kit, K580),可用於細胞內中性脂質之染色;培養型態之細胞: 如脂肪細胞(adipocytes),肝細胞(hepatocytes)及巨噬細胞(macrophages)。提供細胞內直接染色或半定量分析。脂滴(Lipid droplets )發現在所有的真核生物(eukaryotic organisms)中,它們作為中性脂質(neutral lipids)的儲庫,主要是甘油三酯 ( triacylglycerol) 和膽固醇酯 (cholesterol esters)。 儲存的脂質可用於能量(energy),類固醇合成(steroid synthesis)或膜形成(membrane formation)。 脂質液滴積聚(Lipid droplet accumulation)是細胞中的正常功能。 脂肪細胞(Adipocytes)主要由脂滴(ipid droplets)組成; 然而,細胞內過多的脂滴積聚可能是代謝缺陷( metabolic deficiency )或發病機制 (pathogenesis)的指標。 例如,肝細胞中脂質的過量積累脂肪變性(steatosis)可導致細胞功能障礙(cellular dysfunction)。 在動脈粥樣硬化(atherosclerosis)開始時,巨噬細胞(macrophages )吞噬氧化型低密度脂蛋白(oxidized-LDL,),發展成為泡沫細胞,並有助於動脈狹窄(artery narrowing)。 BioVision的Lipid(油紅O)染色試劑盒可以選擇性檢測培養細胞中的中性脂質。 該試劑盒還包括蘇木精(Hematoxylin) 染色核。 提供足夠的試劑來染色兩(2)96-well plates,兩(2) 6-well plates或四(4) 100 mm culture dishes。

同質商品7.jpg

Lipid (Oil Red O) staining Kit | BioVision Inc.

 

操作手冊2.jpg

BioVision’s Lipid (Oil Red O) Staining Kit allows selective detection of neutral lipids within cultured cells. The kit also includes Hematoxylin to stain nuclei. This kit provides enough reagents to stain two (2) 96-well plates, two (2) 6-well plates, or four (4) 100 mm culture dishes.
k580-a.png

細胞內脂質染色套組 | K580-24內容物

  • PBS  48ml
    • Formalin (10%) 24ml
    • Oil Red O  60mg
    • Hematoxylin 24ml

更多脂質相關分析產品

 Catalog #  Product Name+  Size
 K579-100  3T3-L1 Differentiation Kit  For 100 ml differentiation medium
 K658-100  Acetate Colorimetric Assay Kit  100 assays
 K650-100  Acetoacetate Colorimetric Assay Kit  100 assays
 K598-100  Acid Sphingomyelinase Activity Colorimetric Assay Kit  100 assays
 K610-100  Adipogenesis Colorimetric/Fluorometric Assay Kit  100 assays
 K4901-100  Adiponectin (human) Elisa Assay Kit  96 assays
 K4902-100  Adiponectin (mouse) Elisa Assay Kit  96 assays
 K4903-100  Adiponectin/gACRP30 (rat) Elisa Assay Kit  96 assays
 K731-100  Aldehyde Dehydrogenase Activity Colorimetric Assay Kit  100 assays
 K4696-100  Apolipoprotein E (human) ELISA Kit  100 assays
 K601-100  CETP Activity Fluorometric Assay Kit  100 assays
 K595-100  CETP Activity Fluorometric Assay Kit II  100 assays
 K602-100  CETP Inhibitor Drug Screening Kit (Fluorometric)  100 assays
 K594-100  CETP Inhibitor Screening Kit (Fluorometric) II  100 assays
 K582-100  Cholesterol Efflux Fluorometric Assay Kit (cell-based)  100 assays
 K7430-100  Cortisol (human/mouse/rat) ELISA Kit  100 assays
 K7434-100  Cortisol (Saliva) Human ELISA Kit  100 assays
 K612-100  Free Fatty Acid Quantification Colorimetric/Fluorometric Kit  100 assays
 K634-100  Free Glycerol Colorimetric Assay Kit II  100 assays
 K630-100  Free Glycerol Colorimetric/Fluorometric Assay Kit  100 assays
 K4921-100  FTO (human intracellular) ELISA Kit  100 assays
 K4922-100  FTO (mouse intracellular) ELISA Kit  100 assays
 K641-100  Glycerol-3-Phosphate (G3P) Colorimetric Assay Kit  100 assays
 K640-100  Glycerol-3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit  100 assays
 K613-100  HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit  100 assays
 K586-100  HDL Uptake Assay Kit (Fluorometric)  100 assays
 K584-100  Hepatic Steatosis Assay Kit  100 assays
 K588-100  HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric)  100 assays
 K632-100  β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit  100 assays
 K4761-100  Irisin Competitive ELISA Kit  100 assays
 K585-100  LDL Uptake Assay Kit (Fluorometric)  100 assays
 K4777-100  Leptin (human) ELISA Kit  100 assays
 K722-100  Lipase Activity Colorimetric Assay Kit  100 assays
 K723-100  Lipase Activity Colorimetric Assay Kit II  100 assays
 K724-100  Lipase Activity Fluorometric Assay Kit III  100 assays
 K580-24  Lipid (Oil Red O) staining Kit  24 ml
 K577-100  Lipolysis (3T3-L1) Colorimetric Assay Kit  100 assays
 K581-5  Lipolysis (Adipocyte) Colorimetric/Fluorometric Assay Kit  For 5 g Tissue
 K721-100  Lipoprotein Lipase Activity Fluorometric Assay Kit  100 assays
 K4909-100  Nampt (Visfatin/PBEF) (human) Intracellular ELISA Kit  100 assays
 K4908-100  Nampt (Visfatin/PBEF) (mouse/rat) Serum ELISA Kit  100 assays
 K766-100  PAF Acetylhydrolase (PAF-AH) Inhibitor Screening Kit (Colorimetric)  100 assays
 K765-100  PAF Acetylhydrolase Activity Assay Kit (Colorimetric)  100 assays
 K7265-100  PCSK9 (human) ELISA Kit  100 assays
 K7266-100  PCSK9 (mouse) ELISA Kit  100 assays
 K576-100  Phosphatidylcholine Colorimetric/Fluorometric Assay Kit  100 assays
 K725-100  Phospholipase D (PLD) Activity Colorimetric Assay Kit  100 assays
 K741-100  PicoProbe™ Aldehyde Dehydrogenase Activity Fluorometric Assay Kit  100 assays
 K673-100  PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit  100 assays
 K643-100  PicoProbe™ Free Glycerol Fluorometric Assay Kit  100 assays
 K651-100  PicoProbe™ β-Hydroxybutyrate Fluorometric Assay Kit  100 assays
 K578-100  PicoProbe™ Lipolysis (3T3-L1) Fluorometric Assay Kit  100 assays
 K614-100  PicoProbe™ Triglyceride (TG) Fluorometric Assay Kit  100 assays
 K593-100  PLTP Activity Fluorometric Assay Kit II  100 assays
 K605-100  PLTP Inhibitor Drug Screening Kit (Fluorometric)  100 assays
 K583-5  Preadipocyte Isolation Kit  For 5 g Tissue
 K4911-100  RBP4 (human) Competitive ELISA Kit  100 assays
 K4912-100  RBP4 (human) ELISA Kit  100 assays
 K4913-100  RBP4 (mouse/rat) ELISA Kit  100 assays
 K4767-100  Resistin (human/mouse/rat) EIA Kit  100 assays
 K600-100  Sphingomyelin Quantification Colorimetric Assay Kit  100 assays
 K599-100  Sphingomyelinase Activity Colorimetric Assay Kit  100 assays
 K574-100  Sphingomyelinase Activity Fluorometric Assay Kit  100 assays
 K623-100  Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II  100 assays
 K603-100  Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit  100 assays
 K622-100  Triglyceride Quantification Colorimetric/Fluorometric Kit  100 assays
 K4907-100  Visfatin (human) Elisa Assay Kit  100 assays

代謝相關商品

碳水化合物和脂質代謝 | 胺基酸與無機離子代謝 | 糖酵解途徑和中介代謝|氧化傷害與血清成分分析

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

Amylase活性呈色分析套組 貨號K711-100 (alpha-amylase activity)  (abcam ab102523)

Amylase Activity Colorimetric Assay Kit

澱粉酶 (Amylases )是將澱粉分解成糖分子 ( sugar molecules) 的酶 (enzymes )。 α-澱粉酶 ( α-amylase) 是人和其他哺乳動物中發現的澱粉酶的主要形式,以及存在於種子 (seeds) 或真菌 ( fungi ) (例如麵包酵母)中的酶。 α-澱粉酶是鈣金屬酶 (calcium metalloenzyme) ,完全不能在沒有鈣 ( calcium) 的情況下起作用。在人體生理學中,唾液 ( salivary) 和胰澱粉酶 ( pancreatic amylases) 都是主要的消化酶。人類增加的酶水平與唾液創傷有關;腮腺炎由於唾液腺的炎症,胰腺炎 ( pancreatitis) 和腎衰竭 (renal failure) 。 BioVision的α-澱粉酶測定使用亞乙基-PNP-G7 (ethylidene-pNP-G7) 作為底物。一旦底物被α-澱粉酶特異性切割,產生的較小的片段可以被α-葡糖苷酶 (α-glucosidase) 作用,導致髮色團 ( chromophore ) 的最終釋放,可於405nm測量。該檢測法可檢測低至0.2 mU的α-澱粉酶 (α-amylase) 含量。

操作手冊2.jpg

 

同質商品7.jpg

Size 100 assays
SKU+Size K711-100
Detection Method Absorbance (405 nm)
Species Reactivity Mammalian
Applications The assay can detect α-amylase content as low as 0.2 mU.
Features & Benefits • Simple procedure; takes ~ less than 40 minutes
• Fast and convenient

為了能夠計算樣品中的澱粉酶活性,必須獲得std曲線的線性範圍內的值。 std的最高數據點是每孔20 nmol硝基酚含量。 單位定義:一個單位的澱粉酶是切割亞乙基-pNP-G7 (ethylidene-pNP-G7) 的澱粉酶的量在pH 7.20,25℃下每分鐘產生1.0μmol硝基苯酚。std曲線的線性範圍內的酶活性的最大量為每孔20mU。Calculation: Plot the Nitrophenol standard curve. Apply the ∆OD (∆OD = ODT1 -ODT0) to the Nitrophenol standard curve to get B nmol of Nitrophenol generated by amylase between T0 and T1.

LINE群發(RT-9600).jpg

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

活性氧(ROS)檢測試劑盒 貨號K936 (Abcam ab287839)

k936-3.jpg

Reactive Oxygen Species (ROS) Detection Assay Kit

 

原理與知識.jpg

 

 

不斷產生低水平的活性氧(ROS)和自由基是所有活細胞的基本特徵。低水平的ROS在信號傳導途徑中起重要作用,而在氧化應激下ROS活性增加,導致對核酸,蛋白質和膜脂的破壞。 ROS在氧化應激過程中的積累也與衰老,細胞凋亡或壞死有關,並與病理狀態有關,如:血管疾病,糖尿病,腎缺血,動脈硬化,肺病,炎性疾病和癌症。 ROS的細胞活性被抗氧化劑,多種修復系統和受損DNA的替換所抵消。細胞內ROS水平的探針測量,提供了研究氧化應激誘導劑和抗氧化劑治療效果的重要工具。 BioVision的ROS檢測試劑盒設計用於檢測活細胞中的羥基,過氧化氫或其他活性氧。我們利用H2DCFDA,一種獨特的細胞滲透螢光探針,與酚紅,FBS和BSA兼容,以檢測活細胞中的活性氧。細胞進入後,H2DCFDA被細胞酯酶修飾以形成非螢光的H2DCF。 H2DCF被細胞內ROS氧化產生高度螢光的產物,可通過FACS,酶標儀或螢光顯微鏡(Ex / Em 495 / 529nm)檢測。螢光強度與ROS水平成正比。我們的試劑盒為活細胞中ROS測量提供了一種簡單而具體的測定方法。產品具高靈敏度,特異性和準確度,可作為測量ROS水平或抗氧化劑活性之常見ROS誘導劑。

同質商品7.jpg

k936-1.jpg

操作手冊2.jpg

Kit Summary • Detection method- Fluorescence (Ex/Em = 495/529 nm).
• Applications- Measurement of intracellular levels of ROS
• Screening compounds with a stress inducing and/or antioxidant function.
Detection Method Fluorescence (Ex/Em = 495/529 nm).
Species Reactivity Eukaryotes
Applications • Measurement of intracellular levels of ROS.
• Screening compounds with a stress inducing and/or antioxidant function
Features & Benefits • Simple & Rapid Protocol
• Convenient: Non-Radioactive, no special handling or disposal required
• High-Throughput
• Accurate: reproducible results with low intra & inter assay variability
Kit Components • ROS Assay Buffer
• ROS Label (1000X)
• ROS Inducer (250X)
文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

D-葡糖酸呈色分析套組  D-Gluconate (D-Gluconic Acid) Assay Kit (Colorimetric)   | 貨號K683-100 (abcam ab204703)

 

biovision商品插圖-300x207.jpg

D-葡糖酸呈色分析套組 貨號K683-100 | 100個分析 產品描述: Biovision提供一系列碳水化合物分析套組;其中D-葡糖酸呈色分析套組,吸收光Absorbance (450 nm)偵測;可用於Animal tissues動物組織培養液(e.g. muscle etc.)、Wine (e.g. red wine and white wine)、Fruits (e.g. orange, apple etc.)等來源。測量最低靈敏度2 µM of D-Gluconic acid。

LINE群發(RT-9600).jpg

產品描述:D-葡萄糖酸(C 6 H 12 O 7)是由葡萄糖氧化酶從葡萄糖產生的溫和有機酸。 它大量存在於植物,果實和動物組織中。 D-葡萄糖酸鹽(C6H11O7)是葡糖酸的鹽或酯。 由於其低毒性,它廣泛應用於製藥,食品等行業。 BioVision的D-葡萄糖酸(葡萄糖酸)呈色分析試劑盒是一種靈敏、快速和易於使用的試劑盒。 在該測定中,葡萄糖酸被葡萄糖激酶用於形成D-葡萄糖酸-6-P和ADP,其隨後經歷一系列反應以形成還原葡萄糖酸鹽探針的中間體,得到在450nm具有強吸光度的產物。 該測定試劑盒可檢測各種樣品中的D-葡萄糖酸(D-葡萄糖酸)水平低於2μM。

操作手冊2.jpg

 Biovison品牌 貨號 試劑品項 包裝 操作手冊下載
Biovision 1041 Cycloheximide蛋白質合成抑制劑 | 貨號1041-1G   1ML/1G/5G 1041
Biovision 1786 EZSolution™ SB 203580  貨號1786-1 1mg 1862
Biovision 1748 EZSolution™ Y-27632  貨號1784-5 5mg 1784
Biovision 1755 Calcein AM 活細胞螢光染劑 | 貨號1755-250 | 規格50ugX5 50ug/5x50ug/1mg 1755
 Biovision 1738 ASB-14 A zwitterionic detergent兩性離子洗滌劑 | 貨號1738 5g/25g 1738
Biovision 1714 IBMX |  CAS28822-58-4貨號1714 100mg 1714
Biovision 1708 Akt抑制劑Isozyme-Selective | 貨號1708-1 1mg 1708
Biovision 1692 Lovastatin| 降膽固醇藥物貨號1692-250  A HMG-CoA inhibitor 50mg/250mg 1692
Biovision K280 線粒體DNA分離套組 | 貨號K280-50 50 assays K280
Biovision 1691 Metformin, Hydrochloride | 貨號1691-5G   An AMPK activator 1g/5g/50g 1691
Biovision 1649 IPTG, Animal-Free (Dioxane-Free) | 貨號1649-5G  1g/5g/50g 1649
 Biovision  1643  PD-0325901 | MEK選擇性非競爭型抑制劑| 貨號1643-25   A MEK inhibitor    2mg/5mg/25mg  1643
Biovision 1568 Rapamycin免疫抑制劑 | 貨號1568-50 50mg   5mg/50mg 1568
Biovision 1557 抗生素系列產品  | G418 sulfate | G418抗生素 | Antibiotic G418  1g/5g/25g 1557
Biovision 1550 Cisplatin | 貨號1550-1000   An anticancer agent 100mg/1g 1550
Biovision 1548 PMSF | 貨號1548 CAS# 329-98-6 5g/25g 1548
Biovision 1545 高純度CHAPS 兩性離子非變性洗滌劑 | CAS#75621-03-3貨號1545 10g/50g/100g/500g/1kg 1545
Biovision 1522 Cyclosporine A | 貨號1522-1G   An immunosuppressant 1g 1522
Biovision 1218 anti-fading封片劑 FluorLast™ with DAPI and DABCO (FRASC) | 貨號1218-20 20ml 1218
Biovision 1217 anti-fading封片劑 FluorLast™ with DABCO (FRASC) | 貨號1217-20 20ml 1211
Biovision 1211 "強效型" 螢光封片劑 SuperMOUNT™ Mounting Medium| 貨號1211-20 20ml 1211
Biovision B1990 Robenacoxib | 化合物CAS# 220991-32-2貨號B1990-25 25ml B1990
Biovision 1209 Trypan Blue (0.4%) 台盼藍死細胞染色劑 貨號1209-10 | 規格10ml (0.4%) 10ML 1209
Biovision 1140 Z-VAD-FMK | 貨號1140-1MG 1mg/5mg 1140
Biovision 1135 Cathepsin L, human recombinant組織蛋白酶L貨號1135-100 5 µg /100 µg/ 1 mg 1135
Biovision 1130 JC-1 粒腺體染劑 | 貨號1130-5 5mg 1130
Biovision K816 螢光蛋白(fluorescent proteins)套組 貨號K816-6-100 100ug x6  k816
Biovision  K658  醋酸比色分析試劑盒 | 貨號K658-100 100 assays  K658
Biovision  1056  Propidium Iodide 死細胞核酸染劑 | 貨號1056 1ml (1mg/ml)  1056 PI染劑
Biovision  K742  Tyrosinase酪氨酸酶活性測定試劑盒(呈色法) | 貨號K742-100 100 assays  6511 protein G Sepharose
Biovision  K1463  Coronavirus SARS-CoV-2 IgM / IgG抗體檢測卡/快篩試劑 貨號K1463 25 assays  K1463
Biovision  K787  乙醇脫氫酶測定試劑盒  | 貨號K787-100 100 assays  K787
Biovision  K731  醛脫氫酶活性比色測定試劑盒 | 貨號K731-100 100 assays  K731
Biovision  2589  Hygromycin B  | 貨號2589 500mg, 1g  2589
Biovision  K761  Glutathione Reductase Activity Colorimetric Assay Kit呈色分析套組 |貨號K761-200 200 assays  K761
Biovision  K762  GPx呈色分析套組Glutathione Peroxidase Activity Colorimetric Assay Kit 100 assays  K762
Biovision  K952  TG甘油三酯定量試劑盒 | Triglyceride Quantification Colorimetric Assay Kit (384-well) |  貨號K952 100 assays  K952
Biovision  K629  Glutamate麩胺酸檢測 | 貨號K629-100 100 assays  K629
Biovision  7903  D-Luciferin Potassium Salt  D-螢光素鉀鹽 | 貨號7903-1G 1g, 5x1g  7903
Biovision  1557  抗生素系列產品  | G418 sulfate | G418抗生素 | Antibiotic G418 1557 1 g, 5 g, 25 g, 100 g  1557
Biovision  2829  Taurocholic acid sodium salt牛磺酸鈉 | 貨號2829 5 g, 25 g, 100 g  2829
Biovision  K568  焦磷酸鹽(PPi)分析試劑盒 | 貨號K568-100 100 assays  K568
Biovision  1215  (TMB) 3,3'5,5'-四甲基聯苯胺| 貨號1215-100 100ml  1215
Biovision  K4219  Dopamine多巴胺檢測 (酵素免疫分析) | 貨號K4219   96 assays  K4219
Biovision  K544  亞硝酸鹽檢測試劑盒 (Griess Reagent) | 貨號k544-200  200 assays  K544
Biovision  1010-100  Caspase-Family Inhibitor Z-VAD-FMK/ pan-caspase inhibitor | 貨號1010-100 100 µl (2 mM) Z-DEVD-FMK in DMSO  1010-100
Biovision  K295  Cell Surface Protein Isolation Kit 細胞表面蛋白分離試劑盒 | 貨號K295 10 assays  åŽŸå» æ“ä½œä¸‹è¼‰
Biovision  K475  Polyamines多胺螢光分析套組  | 貨號K475 100 assays  K475
Biovision  K723  脂肪酶活性比色分析試劑盒 | 貨號K723-100 100 assays  K723
Biovision  7847  TEV蛋白酶 |  貨號7847-1000 100ug, 1000ug  7847
Biovision  K410  磷酸鹽比色測定試劑盒| 貨號K410-500 500 assays  K410
Biovision  K630  (不同樣品來源) 游離甘油比色/螢光檢測試劑盒 | 貨號K630-100 100 assays  K630
Biovision  K648  Glycogen Colorimetric Assay Kit II肝醣呈色分析套組 | 貨號K648-100  (適用於還原物質的樣品) 100 assays  K648
Biovision  k250  MitoCapture線粒體細胞凋亡檢測螢光試劑盒  | 貨號K250 -100 100 assays  k250
Biovision  k983  Aromatase (CYP19A) Activity Assay Kit (Fluorometric) 芳香酶螢光分析套組  | 貨號K983-100 100 assays  k983
Biovision  k938  α-葡萄糖苷酶抑制劑篩選試劑盒 |α-Glucosidase Inhibitor Screening Kit (Colorimetric)貨號K938-100 100 assays  k938
Biovision  k482  α-澱粉酶抑制劑篩選試劑盒|α-Amylase Inhibitor Screening Kit貨號 K482 100 assays  k482
Biovision  6500B  Protein A (Lyophilized) 貨號6500B-10 10mg, 25mg, 100mg, 1g  6500b
Biovision  6510  重組蛋白G | Protein G 貨號6510 1mg, 5mg, 10mg,  100mg  6510
Biovision  k936  活性氧(ROS)檢測試劑盒 | 貨號K936 100 / 250 assays  k936
Biovision  k752  丙氨酸氨基轉移酶(ALT或SGPT)活性比色/螢光測定試劑盒 | 貨號K752-100 100 assays  k752
Biovision  k627  乳酸比色測定試劑盒II Lactate Colorimetric Assay Kit II | 貨號K627-100  0.02 mM - 10 mM乳酸 100 assays  k627
Biovision  k686  葡萄糖比色測定試劑盒II | 貨號K686-100  20μM - 10mM 100 assays  k686
Biovision  2129  PBS Tablets | 貨號2129-10 10 Tablets, 100 Tablets  2129
Biovision  1755  Calcein AM 活細胞螢光染劑 | 貨號1755-250 | 規格50ugX5 50ug, 250ug, 1000mg  1755
Biovision  k654  Malate Dehydrogenase蘋果酸脫氫酶活性比色測定試劑盒 | 貨號K654-100 10 Tablets, 100 Tablets  k654
Biovision  9210  分子等級重組蛋白酶K 100 mg | 貨號9210 100mg  9210
Biovision  k2049  碳水化合物(Carbohydrates)檢測 |  貨號K2049-100 100 mg, 500 mg, 1 g, 10 g, 50 g  k2049
Biovision  k264  GSH/GSSG螢光分析套組Glutathione (GSH/GSSG/Total) Fluorometric Assay Kit    | 貨號K264-100 100 assays  k264
Biovision  k306  BrdU細胞增殖測定試劑盒 | 貨號K306-1000 100 assays  k306
Biovision  k795  單胺氧化酶活性(總MAO / MAO-A / MAO-B)螢光測定試劑盒| 貨號K795  100 assays  k795
Biovision  1218  anti-fading封片劑 FluorLast™ with DAPI and DABCO (FRASC) | 貨號1218-20 20ml  1218
Biovision  6288  Hemocyanin-Keyhole Limpet (KLH), Native | 貨號6288-25 25mg  6288
Biovision  k818  BCA蛋白測定試劑盒 還原劑兼容 | 貨號K818-1000 1000 assays  k818
Biovision  k4210  Kanamycin ELISA分析套組  | 貨號K4210-100   100 assays  k4210
Biovision  k1012  3.5kD DiaEasy™即用型透析管 | Dialyzer (3 ml) MWCO 3.5 kDa貨號K1012  10管, 25管, 100管  k1012
Biovision  k711  Amylase活性呈色分析套組 | 貨號K711-100 (alpha-amylase activity)  100 assays k711
Biovision  k771  胰蛋白酶活性測定試劑盒 | 貨號K771  100 assays  k711
Biovision  k266  細胞核/細胞質分離試劑盒 Nuclear/Cytosol Fractionation Kit | 貨號K266-100  100 assays  k266
 Biovision  k748  Phosphatidic acid磷脂酸檢測試劑盒 | 貨號K748-100  100 assays  k748
 Biovision  k417  ATP酶活性測定試劑盒(呈色法)ATPase Activity Assay Kit (Colorimetric)  | 貨號K417-100  100 assays  k417
 Biovision  E4626  透明質酸ELISA檢測 | 貨號E4626-100  96 assays  E4626
 Biovision  1073  Caspase-2 Inhibitor Z-VDVAD-FMK | 貨號1073-20C  20 µl (10 mM), 100 µl (2 mM)  1073
 Biovision  1001  Annexin V-FITC Reagent  | 貨號1001-200  200 assays / 1000 assays  1001-1000
 Biovision  1042  Dexamethasone | CAS #50-02-2貨號1042  1g/10g/1ml  1042
 Biovision  1056  Propidium Iodide 死細胞核酸染劑 | 貨號1056  1ml  1056 PI染劑
 Biovision  1075 Caspase-9 Substrate Ac-LEHD-AFC | 貨號1075-200  200 assays / 1000 assays  1075
 Biovision  1083  Caspase-3 重組蛋白 | 貨號1083 25 units /100 units / 5 µg /10 µg  1083
 Biovision  1149  Z-LEHD-FMK | 貨號1149  1mg/5mg  1149
         
 Biovision  1035  1 X Annexin V Binding Buffer  | 貨號1035-100  100 ml  1035
 Biovision  1007  Caspase-3 Substrate DEVD-AFC | 貨號1007-200  200 assays / 1000 assays  1007-200
 Catalog #  Product Name+  Size
 K778-100  2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit  100 assays
 K658-100  Acetate Colorimetric Assay Kit  100 assays
 K665-100  Aldolase Activity Colorimetric Assay Kit  100 assays
 K711-100  Amylase Activity Colorimetric Assay Kit  100 assays
 K7430-100  Cortisol (human/mouse/rat) ELISA Kit  100 assays
 K7434-100  Cortisol (Saliva) Human ELISA Kit  100 assays
 K683-100  D-Gluconate (D-Gluconic Acid) Assay Kit (Colorimetric)  100 assays
 K644-100  D-Mannitol Colorimetric Assay Kit  100 assays
 K631-100  D-Sorbitol Colorimetric Assay Kit  100 assays
 K691-100  Enolase Activity Colorimetric/Fluorometric Assay Kit  100 assays
 K634-100  Free Glycerol Colorimetric Assay Kit II  100 assays
 K630-100  Free Glycerol Colorimetric/Fluorometric Assay Kit  100 assays
 K619-100  Fructose Colorimetric/Fluorometric Assay Kit  100 assays
 K617-100  Galactose and Lactose Colorimetric/Fluorometric Assay Kit  100 assays
 K621-100  Galactose Colorimetric/Fluorometric Assay Kit  100 assays
 K680-100  GAPDH Activity Assay Kit   100 assays
 K616-100  Glucose and Sucrose Colorimetric/Fluorometric Assay Kit  100 assays
 K686-100  Glucose Colorimetric Assay Kit II  100 assays
 K606-100  Glucose Colorimetric/Fluorometric Assay Kit  100 assays
 K676-100  Glucose Uptake Colorimetric Assay Kit  100 assays
 K666-100  Glucose Uptake Fluorometric Assay Kit  100 assays
 K697-100  Glucose-1-Phosphate (G1P) Colorimetric Assay Kit  100 assays
 K657-100  Glucose-6-Phosphate Colorimetric Assay Kit  100 assays
 K757-100  Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit  100 assays
 K681-50  GluTracker™ Glucose Uptake Assay Kit (Cell-Based)  50 assays
 K648-100  Glycogen Colorimetric Assay Kit II  100 assays
 K646-100  Glycogen Colorimetric/Fluorometric Assay Kit   100 assays
 K789-100  Hexokinase Colorimetric Assay Kit  100 assays
 K690-100  α-Glucosidase Activity Colorimetric Assay Kit   100 assays
 K733-100  β-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric)  100 assays
 K4742-100  Insulin (human) ELISA Kit  100 assays
 K674-100  Invertase Activity Colorimetric Assay Kit  100 assays
 K624-100  Lactose Colorimetric/Fluorometric Assay Kit  100 assays
 K618-100  Maltose and Glucose Colorimetric/Fluorometric Assay Kit  100 assays
 K628-100  Maltose Colorimetric/Fluorometric Assay Kit  100 assays
 K776-100  Phosphofructokinase (PFK) Activity Colorimetric Assay Kit  100 assays
 K774-100  Phosphoglucomutase Colorimetric Assay Kit  100 assays
 K775-100  Phosphoglucose Isomerase Colorimetric Assay Kit  100 assays
 K611-100  PicoProbe™ Fructose Fluorometric Assay Kit  100 assays
 K689-100  PicoProbe™ Fructose-6-Phosphate Fluorometric Assay Kit   100 assays
 K688-100  PicoProbe™ Glucose Fluorometric Assay Kit  100 assays
 K751-100  PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)  100 assays
 K687-100  PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit  100 assays
 K769-100  PicoProbe™ Hexokinase Activity Assay Kit (Fluorometric)   100 assays
 K662-100  PicoProbe™ Lactulose Fluorometric Assay Kit   100 assays
 K770-100  PicoProbe™ Phosphoglucomutase Assay Kit (Fluorometric)  100 assays
 K7433-100  ProInsulin (human) ELISA Kit  100 assays
 K679-100  Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit  100 assays
 K566-100  Sialic Acid (NANA) Colorimetric/Fluorometric Assay Kit  100 assays
 K647-100  Starch Colorimetric/Fluorometric Assay Kit  100 assays
 K626-100  Sucrose Colorimetric/Fluorometric Assay Kit  100 assays
 K645-100  Total Carbohydrate Colorimetric Assay Kit  100 assays
 K670-100  Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit  100 assays

 

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

U0126 | MEK選擇性非競爭型抑制劑 貨號1668-5    A MEK inhibitor Abcam操作手冊 (Abcam ab120241)

Alternate Name/Synonyms: 1,4-Diamino-2,3-dicyano-1,4-bis(o-aminophenylmercapto) butadiene

1668_1.gif

同質商品7.jpg

MAP kinase kinase(MEK)選擇性非競爭型抑制劑. Potent and selective non-competitive inhibitor of MAP kinase kinase(MEK). Inhibits MEK-1 and MEK-2 (IC₅₀ values of 0.07 and 0.06 µM respectively) with little or no effect on the activities of PKC, Abl, Raf, MEKK, ERK, JNK, MKK-3, MKK-4/SEK, MKK-6, Cdk2 or Cdk4. 分子型式C₁₈H₁₆NS | 分子量Molecular Weight: 380.48 | 純度Purity: ≥98% by HPLC | Solubility: DMSO (>10 mg/ml)

操作手冊2.jpg

 Catalog #  Product Name+  Size
 1594-100  BAY 43-9006  100 mg
 1594-25  BAY 43-9006  25 mg
 1954-5  Doramapimod  5 mg
 1954-25  Doramapimod  25 mg
 1783-5  EZSolution™ PD 98059  5 mg
 1871-1  EZSolution™ PD184352  1 mg
 1786-1  EZSolution™ SB 203580  1 mg
 2729-5  JNK-IN-8  5 mg
 2729-25  JNK-IN-8  25 mg
 7011-50  L-JNK Peptide Inhibitor I  50 µl
 K862-7  PathwayReady™ MAP Kinase Signaling Inhibitor Panel  7 inhibitors
 1666-5  PD 98059  5 mg
 1643-2  PD-0325901  2 mg
 1643-5  PD-0325901  5 mg
 1643-25  PD-0325901  25 mg
 1585-25  PD184352  25 mg
 1585-5  PD184352  5 mg
 1585-1  PD184352  1 mg
 1712-1  SB 203580  1 mg
 1712-5  SB 203580  5 mg
 1669-5  SP600125  5 mg
 1668-5  U0126  5 mg
 1598-1  VX-702  1 mg
 1598-5  VX-702  5 mg
文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

蛋白質萃取系列-Detergen洗滌劑商品

原理與知識.jpg

 

Detergents洗滌劑是包含polar極性和hydrophobic regions疏水區域的amphipathic molecules兩親性分子,其允許它們作為solubilizing agents增溶劑。洗滌劑可以分為幾個主要類別:離子洗滌劑;非離子洗滌劑和兩性離子洗滌劑。離子洗滌劑具有帶電頭基(陰離子或陽離子),可用於SDS-PAGE期間細胞的完全破壞和蛋白質變性分離。這些可以包括陰離子洗滌劑如SDS,陽離子洗滌劑如CTAP和陰離子膽汁鹽如deoxycholate脫氧膽酸鹽。通常稱為非變性洗滌劑的非離子洗滌劑可用於膜蛋白的溶解,因為它們能夠破壞脂質 - 脂質和脂質 - 蛋白質的相互作用,但具有有限的破壞蛋白質 - 蛋白質相互作用的能力。它們包括低CMC洗滌劑如Triton X-100和不能透析的NP-40替代物,不帶電的膽汁鹽,如Big Chap(可透析)和非常透析的(CMC〜25mM)烷基葡糖苷如辛基吡喃葡萄糖苷。兩性離子洗滌劑保護蛋白質的天然狀態而不改變蛋白質分子的天然電荷。除了溶解性質外,兩性離子洗滌劑對於等電聚焦和2D電泳非常有用。許多合成的兩性離子洗滌劑被稱為磺基甜菜鹼。磺基甜菜鹼在廣泛的pH範圍內保持其兩性離子特徵,並廣泛用於2D凝膠電泳。非洗滌劑磺基甜菜鹼(NDSB)是一類兩性離子化合物,具有類似於兩性離子洗滌劑的親水基團,但具有更短的疏水鏈,不能聚集形成膠束。雖然不被認為是洗滌劑,但是當與洗滌劑一起使用時,NDSB可以提高膜,核和細胞骨架蛋白的產量。結合磺基甜菜鹼基團的電荷中和的短疏水基團導致獲得更高的膜蛋白產率。 NDSB還有助於減少聚集並幫助重折疊包涵體和細菌表達系統中發現的蛋白質。 NDSB在寬pH範圍內是兩性離子的,通過透析容易去除,並且在近紫外範圍內不會顯著吸收。

NP-40 (10% in H₂O) | 貨號2111 (Abcam ab142227)

產品描述:A 10% soultion of NP-40 in water.

Cat # +Size 2111-100
Size 100 ml
Highlights • Alternate Name/Synonyms: 4-Nonylphenyl-polyethylene glycol, Nonidet P™ 40
• Appearance: Liquid
• Formulation: 10% (H₂O)
• CAS Number: N/A
• Molecular Formula: N/A
• Molecular Weight: N/A
• Purity: N/A
• Solubility: N/A

 

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

Home    Literature   Promotions  Product Citations

cAMP分析試劑盒 貨號K371-100   (abcam ab65355)

cAMP Direct Immunoassay Kit (Colorimetric) 

k371.jpg

產品特點2-300x101.jpg

腺苷3',5'-環一磷酸酯(環狀AMP,cAMP)是參與許多生理過程的重要“第二信使”。 BioVision的cAMP分析試劑盒可提供直接競爭性疫分析法,用於靈敏和定量測定生物樣品中的cAMP水平。 該試劑盒利用重組蛋白G包被的96孔板將cAMP多克隆抗體有效地錨定在板上。 cAMP-HRP共軛物與樣品上的cAMP抗體直接競爭,與cAMP競爭。 孵育和洗滌後,可通過讀取OD450 nm處的HRP活性輕鬆確定結合到板上的cAMP-HRP的量。 OD450 nm的強度與樣品中cAMP的濃度成反比。 此外,該試劑盒提供了新的乙酰化步驟,可顯著提高檢測靈敏度。 該試劑盒可檢測〜0.1-10 pmol / 5μl(或〜0.02-2μM)cAMP樣品。  此產品需由儀器進行定量 ELISA儀器設備請按此

同質商品7.jpg

Cat # +Size K371-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications The kit can detect ~0.1-10 pmol/5 μl (or ~ 0.02-2 μM) cAMP samples.
Features & Benefits • Simple procedure
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
• The kit provides a new acetylation procedure to improve detection sensitivity significantly
Kit Components • 10X cAMP Assay Buffer
• Standard cAMP (10 nmol)
• Neutralizing Buffer
• Acetylating Reagent A
• Acetylating Reagent B
• Rabbit Anti-cAMP pAb
• HRP Developer
• Protein G Coated Plate
Storage Conditions -20°C
Shipping Conditions Gel Pack

 

LINE群發(RT-9600).jpg

Ana I. Duarte, et al., (2018) Dual Therapy with Liraglutide and Ghrelin Promotes Brain and Peripheral Energy Metabolism in the R6/2 Mouse Model of Huntington’s Disease, Scientific Reports, Jun. 2018, 29895889

Bo, Zhang et al. (2017) G Protein Alpha S Subunit Promotes Cell Proliferation of Renal Cell Carcinoma with Involvement of Protein Kinase A Signaling, DNA Cell Biol. 2017 Mar;36(3):237-242.

Zou, Shenglong et al. (2017) Somatostatin receptor 5 is a prominent regulator of signaling pathways in cells with coexpression of Cannabinoid receptors 1, Neuroscience. 2017 Jan 6;340:218-231.

Sun, Yan-Yan et al. (2016) Surface expression of hippocampal NMDA GluN2B receptors regulated by fear conditioning determines its contribution to memory consolidation in adult rats Sci Rep. 2016 Aug 4;6:30743. doi: 10.1038/srep30743.

Li and Lytton. An Essential Role for the K+-dependent Na+/Ca2+-exchanger, NCKX4, in Melanocortin-4-receptor-dependent Satiety. J. Biol. Chem., Sep 2014; 289: 25445 - 25459.

相關產品-300x96.jpg

乾浴加熱水浴槽 |  Finnpipette F3 微量分注器 |  試管振盪器 Vortex Mixer  |  Genereach迷你型微量離心機  |   ELISA儀器

 

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

殼聚醣/甲殼素檢測 |  Chitosan Colorimetric Assay Kit 貨號K995-100 (abcam ab273338)

Chitosan Colorimetric Assay Kit

原理與知識.jpg

 

 

殼聚醣/甲殼素 (Chitosan)是D-葡糖胺(D-glucosamine)和N-乙酰葡糖胺(N-acetylglucosamine)的線性陽離子氨基多醣 (cationic amino-polysaccharide)。它可以通過甲殼類動物從甲殼類動物殼中脫乙酰化 (deacetylation of Chitin)而得到,包括蝦(shrimps),龍蝦(lobsters),螃蟹(crabs)等。殼聚醣豐富,無毒,可生物降解,具有生物相容性。它在食品工業(food industry)中的防腐劑 (preservatives),農業工業中的抗微生物劑(antimicrobials),生物技術中的DNA / siRNA納米粒子(DNA/siRNA nanoparticles) ,生物醫學科學中的水凝膠(hydrogels),製藥工業中的藥物輸送(drug delivery)等行業中具有廣泛的應用。此外,殼聚醣 (Chitosan) 的小寡聚物也是如此。研究它們的抗氧化(anti-oxidant),抗炎(anti-inflammatory),免疫增強(immune enhancing),抗腫瘤(anti-tumor,),抗菌特性(anti-bacterial properties)。 BioVision的殼聚醣檢測試劑盒 (BioVision’s Chitosan Assay Kit, k995-100)是第一款用於測量各種樣品中殼聚醣(Chitosan)含量的市售試劑盒。在該測定中,將殼聚醣(Chitosan)轉化為中間體以產生粉紅色產物,然後通過532nm處的吸光度檢測。吸光度信號與殼聚醣濃度成正比。 BioVision的殼聚醣檢測試劑盒快速,靈敏,方便。在測定條件下,它可以檢測低至10μg/ ml。

k995-c.png

 

Cat # +Size K995-100
Size 100 assays
Kit Summary BioVision’s Chitosan Assay Kit is the first commerically available kit to measure the Chitosan content in various samples. In this assay, Chitosan is converted to an intermediate to generate a pink colored product, which is then detected by absorbance at 532 nm. The absorbance signal is directly proportional to the Chitosan concentration. BioVision’s Chitosan Assay Kit is rapid, sensitive and convenient. It can detect as low as 10 μg/ml under assay conditions.
Species Reactivity All
Applications Determination of Chitosan in different solid and liquid samples
Features & Benefits • Rapid
• Sensitive
• Convenient
• It can detect as low as 10 μg/ml under assay conditions
Kit Components • Chitosan Standard
• Chitosan Assay Solution
• Chitosan Converter
• Chitosan Detector
文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

BrdU細胞增殖測定試劑盒 貨號K306-1000 (abcam ab287841)

BrdU Cell Proliferation Assay Kit

原理與知識.jpg

5-溴-2-脫氧尿苷(5-bromo-2-deoxyuridine, BrdU)是嘧啶類似物 ( pyrimidine analog)。 它被 incorporated 到新合成的增殖細胞DNA (proliferating cells) 中,代替胸苷 (thymidine)。 Biovision的BrdU細胞增殖測定試劑盒(BrdU Cell Proliferation Assay Kit, k306) 使用小鼠抗BrdU抗體 (anti-BrdU antibody) 檢測摻入的BrdU。 使用抗小鼠HRP連接的二抗 ( anti-mouse HRP-linked secondary antibody) 來檢測與BrdU結合的抗BrdU抗體,然後加入TMB(HRP substrate)。 顯色程度與摻入細胞中的BrdU量成正比,可以直接用作細胞增殖 (cell proliferation) 的指標。 與其他細胞增殖測定相比,該試劑盒僅檢測增殖細胞而不檢測seeded cells。 這種高度敏感的非放射性試劑盒可檢測到少於50-100個增殖細胞 (proliferating cells)。

 

操作手冊2.jpg

k306.jpg

同質商品7.jpg

Detection Method Absorbance (450 nm)
Species Reactivity N/A
Applications Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients.
Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.
Features & Benefits • Convenient & non-radioactive
• High-throughput suitable
• Highly-sensitive: detect as low as 50-100 proliferating cells.
• Suitable for cell proliferation, chemotaxis and cytotoxicity
Kit Components • BrdU (1000X)
• Fixing/Denaturing Solution
• BrdU Detection Antibody (300X)
• Anti-mouse HRP-linked Antibody (2000X)
• Antibody Diluent
• Wash Buffer (10X)
• TMB Substrate
• Stop Solution

Ye Chen, Bromodomain and extraterminal proteins foster the core transcriptional regulatory programs and confer vulnerability in liposarcoma. Nat Commun., March 2019;  30903020.

Severa Bunda, CIC protein instability contributes to tumorigenesis in glioblastoma. Nat Commun., Feb 2019; 30737375.

Chen, Si-Le et al. (2016) Targeting CRMP‑4 by lentivirus‑mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth, Exp Ther Med. 2016 Oct;12(4):2003-2008.

 

 

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

葡萄糖呈色/螢光分析套組 | Glucose Assay Kit K606-100 (ABCAM ab65333)

 

葡萄糖(C 6 H 12 O 6; FW:180.16)是產生通用能量分子ATP的非常重要的燃料源。 葡萄糖水平是許多代謝紊亂的關鍵診斷參數。 測量葡萄糖在研究和藥物發現過程中都是非常重要的。 葡萄糖測定試劑盒可以在各種生物樣品(例如血清,血漿,體液,食物,生長培養基等)中直接測量葡萄糖。 葡萄糖酶混合物特異性氧化葡萄糖以產生與染料反應產生顏色(λ= 570nm)和螢光(Ex / Em = 535/587nm)的產物。 產生的顏色和螢光與葡萄糖量成正比。 該方法快速,簡單,靈敏,適合高通量。 該測定法也適用於在發酵過程中監測葡萄糖水平和蛋白質表達過程中的葡萄糖供應。 該試劑盒檢測1-10000μM葡萄糖樣品。

k606.jpg

Biovision提供3種葡萄糖濃度試劑盒商品。K606-100:適用於比色和螢光檢測。需要570 nm和Ex / Em:535/587 nm的濾光片. 測量之限制為1-10000 uM。 K686-100僅適用於比色檢測,測量波長為450 nm,測量範圍為20uM至10 mM。葡萄糖的濃度限制不使用氧化或Picoprobe(TM)適用於具有還原物質的樣品。K688-100僅適用於螢光檢測,激發光及散射光設定為Ex / Em:535/587nm。葡萄糖的濃度限制<0.5uM。

商品需用儀器測量  酵素免疫分析儀請按此 (標配405、450、492、630 nm, 可擴增至7個濾片)

 

引用文獻1-300x95.jpg

Meredith A Sommars, Dynamic repression by BCL6 controls the genome-wide liver response to fasting and steatosis. eLife, April 2019;  30983568.
Reka Lorincz, In vivo monitoring of intracellular Ca2+ dynamics in the pancreatic β-cells of zebrafish embryos. Islets, Dec 2018; 30521410.
Ashley R. Maiuri, et al., (2018) Inflammation-induced DNA methylation of DNA polymerase gamma alters the metabolic profile of colon tumors, Cancer Metab, Jul. 2018, 30002826
Singh, Vikash et al. (2017) Salmonella Co-opts Host Cell Chaperone-mediated Autophagy for Intracellular Growth, J Biol Chem. 2017 Feb 3;292(5):1847-1864.
Yasuma, Taro et al. (2016) Amelioration of Diabetes by Protein S Diabetes. 2016 Jul;65(7):1940-51.

同質商品7.jpg

 
文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

鐵降低抗氧化能力(FRAP)檢測試劑盒(比色)| 貨號K515-200  Abcam  ab234626

Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric)

k515-3.jpg

 

 

鐵降低抗氧化能力(Ferric reducing antioxidant power , FRAP)測定法是一種廣泛使用的方法,其在氧化還原連接的比色反應中使用抗氧化劑作為還原劑,其中Fe 3+被還原成Fe 2+。在低pH下鐵(Fe3 +)至亞鐵(Fe2 +)離子的還原導致由無色的鐵 - 探針複合物形成有色的鐵 - 探針複合物。抗氧化劑是通過向自由基提供電子以穩定它們並最小化自由基對DNA,細胞和器官系統造成的損害而充當還原劑的分子。抗氧化劑包括polyphenols多酚等物質; flavonoids黃酮類化合物; vitamins維生素和酶如glutathione peroxidase穀胱甘肽過氧化物酶和superoxide dismutase超氧化物歧化酶。已知它們具有有益的健康效果,例如降低患癌症,心臟病和神經退行性疾​​病的風險,並且在植物,水果,蔬菜,飲料和天然產品中大量存在。 BioVision的FRAP檢測試劑盒為測量各種生物樣品的抗氧化能力提供了一種快速,靈敏且簡便的方法。該測定具有高通量適應性,並且可以檢測低至0.2mM Fe 2+當量的抗氧化能力。

 

操作手冊2.jpg

Cat # +Size K515-200
Size 200 assays
Kit Summary • Detection method- Absorbance (594 nm)
• Species reactivity- Foodstuff, biological fluids, drinks
• Applications- Assay measuring either the combination of both small molecule and protein antioxidants or small molecules antioxidants
Detection Method Absorbance (OD 594 nm)
Species Reactivity Foodstuff, biological fluids, drinks
Applications Assay measuring either the combination of both small molecule and protein antioxidants or small molecules antioxidants
Features & Benefits • Simple procedure; takes less than 2 hours
• Fast and convenient
Kit Components • FRAP Assay Buffer
• FRAP Probe
• FeCl3 Solution
• Ferrous Standard (2 mM)
• FRAP Positive Control
Storage Conditions 4°C
Shipping Conditions Gel Pack

同質商品7.jpg

 

文章標籤

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

1 2345
Close

您尚未登入,將以訪客身份留言。亦可以上方服務帳號登入留言

請輸入暱稱 ( 最多顯示 6 個中文字元 )

請輸入標題 ( 最多顯示 9 個中文字元 )

請輸入內容 ( 最多 140 個中文字元 )

reload

請輸入左方認證碼:

看不懂,換張圖

請輸入驗證碼