太鼎生物科技/太鼎食安科技

DNA Fragmentation相關產品-Red

ApoBrdU
Red DNA Fragmentation Kit

Detects DNA
Fragmentation by FACS or FL
(組織/細胞)

原位觀察DNA Fragmentation (BrdU-Red)，
靈敏度高於DIG方式。三個小時內完成實驗，適用於組織切片或是福馬林固定之細胞，可應用螢光顯微鏡/流氏細胞儀。

產品貨號#k404-60 (包括PC及NC細胞)

Background Descriptions: Internucleosomal DNA fragmentation is a hallmark of apoptosis
in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit
provides complete components including positive and negative control cells for
conveniently detecting DNA fragmentation by fluorescence microscopy or flow
cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate
nucleotides) which is more readily incorporated into DNA strand breaks than
other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater
incorporation gives rise to brighter signal when the Br-dUTP sites are
identified by a fluorescein labeled anti-BrdU monoclonal antibody.

產品介紹

Kit
Summary:

• Detection method- Flow cytometry (Ex/Em = 488/576 nm for BrdU-Red and 488/655
nm for 7-AAD).

• Sample type- Tissue sections and fixed cell preparations

• Species reactivity- Mammalian

• Applications- The TUNEL-based immunohistochemical staining kit provides
complete reagents including positive and negative control slides for convenient
detection of DNA fragmentation

Features
& Benefits:

• Simple procedure; takes ~ 3 hours

• Fast and convenient

• The assay is sensitive and stable

• The TUNEL-based assay kit provides complete components including positive and
negative control cells for convenient detection of DNA fragmentation in
cultured cells and tissue sections.

Kit
components:

• Positive Control Cells

• Negative Control Cells

• Wash Buffer

• Reaction Buffer

• TdT Enzymes

• Br-dUTP

• Rinse Buffer

• Anti-BrdU-Red Mouse IgG1

• 7-AAD/RNase Staining Buffer

Description:


Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian
cells. BioVision’s Apo-BrdU-Red In Situ DNA Fragmentation Assay Kit provides
complete components including positive and negative control cells for
conveniently detecting DNA fragmentation by fluorescence microscopy or flow
cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate
nucleotides) which is more readily incorporated into DNA strand breaks than
other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater
incorporation gives rise to brighter signal when the Br-dUTP sites are
identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The
assay is suitable for studying apoptosis with GFP transfected cells.

Storage
Conditions:

-20°C

Shipping
Conditions:

gel pack

USAGE:
For Research Use Only! Not For Use in Humans.

【連絡人】許虹宜 0920-312382 Hung-Yi Hsu

【E-mail】taiding.biotech@msa.hinet.net

【公司電話】02-86609496【公司傳真】02-86609342

【相關網址】www.biopioneer.com

【公司網址】www.biopioneer.com.tw