太鼎生物科技/太鼎食安科技

DNA Fragmentation相關產品-flow

ApoDIRECT
DNA Fragmentation Assay Kit

Detects DNA Fragmentation by FACS or
FL (細胞)

一個小時內完成。DNA Fragmentation (FITC-dUTP)適用於懸浮型或貼附型細胞，可應用螢光顯微鏡或Flow。

產品貨號#k402-50 (包括PC及NC細胞)

Background
Descriptions: Internucleosomal DNA fragmentation is a hallmark of apoptosis
in mammalian cells. BioVision’s ApoDIRECT In Situ DNA Fragmentation Assay Kit
provides complete components including positive and negative control cells for
conveniently detecting DNA fragmentation by fluorescence microscopy or flow
cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl
transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free
3’-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be
observed by fluorescence microscopy or analyzed by flow cytometry

產品介紹

Kit
Summary:

• Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm
for PI) and fluorescence microscopy (FITC and rhodamine filters)

• Sample type- Cell culture (adherent and suspension)

• Species reactivity- Mammalian

• Applications- The TUNEL-based assay kit provides complete components
including positive and negative control cells for convenient detection of DNA
fragmentation in cultured cells and tissue sections.

Features
& Benefits:

• Simple procedure; takes ~ 1 hour

• Fast and convenient

• The assay is sensitive and stable

• The assay offers an one-step labeling of apoptotic cells.

Kit
components:

• Positive Control Cells

• Negative Control Cells

• Wash Buffer

• Reaction Buffer

• TdT Enzymes

• FITC-dUTP

• Rinse Buffer

• PI/RNase Staining Buffer

DNA Fragmentation相關產品連結