【ApoBrdU DNA Fragmentation Assay Kit】Biovision#k401-60靈敏度高於DIG方式。適用於懸浮或貼附型細胞，可應用螢光顯微鏡/流氏細胞儀。－太鼎生物科技/太鼎食安科技

DNA Fragmentation試劑套組-FITC

ApoBrdU
DNA Fragmentation Assay Kit

Detects DNA
Fragmentation by FACS or FL (細胞)

原位觀察DNA Fragmentation (Brdu-FITC單株抗體)，
靈敏度高於DIG方式。一個小時完成實驗，適用於懸浮或貼附型細胞，可應用螢光顯微鏡/流氏細胞儀。

Background Descriptions: Internucleosomal DNA fragmentation is a hallmark of apoptosis
in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit
provides complete components including positive and negative control cells for
conveniently detecting DNA fragmentation by fluorescence microscopy or flow
cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate
nucleotides) which is more readily incorporated into DNA strand breaks than
other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater
incorporation gives rise to brighter signal when the Br-dUTP sites are
identified by a fluorescein labeled anti-BrdU monoclonal antibody.

產品貨號#k401-60(包括PC及NC細胞)

產品介紹

•
Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm
for PI) and fluorescence microscopy (FITC and rhodamine filters)

• Sample type- Cell culture (adherent and suspension), parafin embedded tissue
sections

• Species reactivity- Mammalian

• Applications- The TUNEL-based assay kit provides complete components
including positive and negative control cells for convenient detection of DNA
fragmentation in cultured cells and tissue sections.

Features
& Benefits:

• Simple procedure; takes ~ 1 hour

• Fast and convenient

• The assay is sensitive and stable

• The incorporation of BrdU in the procedure provides greater sensitivity than
biotinylated or digoxigenylated methods.

Kit
components:

• Positive Control Cells

• Negative Control Cells

• Wash Buffer

• Reaction Buffer

• TdT Enzymes

• Br-dUTP

• Rinse Buffer

• Anti-BrdU-FITC Antibody

• PI/RNase Staining Buffer

DNA Fragmentation相關產品連結

Catalog#	Product Name	Size
3618-100	BrdU單株抗體, 100ug Anti-BrdU antibody (Clone B1G5) 資料整理如下所示 http://tw.myblog.yahoo.com/taiding2000/article?mid=1150&prev=1151&next=1149	100 µg
K401-60	BrdU分析套組 (BrdU-FITC抗體), 60個分析(附對照細胞) 適用懸浮型及貼附型細胞。Flow、顯微鏡螢光觀察。 ApoBrdU DNA Fragmentation Assay Kit 資料整理如下所示 http://tw.myblog.yahoo.com/taiding2000/article?mid=1151&prev=1152&next=1150	60 assays
K404-60	BrdU分析套組 (BrdU-Red抗體), 60個分析(附對照細胞) 適用組織切片或福馬林固定之細胞。Flow、顯微鏡螢光 ApoBrdU Red DNA Fragmentation Kit 資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1152&prev=-1&next=1151	60 assays
K403-50	BrdU分析套組 (BrdU-Biotin抗體), 50個分析(附對照細胞) 適用組織切片。顯微鏡觀察 ApoBrdU-IHC DNA Fragmentation Assay Kit 資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1153&prev=1154&next=1152	50 assays
K402-50	BrdU分析套組 (FITC-dUTP), 50個分析(附對照細胞) 適用懸浮型及貼附型細胞。螢光顯微鏡觀察或Flow ApoDIRECT DNA Fragmentation Assay Kit 資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1154&prev=-1&next=1153	50 assays