Anti-BrdU
antibody (Clone B1G5)


BrdU monoclonal Ab for studying cell
proliferation, cytokinetics etc.





產品貨號#3518-100





Background Descriptions: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd)
is a synthetic nucleoside that is an analogue of thymidine. The
immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA
is a powerful tool to study the cytokinetics of normal and neoplastic cells. In
vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and
the subsequent detection of incorporated BrdU with specific anti-BrdU
monoclonal antibodies is an accurate and comprehensive method to quantitate the
degree of DNA-synthesis. BrdU is incorporated into the newly synthesized DNA of
S-phase cells may provide an estimate for the fraction of cells in S-phase.
Also dynamic proliferative information such as the S-phase transit rate and the
potential doubling time can be obtained, by means of bivariate BrdU/DNA flow
cytometric analysis. Antibody specific for BrdU is used to immunocytochemically
detect the incorporated BrdU, which is a powerful tool to study cell
proliferation, the cytokinetics of normal and neoplastic cells, etc.





Anti-Bromodeoxyuridine (BRDU)
Antibody





溴脫氧尿甘-單多株抗體





Product
Name:
Anti-BrdU antibody (Clone B1G5)





Antibody
Target:
BrdU





Alternative
Name:
BrdU, BUdR, BrdUrd





Host:
Mouse





Antibody
Type:
Monoclonal





Isotype:
Mouse
IgG





Immunogen:
BrdU
covalently coupled to BSA





Accession#:
N/A





Gene
ID:
N/A





Appearance:
Colorless
liquid





Concentration:
0.5
mg/ml





Formulation:
100
µg (0.5 mg/ml) in 10% glycerol in PBS.





Purification:
Protein
A purified





Species
Reactivity:
All species





Positive
Control:
N/A





Application:
Immunohistochemistry,
Immunoprecipitation, Immunocytochemistry, ELISA, Immunofluorescence, FACS





Application
& Usage:
The antibody can be used in IHC, IP, ICC, ELISA, IF, FACS.
However, the optimal concentrations should be determined individually.





 




DNA Fragmentation相關產品連結


 
















































 Catalog# 




 Product Name 




 Size 




 




 




 3618-100 




 BrdU單株抗體, 100ug



Anti-BrdU
antibody (Clone B1G5) 
  



資料整理如下所示



http://tw.myblog.yahoo.com/taiding2000/article?mid=1150&prev=1151&next=1149




 100 µg 




 




 




 K401-60 




 BrdU分析套組 (BrdU-FITC抗體), 60個分析(附對照細胞)



適用懸浮型及貼附型細胞。Flow、顯微鏡螢光觀察。



ApoBrdU
DNA Fragmentation Assay Kit
  



資料整理如下所示



http://tw.myblog.yahoo.com/taiding2000/article?mid=1151&prev=1152&next=1150




 60 assays 




 




 




 K404-60 




BrdU分析套組 (BrdU-Red抗體), 60個分析(附對照細胞)



適用組織切片或福馬林固定之細胞。Flow、顯微鏡螢光 



ApoBrdU
Red DNA Fragmentation Kit
  



資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1152&prev=-1&next=1151




 60 assays 




 




 




 K403-50 




BrdU分析套組 (BrdU-Biotin抗體), 50個分析(附對照細胞)



適用組織切片。顯微鏡觀察 



ApoBrdU-IHC
DNA Fragmentation Assay Kit
  



資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1153&prev=1154&next=1152




 50 assays 




 




 




 K402-50 




BrdU分析套組 (FITC-dUTP), 50個分析(附對照細胞)



適用懸浮型及貼附型細胞。螢光顯微鏡觀察或Flow



ApoDIRECT
DNA Fragmentation Assay Kit
  



資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1154&prev=-1&next=1153




 50 assays 




 




 





 




 


 


 


 


【連絡人】許虹宜 0920-312382
Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司】02-86609496【傳真】02-86609342




【相關網址】
www.biopioneer.com.tw


 


 

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