【HMGB1人類重組蛋白】訂購貨號 # PRO-581。ProSpec重組蛋白活性高，其純度高於98％以上，並經由三種測定方式RP-HPLC、FPLC及SDS-PAGE來分析其純度 @ 太鼎生物科技/太鼎食安科技

HMGB1人類重組蛋白,Human

HMGB1 Human重組蛋白 ,訂購貨號 # PRO-581

產品特色

ProSpec重組蛋白活性高，其純度高於98％以上，並經由三種測定方式RP-HPLC、FPLC及SDS-PAGE來分析其純度，ProSpec生產之重組蛋白其內毒素小於0.1ng/ug，可進行活體實驗。ProSpec產品種類超過千種，產品涵蓋；生長激素(Cytokines), 趨化因子(Chemokines), 生長因子(Growth Fectors), 荷爾蒙(Hormones), Viral
Antigens和各式抗體(antibody)

【ProSpec介紹】

ProSpec公司具有專業生產Recombinant Protein近二十年的經驗，其生產技術具有高產能、及高產率，因而生產成本低，ProSpec重組蛋白在市場上具有相當之競爭性。

Introduction	High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
Description	HMG1 Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 223 amino acids and having a molecular mass of 26 kDa. The HMGB-1 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	The HMG1 (1 mg/ml) was lyophilized after extensive dialyses against 1x PBS pH-7.4.
Solubility	It is recommended to reconstitute the lyophilized HMGB1 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized HMGB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HMGB1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino acid sequence	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKT MSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFF LFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKY EKDIAAYRAKGKPDAAKKGVVKAEKSKKKEEEEDEEDEEDEEEEEDEEDEDE EEDDDDELEHHHHHH.