HMGB1人類重組蛋白,Human





HMGB1 Human重組蛋白  ,訂購貨號 # PRO-581





產品特色





ProSpec重組蛋白活性高,其純度高於98%以上,並經由三種測定方式RP-HPLCFPLCSDS-PAGE來分析其純度,ProSpec生產之重組蛋白其內毒素小於0.1ng/ug,可進行活體實驗。ProSpec產品種類超過千種,產品涵蓋;生長激素(Cytokines), 趨化因子(Chemokines), 生長因子(Growth Fectors), 荷爾蒙(Hormones), Viral
Antigens
和各式抗體(antibody)





ProSpec介紹】





ProSpec公司具有專業生產Recombinant Protein近二十年的經驗,其生產技術具有高產能、及高產率,因而生產成本低,ProSpec重組蛋白在市場上具有相當之競爭性。











































Introduction




High-mobility group box 1 protein (HMGB1),
previously known as HMG-1 or amphoterin, is a member of the high mobility
group box family of non-histone chromosomal proteins. Human HMGB1 is
expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide
containing three domains: two N-terminal globular, 70 aa positively charged
DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa
C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and
178 - 184 contain a NLS. Posttranslational modifications of the molecule have
been reported, with acetylation occurring on as many as 17 lysine residues.
HMGB1 is expressed at high levels in almost all cells. It was originally
discovered as a nuclear protein that could bend DNA. Such bending stabilizes
nucleosome formation and regulates the expression of select genes upon
recruitment by DNA binding proteins.




Description




HMG1 Human Recombinant fused with 6X His tag
produced in E.Coli is a single, non-glycosylated, polypeptide chain
containing 223 amino acids and having a molecular mass of 26 kDa.

The HMGB-1 is purified by proprietary chromatographic techniques.




Source




Escherichia Coli.




Physical Appearance




Sterile Filtered White lyophilized (freeze-dried)
powder.




Formulation




The HMG1 (1 mg/ml) was lyophilized after extensive
dialyses against 1x PBS pH-7.4.




Solubility




It is recommended to reconstitute the lyophilized
HMGB1 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be
further diluted to other aqueous solutions.




Stability




Lyophilized HMGB1 although stable at room
temperature for 3 weeks, should be stored desiccated below -18°C. Upon
reconstitution HMGB1 should be stored at 4°C between 2-7 days and for future
use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or
BSA).

Please prevent freeze-thaw cycles.




Purity




Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.




Amino acid sequence




MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKT

MSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFF

LFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKY

EKDIAAYRAKGKPDAAKKGVVKAEKSKKKEEEEDEEDEEDEEEEEDEEDEDE

EEDDDDELEHHHHHH.






 





ProSpec重組蛋白應用】





廣泛應用於研發、診斷試劑之開發、組織培養或無血清培養基之添加,癌症、細胞凋零、發育、內分泌、免疫、神經科學、蛋白質體、及幹細胞等相關領域的研究。





ProSpec重組蛋白貯存】





產品均依冷凍乾燥方式供應,易於處理與儲存。





【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜0920-312382



【連絡信箱】taiding.biotech@msa.hinet.net



【臺北公司】02-86609496【台中公司】0935-229803 陳俊豪



【公司網址】 www.biopioneer.com.tw


 


 

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