E. coli O157:H7酵素免疫分析套組
E. coli O157:H7 ELISA
Test Kit has the capacity for 96 determinations or testing of 46 samples in
duplicate (assuming 4 wells for positive and negative controls). Most of the
kit components can be stored at 2-8C except for the E. coli O157:H7
controls and 50X Ab#2 stock which should be stored at -20C. Return any unused
microwells to the foil bag and reseal them with the desiccant provided in the
original package. The shelf life is 12 months when the kit is properly stored.
MaxSignal® E. coli O157:H7 ELISA Test Kit is
designed for routine screening for E. coli O157:H7 from cultured food
and feed samples. E. coli serotype O157:H7 is a gram-negative rod-shaped
bacterium. The letter "O" in the name refers to the somatic antigen
number, whereas the "H" refers to the flagella antigen. This is one
of hundreds of serotypes of the bacterium E. coli. While most strains
are harmless and normally found in the intestines of mammals, this O157:H7
strain may produce Shiga-like toxins, cause severe illness, and is a member of
a class of pathogenic E. coli known as enterohemorrhagic E. coli or
EHEC. People infected with enterohemorrhagic E. coli often develop
hemorrhagic colitis and/or hemolytic-uremic syndrome. The sources of
contamination include raw ground beef, unpasteurized milk, untreated water, juice,
raw sprouts, lettuce, and salami.
The detection of E. coli O157:H7 in food samples is often
laborious and time-consuming. Several days are required to culture the bacteria
from a sample followed by a final isolation and identification by biochemical
and serological techniques. The MaxSignal® E. coli O157:H7 ELISA Test
Kit provides a simple, rapid, sensitive and cost-effective enzyme
immunoassay (ELISA) screening method, which enables government agencies,
seafood processors, as well as quality assurance organizations, to detect
positive and negative E. coli O157:H7 samples to as low as 105 cells/mL
The method is based on a
colorimetric ELISA assay. The monoclonal antibody against E. coli O157:H7
has been coated in the plate wells. During the analysis, the sample is added.
If E. coli O157:H7 is present in the sample, it will bind with the
antibody. The secondary antibody, tagged with a peroxidase enzyme, targets the E.
coli O157:H7 that is complexed to the primary antibody coated on the plate
wells. The resulting color intensity, after addition of substrate, is related
to the amount of E. coli O157:H7 in the sample.
kit can detect 105cells/mL.
kit can broadly detect E. coli O157:H7 antigen.
Materials Not Provided With the Kit
plate reader (450 nm)
mixer, (e.g. Geneie Vortex mixer from VWR)
§ 10, 20,
100 and 1000 L pipettes
pipette: 50-300 L (Optional)
§ Modified TSB Broth (mTSB): Consisting
of Trypticase soy broth (TSB) supplemented with Casamino Acids (10 g/liter) and
K2HPO4 (1.5 g/liter).
Food and Feed Safety Products