Tyrosinase Assay Kit (Fluorometric) | abnova 貨號KA6032
靈敏度6.25 to 400 U/mL
酪氨酸酶(Tyrosinase)是一種在從細菌(bacteria )和真菌(fungi)到動物的廣泛物種中表達的酶。酪氨酸酶(Tyrosinase)對藥物發現、生命科學研究、食品工業和化妝品工業具有重要意義。因為它在黑色素(melanin)的生物合成途徑( biosynthetic pathway )中起著重要作用。酪氨酸酶抑製劑的開發和篩選在治療黑色素瘤(melanoma)相關疾病方面受到了極大關注。酪氨酸酶(Tyrosinase)水平和活性在黑色素瘤(melanoma)被認為是監測黑色素瘤( melanoma)相關的可靠測試疾病。酪氨酸酶檢測試劑盒(The Tyrosinase Assay Kit, 螢光法)是一種簡單、一步且可靠的檢測酪氨酸酶( tyrosinase activity )的方法,具高靈敏度。該測定使用專有的螢光底物,顯著在與酪氨酸酶反應後增加其在 440 nm 處的螢光強度。螢光增加440 nm 處的強度與酪氨酸酶活性密切相關。
- Product Description:
- Tyrosinase Assay Kit (Fluorometric) is a simple, one-step and reliable assay for monitoring tyrosinase activity with very high sensitivity. The assay uses a proprietary fluorogenic substrate that significantly increases its fluorescence intensity at 440 nm upon reaction with tyrosinase. The increases in fluorescence intensity at 440 nm is well correlated with tyrosinase activity. The assay kit is designed to be run with a fluorescence microplate reader.
- Sample Volume:
- 50 uL
- Detection Method:
- Fluorimetric
- Calibration Range:
- 6.25 to 400 U/mL
- Regulatory Status:
- For research use only (RUO)
- Quality Control Testing:
- Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
- Storage Instruction:
- Store the kit at -20°C and avoid from light.