DAPI 藍色螢光螢劑，是一種能與DNA強力結合的螢光染劑。當染劑結合至dsDNA AT regions時，螢光會呈現20倍強度。最大吸收波長為364nm/散射波長454nm (藍色)。DAPI常用於顯微鏡觀察，螢光染劑可以透過完整的細胞膜，可用於活細胞 (高濃度時)和固細胞的染色。
DAPI (4',6-diamidino-2-phenylindole) is used extensively as a nuclear stain that binds strongly to A-T rich regions in the minor groove of double-stranded DNA. The blue fluorescence increases 20-fold when DAPI binds to dsDNA. Since it can pass through cell membrane, DAPI can be used to stain both live and fixed cells, but much higher concentration has to be used for live cells as its permeability for live cell membrane is dramatically less.
DNAfectin™ 2100 Transfection Reagent is a unique formulation of multiple polycations and liposomes that enable highly efficient and effective DNA transfection of eukaryotic cells. DNAfectin™ is the best transfection reagent for over 15 different cell lines, including difficult-to-transfect, suspension, and primary cells. This transfection reagent is perfect for any of your transfection needs.
Application
Transfection of DNA into cultured eukaryotic cells.
Protocol
Kit Contents
1ml at 1.0mg/1.0ml
Storage
Store at 4ºC. Do not freeze.
Notes
Please check our Transfection Cell Database for tested cell lines
This product contains 250 µg/ml of amphotericin B and 205 µg/ml of deoxycholic acid in normal saline. It is 0.2 micron filtered and is tested for sterility.
Storage
This product is stored at -20°C . At 37°C , it is stable for up to 7 days. At 2 -8°C , it is stable for up to 3 weeks. Avoid freeze-thaw cycles.
Data Sheet
Product Description
This product contains 250 μg/ml of amphotericin B and 205 μg/ml of deoxycholic acid in normal saline. It is 0.2 micron filtered and is tested for sterility.
Usage
Amphotericin B is an antifungal agent used in cell cultures. It should be added to cell cultures at a maximum concentration of 10ml per litre of medium.
Precautions and Disclaimer
For laboratory use only. Not for drug, household or other uses.
Storage
This product is stored at -20oC. At 37oC, it is stable for up to 7 days. At 2-8oC, it is stable for up to 3 weeks. Avoid freeze-thaw cycles.
This product contains 50 mg/ml of active G418. It is 0.2 micron filtered and is tested for sterility.
Storage
This product is stored at 2 -8°C and is stable for 2 years.
Data Sheet
Product Description
This product contains 50 mg/ml of active G418. It is 0.2 micron filtered and is tested for sterility.
Usage
The aminoglycoside antibiotic G418 is effective against both eukaryotic and prokaryotic cells. It is used to select for cells that express neomycin resistance as a result of successful transfection. G418 should be added to animal cell cultures at a concentration of 400μg/ml for selection purposes, while a concentration of 200μg/ml is sufficient for maintenance. For prokaryotic cells, the maximum concentration is 5μg/ml.
Precautions and Disclaimer
For laboratory use only. Not for drug, household or other uses.
Storage
This product is stored at 2-8oC and is stable for 2 years.
Penicillin- Streptomycin contains 10,000 units of penicillin (base), 10,000µg of streptomycin (base) per mL in WFI water. It is supplied as a 0.22 µm-filtered, 100X frozen liquid.
Storage
This product is stable for 2 years at -20°C .
Data Sheet
Product Description
Penicillin- Streptomycin contains10,000 units of penicillin (base), 10,000 μg of streptomycin (base) per mL in WFI water. It is supplied as a 0.22 μm-filtered, 100X frozen liquid.
Usage
As a 100X concentration, it is designed to be supplemented as 1 ml per 99 ml of basal medium, yielding an iso-osmotic solution at final dilution.
This product contains 50 mg/ml of active gentamicin sulfate in WFI water. It is 0.2 micron filtered and is tested for sterility.
Storage
This product is stored at 2 -8°C . At room temperature, this product should be consumed within two days. It is stable at 37oC for five days.
Data Sheet
Product Description
This product contains 50 mg/ml of active gentamicin sulfate in WFI water. It is 0.2 micron filtered and is tested for sterility.
Usage
Gentamicin has the advantage of being a broad-spectrum antibiotic that is non-toxic to animal cells and viruses at recommended concentrations. It should be added to cell cultures at a maximum concentration of 1ml per litre, with a working concentration of 50 μg/mL for eukaryotic cell culture and 15 μg/mL for prokaryotic cell culture.
Precautions and Disclaimer
For laboratory use only. Not for drug, household or other uses.
Storage
This product is stored at 2-8oC. At room temperature, this product should be consumed within two days. It is stable at 37oC for five days.
The USDA research grade origin fetal bovine serum is an economical alternative to more stringently purified serums. It contains a maximum endotoxin level of 20EU/ml and a maximum hemoglobin level of 30mg/dl. This product is best suited for routine cell culture work.
Sterility
This USP sterility tested product has been triple 0.1µm filtered. It has been screened for mycoplasma and viruses, and is certified to be free of bovine diarrhea virus, cytopathogenic agents, and hemoadsorbing agents.
Storage
This product is stored at -20°C , with a shelf life is 60 months. Repeated freeze-thaw cycles should be avoided by making aliquots upon first thawing. Like most serum products, turbidity may occur upon thawing. This does not affect the performance of this product.
Data Sheet
USDA Dialyzed Fetal Bovine Serum
Cat.No.:
TM997-500
Quantity:
500ml
Price:
請電洽02-86609496
Description
The USDA dialyzed fetal bovine serum is a highly purified serum designed for the investigation of alternative biochemical pathways. The levels of small molecules such as amino acids and nucleosides have been minimized using a dia-filtration process. This product contains a maximum endotoxin level of 10EU/ml and a maximum hemoglobin level of 20mg/dl.
Sterility
This USP sterility tested product has been dialyzed using a 10,000MW cutoff filter against normal saline. The concentrations of hypoxanthine and thymidine have been reduced below detectable levels. The level of glucose, which is indicative of the extent of small molecule removal, has a maximum of 5mg/dl. This product has also been screened for mycoplasma and viruses, and it is certified to be free of bluetongue virus, bovine adenovirus, bovine parvovirus, bovine respiratory syncytial virus, bovine diarrhea virus, rabies virus, reovirus, cytopathogenic agents, and hemoadsorbing agents.
Storage
This product is stored at -20°C , with a shelf life is 60 months. Repeated freeze-thaw cycles should be avoided by making aliquots upon first thawing. Like most serum products, turbidity may occur upon thawing. This does not affect the performance of this product.
Data Sheet
USDA Charcoal Stripped Fetal Bovine Serum
Cat.No.:
TM998-500
Quantity:
500ml
Price:
請電洽02-86609496
The USDA charcoal stripped fetal bovine serum is a highly purified serum designed for specialized cell culture work. The levels of hormones, growth factors, and steroids have been minimized, making this product ideal for the study of cell signalling. This product contains a maximum endotoxin level of 10EU/ml and a maximum hemoglobin level of 20mg/dl.
Sterility
This USP sterility tested product has been triple 0.1µm filtered. It has been screened for mycoplasma and viruses, and is certified to be free of bluetongue virus, bovine adenovirus, bovine parvovirus, bovine respiratory syncytial virus, bovine diarrhea virus, rabies virus, reovirus, cytopathogenic agents, and hemoadsorbing agents.
Storage
This product is stored at -20°C , with a shelf life is 60 months. Repeated freeze-thaw cycles should be avoided by making aliquots upon first thawing. Like most serum products, turbidity may occur upon thawing. This does not affect the performance of this product.
In cell biology, processes such as cell differentiation, cell cycle and cell survival are affected by signals stimulated through cell adhesion. Therefore adhesion plays a fundamental role in in vitro studies. ABM's Applied Cell Extracellular Matrix composes mainly of type I collagen, isolated from rat tail tendon. Type I collagen is the most abundant form of collagen and differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. This product is suitable for usage as a solid gel or for coating of culture wares. ABM's Applied Cell Extracellular matrix on culture surfaces have been shown to enhance cell attachment in a wide variety of cell lines such as hepatocytes, fibroblasts and epithelial cells.
Features
-sterile liquid containing 4mg/ml of pure collagen (>95%).
-stored in 20 mM acetic acid (pH ~3.0).
-direct addition to culture wares for coating
-simple and rapid plate coating procedure.
-enhance cell attachment in a variety of primary cells.
In cell biology, processes such as cell differentiation, cell cycle and cell survival are affected by signals stimulated through cell adhesion. Therefore adhesion plays a fundamental role in in vitro studies. ABM's Applied Cell Extracellular Matrix composes mainly of type I collagen, isolated from rat tail tendon. Type I collagen is the most abundant form of collagen and differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. This product is suitable for usage as a solid gel or for coating of culture wares. ABM's Applied Cell Extracellular matrix on culture surfaces have been shown to enhance cell attachment in a wide variety of cell lines such as hepatocytes, fibroblasts and epithelial cells.
Features
-sterile liquid containing 4mg/ml of pure collagen (>95%).
-stored in 20 mM acetic acid (pH ~3.0).
-direct addition to culture wares for coating
-simple and rapid plate coating procedure.
-enhance cell attachment in a variety of primary cells.
This product contains 29.2 g /L of L-glutamine in normal saline. It is 0.2 micron filtered and is tested for sterility. While L-glutamine is most stable at 4oC and neutral pH, it can spontaneously degrade even under these conditions. L-glutamine should be kept at low temperatures to minimize the formation of ammonia as a degradation product.
Uses
The essential amino acid L-glutamine is used in energy production, protein synthesis, and nucleic acid generation by most animal and insect cells. This product can be used as a nutrient supplement in cell cultures.
Precautions and Disclaimer
For laboratory use only. Not for drug, household or other uses.
Storage
This product is stored at -20oC, with a shelf life of two years. Once thawed, the product should be used within two weeks.
Cat.No.:
G275
Quantity:
100ml
Price:
請電洽02-86609496
Description
This product contains 29.2 g /L of L-glutamine in normal saline. It is 0.2 micron filtered and is tested for sterility. While L-glutamine is most stable at 4oC and neutral pH, it can spontaneously degrade even under these conditions. L-glutamine should be kept at low temperatures to minimize the formation of ammonia as a degradation product.
Storage
This product is stored at -20°C , with a shelf life of two years. Once thawed, the product should be used within two weeks.
Trypsin-EDTA (0.25%) contains 2.5 g /L Porcine Trypsin (tested for porcine parvovirus) and 0.2g /L EDTA-Na in Hanks Balanced Salt Solution, with Phenol Red and without Ca2+ and Mg 2+.
Storage
This product is stable for 2 years at -20°C .
Data Sheet
Trypsin-EDTA 0.25% Solution
Store at -20°C
Product Description
Trypsin-EDTA (0.25%) contains 2.5 g /L Porcine Trypsin (tested for porcine parvovirus) and 0.2g /L EDTA•4Na in Hanks’ Balanced Salt Solution, with Phenol Red and without Ca2+ and Mg 2+.
Usage
Trypsin-EDTA (0.25%) is recommended for detachment of adherent cells, enzymatic dissociation of tissue into single cell suspensions, dissociation of ES cell colonies, and dissociation of embryoid bodies.
Precautions and Disclaimer
For laboratory use only. Not for drug, household or other uses.
• 使用前請將“T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ”回溫至室溫。
• 在使用“T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ”前請盡量去除樣品上殘留之液體。
• “T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ”適用於大多數樣品於差異干擾差、相位差或螢光顯微鏡下觀察。
操作：
1.回溫試劑：
將“T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ”自4oC 冰箱中取出並回溫至室溫備用。請勿將“T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ”以溫水或其他加熱方式回溫，以免破壞產品穩定性。
2.使用Apply T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ：
在使用“T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ”前請將樣品玻片上之殘留液體以拭鏡紙由玻片邊緣輕輕去除並在玻片上滴上一滴“T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ”（10-20ul）。將樣品蓋玻片以45度角，緩慢的蓋在含有“T-Pro Mounting Reagent or T-Pro Mounting Long Reagent ”之載玻片上，其間請避免氣泡之產生。
圖1. T-Pro Mounting Long Reagent I 有幫助螢光分子抵抗光漂白之效果。圖1.a NIH 3T3細胞以4％福馬林固定後再以Fluorescein-phalloidin對細胞骨架進行染色。 圖中影像乃是將染色完成之樣品置於T-Pro Mounting Long Reagent I或PBS中，並在封片後以100瓦汞燈對樣品進行不同時間之照射以產生光漂白之效果，再以63x/1.4 NA 油鏡及16-bit 單色 CCD攝影機在相同條件下進行拍攝。在所得影像中可清楚得知於PBS對照組中，在螢光照射120秒以後，絕大部分之螢光分子因光漂白而無法呈色，而在實驗組中發現T-Pro Mounting Long Reagent I能幫助螢光分子抵抗光漂白，直至螢光照射7分鐘後，大部分之螢光分子才因光漂白而無法呈色，因此說明了T-Pro Mounting Long Reagent I有幫助螢光分子抵抗光漂白之效果。並將兩組實驗在不同時間所取得之影像，以軟體對螢光衰減率進行分析後所做之圖。
下圖、以T-Pro Mounting Long Reagent I with DAPI、Alexa Fluo 488 phalloidin及Alexa Fluo 594 wheat germ agglutinin對NIH 3T3細胞進行染色之成果。
Escpecially developed for the efficient transfection of eukaryotic cells with RNAi oligo's. This transfection reagent is perfect for any of your RNAi transfection needs.
Application
RNAi knockdown experiments in a wide variety of cell lines (HeLa, HEK-293 etc). Highly specific and non-toxic transfection.
Protocol
Storage
Store at 4ºC. Do not freeze.
Notes
This product is distributed for laboratory research only. Caution: Not for diagnostic use .
產品介紹 Description
ANAifectin™ is a transfection reagent specially formulated with multiple cationic polymers. It is suitable for the transfection of RNAi oligoes into cultured eukaryotic cells.
RNAi轉染操件手冊:
These conditions are recommended as guidelinesonly. A 6-well or 35mm dish is adequate for most applications, but larger vessels are sometimes required. In that case, consult table 1.
1. In a 6-well plate, seed cells at a density of 1-3x10⁵ per well in 2.0ml of the appropriate growth medium (with serum if cells are cultured in presence of serum). Incubate the cells at 37°C until cells are 60-90% confluent. This will usually take 18-24 hours, depending on cell types. Since transfection efficiency is sensitive to culture confluence, it is important to maintain a standard seeding protocol from experiment to experiment
2. Prepare the following solutions in sterile 12x75 mm or microcentrifuge tubes: Solution A: For each transfection, dilute 1-3μg of RNAi oligoes into 125μl serum-free medium. Solution B: For each transfection, dilute 4-10μl of RNAifectin™ reagent into 125μl of serum-free medium. (Vortex RNAifectin™ reagent before use)
3. Combine the solutions, mix thoroughly and incubate at room temperature for 20 mins to allow DNA-lipsome complexes to form.
4. Remove growth medium from the cells and add 800μl of serum-free medium to the cells.
5. Add the RNAifectin™-oligo complexes to thecells, mix gently to ensure uniform distribution and incubate at 37°C for 2-24 hours. We recommend starting with 5 hours.
6. Remove the transfection solution and add 2.0ml of the appropriate complete growth medium(with serum) to each well. Incubate cells at 37°Cfor a total of 24-72 hours.
7. Assay cell extracts for gene activity 24-72 hours aer the start of transfection depending on the cell types and promoter activity.
8. A similar procedure can be used to transfect an RNAi vector for stable expression. At 72 hours aer transfection, split the cells at a ratio of 1:10 into the selective medium for the marker gene transfected.
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