A型流感WSN/1933--HA重組蛋白質
Influenza A H1N1
( A/WSN/1933 ) HA / Hemagglutinin Protein
H1N1
( A/WSN/1933 ) HA Protein Product Information
Synonym :
|
HA
|
Protein Construction:
|
A DNA sequence encoding the
extracellular domain of the influenza A H1N1 (A/WSN/1933 (H1N1))
hemagglutinin (ACF54598.1) ( Met 1-Gln 528, HA1+HA2, uncleved ), was fused
with a C-terminal polyhistidine tag
|
Source:
|
Influenza A H1N1
|
Expression Host:
|
Human Cells
|
H1N1
( A/WSN/1933 ) HA Protein QC Testing
Purity:
|
> 97 % as determined by SDS-PAGE
|
SDS-PAGE:
H1N 1 HA protein
|
Endotoxin:
|
< 1.0 EU per μg of the protein as
determined by the LAL method.
|
Stability:
|
Samples are stable for up to twelve
months from date of receipt at -70℃
|
Predicted N terminal:
|
Asp 18
|
Molecular Mass:
|
The secreted recombinant influenza A
H1N1 hemagglutinin (A/WSN/1933 (H1N1)) comprises 522 amino acids and has a
predicted molecular mass of 59 kDa. As a result of glycosylation, the
apparent molecular mass of the protein is approximately 60-70 kDa in SDS-PAGE
under reducing conditions
|
Formulation:
|
Lyophilized from sterile PBS , pH 7.4
- Normally 5 % - 8 % trehalose and mannitol are added as protectants
before lyophilization. Specific concentrations are included in the
hardcopy of COA.
- Please contact us
for any concerns or special requirements.
|
H1N1
( A/WSN/1933 ) HA Protein Usage Guide
Storage:
|
Store it under sterile conditions at -70℃ . It is recommended that
the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw
cycles.
|
Reconstitution:
|
A hardcopy of COA with reconstitution
instruction is sent along with the products. Please refer to it for detailed
information.
|
Related Influenza Virus Research Tools
·
Influenza Virus
Research Tools
·
Influenza
Hemagglutinin & Antibody
·
Influenza
Hemagglutinin Proteins
·
H1N1
Hemagglutinin & Antibody
·
H1N1 Hemagglutinin
Proteins
·
A/WSN/1933
(A/WSN/33)
H1N1 ( A/WSN/1933 ) HA Protein Description
Influenza (flu) is a respiratory
infection in mammals and birds. This virus is divided into three main types (A,
B and C). Influenza A is found in a wide variety of bird and mammal species and
is further divided into subtypes based on differences in the membrane proteins
hemagglutinin (HA) and neuraminidase (NA). Influenza A H1N1 virus is a subtype
of influenza A virus. Some strains of H1N1 are endemic in humans and cause a
small fraction of all influenza-like illness and a small fraction of all
seasonal influenza. H1N1 strains caused a few percent of all human flu
infections in 2004-2005. Other strains of H1N1 are endemic in pigs (swine
influenza) and in birds (avian influenza). H1N1 was the most common cause of
human influenza (flu) in 2009. In June 2009, the World Health Organization
declared the new strain of swine-origin H1N1 as a pandemic. This strain is
often called swine flu by the public media. This novel virus spread worldwide
and had caused about 17,000 deaths by the start of 2010.
Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein
from the influenza virus, and comprises over 80% of the envelope proteins
present in the virus particle. In natural infection, inactive HA is matured
into HA1 and HA2 outside the cell by one or more trypsin-like,
arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding
of HA to sialic acid-containing receptors on the surface of its target cell
brings about the attachment of the virus particle to the cell and forms a
endosome. Low pH in endosomes induce an irreversible conformational change in
HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus
penetrates the cell and pours its contents including the RNA genome into the
cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane
and the endosomal membrane. Hemagglutinin plays a major role in the
determination of host range restriction and virulence
1.
Günther-Ausborn, S. et al., 2000, J.
Virol. 74: 2714-2720.
2.
Hoffman, LR. et al., 1997, J. Virol.
71: 8808-8820.
3.
Shinya K, et al., 2006,
Nature. 440 (7083): 435-6.
4.
Taubenberger JK, et al.,
2008. Annu Rev Pathol. 3: 499-522.
5.
Christophe F, et al., 2009, Science.
324:1557-1561.
6.
Morbidity and Mortality Weekly Report. 2009-04-24 .
p. 400-402.
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