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目前分類:【Abbkine品牌代理】抗氧化分析 (2)

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GPx穀胱甘肽過氧化物酶檢測 |Glutathione Peroxidase (GPx/GSH-Px) Activity Assay Kit貨號 KTB1640

CheKine™ Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Glutathione peroxidase (GSH-Px),簡稱為GSH-Px或GPx

Determination of GSH-Px/GPx activity in serum, plasma, plant tissue/cell lysates and other biological fluids.

measuring absorbance at 340 nm

abbkine商品插圖-300x207.jpg

 

穀胱甘肽 (Glutathione) 研究的意義

穀胱甘肽是含有谷氨酸 (glutamate),半胱氨酸(cysteine)和甘氨酸(glycine)的三肽,其中含有巰基(sulfhydryl groups)。它廣泛存在於動植物中。它具有抗氧化劑(antioxidant)和綜合解毒(detoxification)作用。其相關的輔酶(coenzyme)是NADPH。人體新陳代謝產生的自由基(free radicals)過多會破壞生物膜(biofilm),侵入生命大分子,加速人體衰老(aging),並誘發腫瘤(tumors)或動脈粥樣硬化(atherosclerosis) 的產生。穀胱甘肽具有增強生物體的抗氧化功能,促進生長以及增強生物體對人體不利環境的影響的功能。它的主要生理作用是清除體內的自由基。它也是一種很好的解毒劑,可以減弱有毒物質的毒性並從體內清除有毒物質。穀胱甘肽還促進生長並提高人體免疫力。穀胱甘肽(Glutathione)不僅可以用作藥物,還可以用作功能性食品的基礎材料,並且廣泛用於功能性食品中,例如抗衰老(anti-aging),增強免疫力(enhancing immunity)和抗腫瘤作用(anti-tumor)。

GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body's ability to resist radiation.

產品介紹:

GSH-Px /GPx穀胱甘肽過氧化物酶, Glutathione peroxidase (GSH-Px),簡稱為GSH-PxGPx。是在穀胱甘肽氧化還原循環中催化還原型穀胱甘肽(reduced glutathione , GSH)氧化的主要酶之一。 GSH-Px /GPx【穀胱甘肽過氧化物酶, Glutathione Peroxidase (GSH-Px)】不僅能特異性催化還原型穀胱甘肽與ROS之間的反應,形成氧化型穀胱甘肽GSSG,從而保護生物膜免受ROS破壞並維持細胞的正常功能。它還可以保護肝臟,提高人體免疫力,對抗有害金屬離子(metal ions)對人體的有害影響,並增強人體抵抗輻射(radiation)的能力。GSH-Px 【穀胱甘肽過氧化物酶, Glutathione Peroxidase (GSH-Px /GPx)】催化H2O2氧化GSH還原型穀胱甘肽(reduced glutathione , GSH),生成GSSG (氧化型穀胱甘肽GSSG)。穀胱甘肽還原酶(glutathione reductase , GR)催化NADPH還原GSSG(氧化型穀胱甘肽GSSG),從而再生GSH還原型穀胱甘肽(reduced glutathione , GSH),而NADPH氧化產生NADP +。 NADPH在340 nm處有一個特徵吸收峰,而NADP +沒有。通過測量340 nm處吸光度的降低速率來確定NADPH脫氫速率,以計算GSH-Px 【穀胱甘肽過氧化物酶, Glutathione Peroxidase (GSH-Px /GPx)】活性。

GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.

 

Product name CheKine™ Micro Glutathione Peroxidase (GSH-Px /GPx) Activity Assay Kit
Applications notes GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.
Kit components • Assay Buffer
• Substrate
• GR
• H2O2
Features & Benefits • Determination of GSH-Px activity in serum, plasma, plant tissue/cell lysates and other biological fluids.
Usage notes • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
• Prepare mixed reagents for use, and place on ice.
• The measuring process should be operated quickly and only 1 ~ 2 blank well should be needed.
• The reaction temperature has influence on the result. Keep the temperature at 25°C (for general species) or 37°C (for mammals).
Storage instructions Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body's ability to resist radiation.

 

產品貨號 CheKine產品品項        CheKine產品原理          
KTB1600

英文名稱: CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit

還原型穀胱甘肽(GSH)檢測

還原型穀胱甘肽(GSH)比色分析試劑盒 (KTB1600)利用DTNB與還原型穀胱甘肽反應形成黃色產物。在412 nm處測得的光密度可以直接反映樣品中的穀胱甘肽濃度。
KTB1610

英文名稱: CheKine™ Glutathione Oxidized (GSSG) Colorimetric Assay Kit

氧化型穀胱甘肽(GSSG)檢測

穀胱甘肽氧化(GSSG)比色分析試劑盒:還原型穀胱甘肽可與DTNB反應並生成2-硝基-5-巰基苯甲酸,在412nm波長處具有最大的光吸收率。
KTB1620

英文名稱: CheKine™ Glutathione Reductases (GR) Colorimetric Assay Kit

GR穀胱甘肽還原酶檢測

穀胱甘肽還原酶(GR)比色分析試劑盒:GR可以催化NADPH還原為GSSG,從而再生GSH,NADPH脫氫生成NADP +;NADPH在340 nm有一個特徵吸收峰,而NADP +在該波長沒有吸收峰。通過測量340 nm處吸光度的降低率來計算GR活性,從而確定NADPH脫氫率。
KTB1630

英文名稱: CheKine™ Glutathione S-Transferase (GST) Colorimetric Assay Kit

GST穀胱甘肽S-轉移酶分析

穀胱甘肽S-轉移酶(GST)比色分析試劑盒:GST催化GSH與CDNB的結合。共軛伴隨著在340nm處吸光度的增加。增加速率與樣品中的GST活性成正比。
KTB1640

英文名稱: CheKine™ Glutathione Peroxidase (GSH-Px/GPx) Colorimetric Assay Kit

GSH-Px /GPx穀胱甘肽過氧化物酶檢測

 穀胱甘肽過氧化物酶(GSH-Px)比色測定試劑盒:GSH-Px催化H2O2氧化GSH生成GSSG;穀胱甘肽還原酶(GR)催化NADPH還原GSSG從而再生GSH,而NADPH氧化產生NADP +。 NADPH在340 nm處有一個特徵吸收峰,而NADP +沒有。通過測量340 nm處吸光度的降低速率來確定NADPH脫氫速率,以計算GSH-Px活性。
KTB1650

英文名稱: CheKine™ Thioredoxin Reductase (TrxR) Colorimetric Assay Kit

硫氧還蛋白過氧化物酶(TPX)檢測

硫氧還蛋白還原酶(TrxR)比色測定試劑盒:TrxR可以催化NADPH還原DTNB從而生成TNB和NADP +。 TNB在412nm處具有特徵吸收峰。 TrxR活性可以通過測量412 nm處TNB的增加速率來計算。
KTB1660

英文名稱: CheKine™ Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit

硫氧還蛋白過氧化物酶(TPX)檢測 

硫氧還蛋白過氧化物酶(TPX)比色測定試劑盒:TPX催化H2O2氧化二硫蘇糖醇(DTT)。 H2O2的吸收波長為240nm。 TPX活性可以通過測量240nm處吸光度的降低速率並減去過氧化氫酶(CAT)催化的H2O2來計算。因此,該試劑盒可以同時測量樣品的TPX和CAT活性。

 活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 Abbkine 細胞膜染色 活細胞Lysosome染色

Product Name Catalog No
CheKine™ Coenzyme Ⅱ NADP(H) Colorimetric Assay Kit KTB1010
CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit KTB1011
CheKine™ Coenzyme Ⅰ NAD(H) Colorimetric Assay Kit KTB1020
CheKine™ NAD+ kinase (NADK) Activity Colorimetric Assay Kit KTB1022
CheKine™ Superoxide Dismutases (SOD) Activity Colorimetric Assay Kit KTB1030
CheKine™ Catalase (CAT) Activity Colorimetric Assay Kit KTB1040
CheKine™ Lipid Peroxidation (MDA) Colorimetric Assay kit KTB1050
CheKine™ Xanthine Oxidase Activity Colorimetric Assay Kit KTB1070
CheKine™ Superoxide anion Scavenging Capacity Colorimetric Assay Kit KTB1080
CheKine™ Lactate Colorimetric Assay Kit KTB1100
CheKine™ Lactate Dehydrogenase (LDH) Colorimetric Assay Kit KTB1110
CheKine™ Glucose Colorimetric Assay Kit KTB1300
CheKine™ Glucose Oxidase Activity (GOD) Colorimetric Assay Kit KTB1310
CheKine™ Hydrogen Peroxide (H2O2) Colorimetric Assay Kit KTB1041
CheKine™ Nitric Oxide (NO) Colorimetric Assay Kit KTB1400
CheKine™ Total Antioxidant Capacity (TAC) Colorimetric Assay Kit KTB1500
CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit KTB1600
CheKine™ Glutathione Oxidized (GSSG) Colorimetric Assay Kit KTB1610
CheKine™ Glutathione Reductases (GR) Activity Colorimetric Assay Kit KTB1620
CheKine™ Glutathione S-Transferase (GST) Colorimetric Assay Kit KTB1630
CheKine™ Glutathione Peroxidase (GSH-Px) Activity Colorimetric Assay Kit KTB1640
CheKine™ Thioredoxin Reductase (TrxR) Colorimetric Assay Kit KTB1650
CheKine™ Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit KTB1660
CheKine™ NADH Oxidase (NOX) Colorimetric Assay Kit KTB1021
CheKine™ Pyruvate Kinase (PK) Colorimetric Assay Kit KTB1120
CheKine™ Protein Carbonyl Colorimetric Assay Kit KTB1200
CheKine™ Tissue and Blood Alkaline Phosphatase (AKP/ALP) Activity Colorimetric Assay Kit KTB1700
CheKine™ Na⁺/K⁺-ATPase Activity Colorimetric Assay Kit KTB1800
CheKine™ Ca2+/Mg2+-ATPase Activity Colorimetric Assay Kit KTB1810
CheKine™ Monoamine Oxidase (MAO) Activity Colorimetric Assay Kit KTB1900
CheKine™ Polyphenol Oxidase (PPO) Activity
Colorimetric Assay Kit
KTB1140
CheKine™ Serum Potassium (K+) Colorimetric Assay Kit KTB2100
CheKine™ Triglyceride (TG) Colorimetric Assay Kit KTB2200
CheKine™ Free Cholesterol (FC) Colorimetric Assay Kit KTB2210
CheKine™ Total Cholesterol (TC) Colorimetric Assay Kit KTB2220
CheKine™ Pyruvate Acid (PA) Colorimetric Assay Kit KTB1121
CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit KTB1410
CheKine™ Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit KTB1420
CheKine™ Uric Acid (UA) Colorimetric Assay Kit KTB1510
CheKine™ Phosphoenolpyruvate Carboxylase (PEPC) Activity Colorimetric Assay Kit KTB1122
CheKine™ Toal Glutathione (T-GSH) Colorimetric Assay Kit KTB1670
CheKine™ Cysteine (Cys) Colorimetric Assay Kit KTB1450
CheKine™ Phenylalanine Ammonia Lyase (PAL) Activity Colorimetric Assay Kit KTB1160
CheKine™ Proline (PRO) Colorimetric Assay Kit KTB1430
CheKine™ Glutamate (Glu) Colorimetric Assay Kit KTB1440
CheKine™ γ-Glutamyl Cysteine Ligase (GCL) Activity Colorimetric Assay Kit KTB1680
CheKine™ Gamma-Glutamyl Transpeptidase (GGT) Activity Colorimetric Assay Kit KTB1690
CheKine™ Peroxidase (POD) Activity Colorimetric Assay Kit KTB1150
CheKine™ Plant Soluble Sugar Colorimetric Assay Kit KTB1320
CheKine™ Plant Flavonoids Colorimetric Assay Kit KTB1530
CheKine™ Plant Oligomeric Proantho Cyanidins (OPC) Colorimetric Assay Kit KTB1520
CheKine™ Plant Total Phenols (TP) Colorimetric Assay Kit KTB1540
CheKine™ Superoxide Anion Colorimetric Assay Kit KTB1210
CheKine™ Serum Sodium Colorimetric Assay Kit KTB2130
CheKine™ Serum Zinc Colorimetric Assay Kit KTB2140
CheKine™ Mitochondrial complex I Activity Assay Kit (colorimetric) KTB1850
CheKine™ Mitochondrial complexⅡ Activity Assay Kit (colorimetric) KTB1860
CheKine™ Mitochondrial complex Ⅲ Activity Assay Kit (colorimetric) KTB1870
CheKine™ Mitochondrial complex Ⅳ Activity Assay Kit (colorimetric) KTB1880
CheKine™ Mitochondrial complex Ⅴ Activity Assay Kit (colorimetric) KTB1890
CheKine™ Citrate Synthase (CS) Activity Assay Kit( Colorimetric) KTB1023
CheKine™ Total Iron-Binding Capacity (TIBC) Colorimetric Assay Kit KTB2150
CheKine™ Pyruvate Dehydrogenase (PDH) Activity Assay Kit (Colorimetric) KTB1270
CheKine™ Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit KTB1910
CheKine™ Acetyl Coenzyme A (Acetyl-CoA) Colorimetric Assay Kit KTB1260
CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit KTB1220
CheKine™ Succinate Dehydrogenase(SDH) Activity Assay Kit (Colorimetric) KTB1230
CheKine™ α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit (Colorimetric) KTB1240
CheKine™ Mitochondrial Isocitrate Dehydrogenase(ICDHm)Activity Colorimetric Assay Kit KTB1250
CheKine™ Blood Glucose Assay Kit(Colorimetric) KTB1330
CheKine™ Glycogen Assay Kit(Colorimetric) KTB1340
CheKine™ Total Carbohydrate Assay Kit(Colorimetric) KTB1350
CheKine™ Reducing Sugar(RS) Assay Kit(Colorimetric) KTB1360
CheKine™ Ceruloplasmin Activity Assay Kit ( Colorimetric) KTB1550
CheKine™ Advanced Oxidation Protein Products (AOPP) Assay Kit(Colorimetric) KTB1060
CheKine™ Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit KTB1091
CheKine™ Micro Hexokinase (HK) Activity Colorimetric Assay Kit KTB1123
CheKine™ 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit KTB1124
CheKine™ Amino Acid (AA) Colorimetric Assay Kit KTB1460
CheKine™ Pyruvate Decarboxylase (PDC) Colorimetric Assay Kit KTB1125
CheKine™ Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Colorimetric Assay Kit KTB1480
CheKine™ Lysine (LYS) Colorimetric Assay Kit KTB1470
CheKine™ Free Fat Acid (FFA) Colorimetric Assay Kit KTB2230
CheKine™ α-Amylase Activity Colorimetric Assay Kit KTB1370
CheKine™ β-Amylase Activity Colorimetric Assay Kit KTB1380
CheKine™ Starch Branching Enzyme(SBE) Activity Colorimetric Assay Kit KTB1390
CheKine™ Acetylcholinesterase (AchE) Activity Colorimetric Assay Kit KTB1710
CheKine™ Oxygen Radical Antioxidant Capacity (ORAC) Fluorometric Assay Kit KTB1501
CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit KTB1502
CheKine™ Alcohol Acyltransferase(AAT)Activity Colorimetric Assay Kit KTB1560
CheKine™ Fatty Acid Synthetase(FAS) Activity Colorimetric Assay Kit KTB2240
CheKine™ Aconitase (ACO) Activity Colorimetric Assay Kit KTB1290
CheKine™ Mitochondrial Malate Dehydrogenase(mMDH) Activity Colorimetric Assay Kit KTB1280
CheKine™ Low Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit KTB2260
CheKine™ High Density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit KTB2250
CheKine™ Tannin Colorimetric Assay Kit KTB1541
CheKine™ Tannase(TAN) Activity Colorimetric Assay Kit KTB1542
CheKine™ Creatine Kinase (CK) Activity Colorimetric Assay Kit KTB1012
CheKine™ 6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit KTB1013
CheKine™ Anthocyanidin Reductase(ANR) Activity Colorimetric Assay Kit KTB3010

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實驗設備2玻片掃描

產品貨號 品項 反應 測量儀器
 貨號KTA1001 活細胞和死細胞雙染色試劑盒 100個反應

活細胞綠色螢光染劑Ex/Em = 488/530 nm;

死細胞紅色螢光染料(Ex/Em = 535/617)

貨號 KTA2010

 

TUNEL細胞凋亡檢測試劑盒

 

50個反應 螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道(Ex / Em = 490nm / 520 nm)

貨號 KTC011001

 

CCK-8細胞增殖和細胞毒性試劑盒 500個反應 吸收光450nm
貨號KTC4003 TraKine™粒線體染色 50個反應/250個反應 Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100 Pro活細胞微管蛋白染色 50個反應/250個反應 Ex/Em = 500/520 nm
貨號KTC4001 Abbkine 細胞膜染色  100個反應/500個反應 Ex/Em = 484/501 nm
貨號BMD00063 DAPI Staining Solution 10MG/100MG Ex/Em: 358/461 nm (with DNA)
貨號KTC4210 活細胞Lysosome染色 50個反應/250個反應 deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030 細胞衰老檢測試劑盒(β-半乳糖苷酶) 100個反應 光學顯微鏡
貨號KTA1020 CCK-8細胞增殖和細胞毒性試劑盒 1000個反應/10,000個反應 吸收光450nm
貨號KTA2030

細胞增殖EdU Image試劑盒

(BrdU替代法)

100個反應/500個反應 AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400 一氧化Dan(NO)檢測試劑盒 

96個反應/

192個反應

colorimetric (540 nm)

檢測範圍:1-100 µM

貨號KTB1070 CheKine™黃嘌呤氧化酶檢測

96個反應/

480個反應

OD 450 nm

線性範圍15.6 -500 mU/

貨號KTD3001  BCA蛋白定量試劑盒

2000個反應/

5000個反應

OD 562 nm

相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80

50 to 1000 µg/ml.

貨號KTB1110 乳酸脫氫酶(LDH)測定試劑盒

96個反應/

480個反應

λmax= 450 nm

1- 20 U/mL of LDH

貨號PRP100153 IGF-I重組蛋白 200UG/1MG

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)

> 95 % as determined by SDS-PAGE

貨號 KTA2020 細胞週期染色檢測 100個反應/500個反應 Ex/Em=535/615 nm.
貨號BMC21400 PurKine™內毒素去除預裝離心柱

1ml×5

3mlx5

More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200 CheKine™蛋白羰基比色測定試劑盒 

48個反應/

96個反應

吸收光370 nm
貨號KTB1080 超氧陰離子清除測定試劑盒

48個反應/

96個反應

Superoxide anion scavenging rate D% =(∆∆ODControl - ∆∆ODSample)÷ ∆∆ODControl ×100%
貨號KTB1090 清除氫氧自由基能力測定試劑

48個反應/

96個反應

吸收光 536nm Hydroxyl radical scavenging rate D% =(ASample - AControl)÷(ABlank - AControl)×100%
貨號KTB2220 TC總膽固醇檢測試劑盒

48個反應/

96個反應

500nm Standard: 5 μmol/mL
貨號KTB1510 CheKine™尿酸(UA)比色分析試劑盒

48個反應/

96個反應

 吸收光505 nm

Standard: 5 μmol/mL.

貨號KTB1300 Glucose葡萄糖測定試劑盒

96個反應/

192個反應

 吸光值630nm

4- 300 mg/dL)

貨號KTB1100 Lactate  乳酸檢測試劑盒 

96個反應/

480個反應

colorimetric (450 nm)

0.03 mM-2 mM

貨號KTB1700 組織和血液鹼性磷酸酶( Alkaline Phosphatase, AKP / ALP)比色測定

96個反應/

96 X5盤

吸收光510 nm
貨號KTB1500 總抗氧化劑能力TAC分析

96個反應/

480個反應

593 nm

偵測範圍: 0.15-3 mM

貨號KTA0002 Annexin V-AbFluor™488細胞凋亡檢測試劑盒 20個反應/50個反應/100個反應 Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KET7013 EliKine™ Rat IL-6 ELISA Kit試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL

貨號KET0003 雌二醇(Estradiol , E2)試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍40 pg/ml-1500 pg/ml

貨號KET6032 TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒

96個反應/

96 X5盤

450 nm

7.8 pg/ml-500 pg/ml

貨號KET6033 VEGF血管內皮生長因子ELISA試劑盒 

96個反應/

96 X5盤

450 nm

偵測範圍: 31.25 pg/mL-2000 pg/mL

貨號KET7005 白介素-1β(Interleukin-1β, IL-1β)ELISA試劑盒

96個反應/

96 X5盤

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

偵測範圍: 15.6 pg/ml-1000 pg/ml

貨號KTE70003 小鼠黃嘌呤脫氫酶/氧化酶(XDH)ELISA試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。

貨號 A02051HRP His-tag標籤多株抗體-HRP  50ul/200ul WB (1:5000).
貨號A02170MGB Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠 100ul/400ul/2ml GKPIPNPLLGLDST epitope
貨號KTA4001 JC-1線粒體膜電位測定試劑盒

100個反應/

500個反應

(聚結的JC-1,Ex / Em = 585/590 nm)發出綠色螢光(JC-1單體,Ex / Em = 510/527 nm)

JC-1 Stain

CCCP (10 mM)  Assay Buffer (5×)

貨號KTB1600 穀胱甘肽(Glutathione)檢測 96個反應

412 nm

2-200 µg/mL

貨號BMD00063 DAPI Staining Solution 1MG/10MG/100MG λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001 細胞核/細胞質分離試劑盒

50個反應/

200個反應

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG Calcein AM鈣黃綠素活細胞螢光染劑 50ug/1mg/10mg

λEX/λEm: 494/517 nm (pH 8) (calcein)

 

貨號 KTB1660 硫氧還蛋白過氧化物酶(TPX)檢測  96個反應 240nm
貨號KTB1020 NAD/NADH 分析試劑盒

96個反應/

480個反應

OD565 nm

96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。

貨號KTB1070 CheKine™黃嘌呤氧化酶檢測

96個反應/

480個反應

OD 450 nm

線性範圍15.6 -500 mU/mL

貨號KTB1040 Catalase過氧化氫酶活性檢測試劑盒

96個反應/

480個反應

OD 540 nm

2-75 µM

貨號KTB1060 過氧化物酶(Peroxidases)分析試劑盒 96個反應

OD 664 nm

線性範圍為15.6-1000 µU/mL

貨號KTB1050 脂質過氧化檢測試劑盒(MDA) 96個反應

OD 532 nm

1-100 µM/L

貨號KTB1030 超氧化物歧化酶(SOD)活性測定試劑盒  96個反應 200-3.13 U/ml
貨號KTA1030 LDH細胞毒性測定試劑盒

96個反應/

480個反應

 OD 565 nm

10,00-100,0000 cells per well

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CheKine™活性氧(ROS)檢測分析試劑盒(螢光法)CheKine™ Reactive Oxygen Species (ROS) Detection Assay Kit | 貨號KTB1910

CheKine™ Reactive Oxygen Species (ROS) Detection Assay Kit (Fluorometric) 10 mM  50個反應( 10mM, 50ul)

/100個反應 (10mM, 100ul)  (以6well, 1ml體積為一個反應)

abbkine商品插圖-300x207.jpg

活性氧物種(Reactive Oxygen Species, Ros ) Ros是氧正常代謝的天然副產物,在細胞信號傳導(cell signaling)和體內平衡(vivo balance)中起重要作用。 但是,在氧化應激相關狀態(oxidative stress correlation state)下,ROS水平可以大大提高。 ROS的積累將嚴重破壞細胞結構(cell structure)。 氧化應激(Oxidative stress)在心血管疾病(cardiovascular disease),糖尿病(diabetes),骨質疏鬆(osteoporosis),中風(stroke),炎性疾病(inflammatory disease)以及神經退行性疾病(neurodegenerative diseases)和癌症(cancer)等研究中起著重要作用。 ROS檢測將有助於確定氧化應激(oxidative stress)如何調節各種細胞內途徑(intracellular pathways)。Reactive Oxygen Species, Ros is natural by-products of oxygen normal metabolism, play an important role in cell signaling and in vivo balance. However, in the oxidative stress correlation state, the ROS level can be greatly increased. The accumulation of ROS will seriously damage the cell structure. Oxidative stress plays an important role in the research of cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory disease, and neurodegenerative diseases and cancer,etc. ROS detection will help determine how oxidative stress adjusts various intracellular pathways.

Preparation of ROS probe: DCFH-DA is diluted with serum-free medium and the final concentration is 10 μM. ( 10mM, 50ul/100ul)

 

產品介紹: CheKine™活性氧(CheKine™ Reactive Oxygen Species , ROS)檢測分析試劑盒提供了一種簡單,靈敏,快速的ROS檢測方法。 ROS檢測基於螢光探針DCFH-DA。 DCFH-DA是一種可滲透細胞的靈敏探針,用於檢測細胞內活性氧(reactive oxygen species , ROS)。 該DCFH-DA探針可在穿過活細胞膜時被細胞中的酯酶水解為DCFH。 DCFH沒有螢光並且不能透過細胞膜,然後可以被ROS氧化並產生螢光DCF。 然後可以通過流式細胞儀或螢光顯微鏡檢測螢光,並據此分析細胞中活性氧(CheKine™ Reactive Oxygen Species , ROS)的含量。

網站規格小圖_工作區域 1.jpg

 

Kit components • DCFH-DA (10 mM)
Features & Benefits • provides a simple, sensitive, rapid ROS detection method.
• based on the fluorescent probe DCFH-DA .
• suitable for flow cytometry or fluorescence microscopy.
• Provides detailed sample preparation and results calculation methods.
Usage notes • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• The working concentration of positive control H2O2 should be 100 μM (Recommend during 50-200 μM depend on the cell types ). The level of Reactive Oxygen Species (ROS) will increase significantly after stimulated 30 min-4 h. The level of Reactive Oxygen Species (ROS) maybe very different for different cells while stimulating with the positive control. The stimulating time and the concentration of positive control could be prolonged or increased, if there is no obvious rise after 30 min . Or shorten the time or reduce the concentration of positive control, while the fluorescent signal is too strong.
• During the experiment, the fluorescent probe DCFH-DA can be diluted in 1: 2000-1: 5000, and make the final concentration of DCFH-DA to 2-5 μM if the signal of negative control also too strong. The time of probe incubating can be adjusted in 15-60 minutes, and minimize the exposure time while reading the film, and the exposure time of the control group and test groups should to be consistent.
• Reactive Oxygen Species (ROS) positive control (H2O2) is only used as a sample as a positive control, and not all test groups need positive control.
• Please wash away the unbound prode after incubating the prode , otherwise it will cause a high background.
• Try to shorten the detection time after incubating the probe (except for stimulation time) to reduce various possible errors.
Storage instructions Please store the whole kit at -20°C upon receipt ,and protect from light. The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

LncRNA SNHG4 regulates miR-10a/PTEN to inhibit the proliferation of acute myeloid leukemia cells(KTP3006-1.472)作者:Yuan Z, Wang W. 雜誌名稱:Hematology IF:1.472

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

BRAND/AGENCY/台灣代理商Abbkine台灣代理商

 

Abbkine Scientific Co., Ltd由一群志同道合的科學家和行銷專家,在2012年成立於美國加利福尼亞州,隨著亞洲市場的迅猛增長,Abbkine將總部搬遷至中國。結合了Abbkine美國技術和中國製造兩方面的巨大優勢,Abbkine致力於提供創新的、高品質的、買得起的實驗試劑盒、蛋白質、抗體以及其它研究工具,以期成為生命科學研究發展,藥物研發等領域的關鍵推動者。

 網站規格小圖 品牌介紹_工作區域 1.jpg

發展歷程: Abbkine致力於提供創新的、高品質的、買得起的實驗試劑盒、蛋白質、抗體以及其它研究工具以期成為生命科學研究發展,藥物研發等領域的關鍵推動者。Abbkine以特色的蛋白質生產與檢測試劑起家,致力於創新和研發各類抗體、生化試劑、蛋白質和實驗試劑盒,以期望為全球科研工作者提供革新的解決方案。我們很自豪地為客戶提供如下特色產品線,並會一如既往地開發新型的、高品質的、買得起的生命科學研究和診斷試劑產品。

2017年檢測細胞因數和激素的ELISA試劑盒應運而生: 根據市場需求,開發EliKine™系列ELISA試劑盒,涵蓋各類細胞因數和激素熱門指標,如白介素(IL)、腫瘤壞死因數(TNF)、干擾素(IFN)等明星級偶聯標記試劑盒全球發售:2018年憑藉先進的蛋白質偶聯技術和嚴格的品質控制,我們提供LinKine™偶聯標記試劑盒,在抗體、蛋白質、多肽或者其它生物分子上可直接標記HRP,Biotin,FITC,Cy3,AbFluor™以滿足科研應用,藥物研發以及診斷試劑盒開發的需求。2019年生化、細胞類產品線逐步擴充搭建細胞產品研發平臺,細胞狀態檢測、細胞染色、細胞提取、生化試劑盒產品得到越來越多全球客戶的認可。

 

  1. 全套表位標籤抗體和內參抗體,以及相應的定制偶聯產品
  2. 小包裝的磷酸化和甲基化特異性的抗體產品
  3. 最熱門的二抗產品:用於IF的IFKine系列產品,以及IP-WB的IPKine系列產品
  4. 全套高品質,買得起的蛋白質純化工具(填料,預裝柱和試劑盒)
  5. 暢銷的重組蛋白,包含各類細胞因數和生長因數
  6. 更多細胞功能分析試劑盒,ELISA試劑盒,以及細胞示蹤試劑

此外,Abbkine還建立了技術服務平臺,包括免疫學相關實驗服務(WB/IHC/IF/IP)、蛋白偶聯服務、蛋白表達、生化/細胞學實驗代測、Elisa實驗代測、抗體製備等,憑藉專業技術、設備和服務態度,為客戶提供一站式實驗解決方案。Abbkine時刻把握客戶需求變化,並積極應對、及時調整,使得Abbkine成為許多國際領先的研究機構和臨床診斷公司值得信賴的可靠供應商。

 

產品貨號 品項 反應 測量儀器
 貨號KTA1001 活細胞和死細胞雙染色試劑盒 100個反應

活細胞綠色螢光染劑Ex/Em = 488/530 nm;

死細胞紅色螢光染料(Ex/Em = 535/617)

貨號 KTA2010

 

TUNEL細胞凋亡檢測試劑盒

 

50個反應 螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道(Ex / Em = 490nm / 520 nm)

貨號 KTC011001

 

CCK-8細胞增殖和細胞毒性試劑盒 500個反應 吸收光450nm
貨號KTC4003 TraKine™粒線體染色 50個反應/250個反應 Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100 Pro活細胞微管蛋白染色 50個反應/250個反應 Ex/Em = 500/520 nm
貨號KTC4001 Abbkine 細胞膜染色  100個反應/500個反應 Ex/Em = 484/501 nm
貨號BMD00063 DAPI Staining Solution 10MG/100MG Ex/Em: 358/461 nm (with DNA)
貨號KTC4210 活細胞Lysosome染色 50個反應/250個反應 deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030 細胞衰老檢測試劑盒(β-半乳糖苷酶) 100個反應 光學顯微鏡
貨號KTA1020 CCK-8細胞增殖和細胞毒性試劑盒 1000個反應/10,000個反應 吸收光450nm
貨號KTA2030

細胞增殖EdU Image試劑盒

(BrdU替代法)

100個反應/500個反應 AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400 一氧化Dan(NO)檢測試劑盒 

96個反應/

192個反應

colorimetric (540 nm)

檢測範圍:1-100 µM

貨號KTB1070 CheKine™黃嘌呤氧化酶檢測

96個反應/

480個反應

OD 450 nm

線性範圍15.6 -500 mU/

貨號KTD3001  BCA蛋白定量試劑盒

2000個反應/

5000個反應

OD 562 nm

相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80

50 to 1000 µg/ml.

貨號KTB1110 乳酸脫氫酶(LDH)測定試劑盒

96個反應/

480個反應

λmax= 450 nm

1- 20 U/mL of LDH

貨號PRP100153 IGF-I重組蛋白 200UG/1MG

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)

> 95 % as determined by SDS-PAGE

貨號 KTA2020 細胞週期染色檢測 100個反應/500個反應 Ex/Em=535/615 nm.
貨號BMC21400 PurKine™內毒素去除預裝離心柱

1ml×5

3mlx5

More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200 CheKine™蛋白羰基比色測定試劑盒 

48個反應/

96個反應

吸收光370 nm
貨號KTB1080 超氧陰離子清除測定試劑盒

48個反應/

96個反應

Superoxide anion scavenging rate D% =(∆∆ODControl - ∆∆ODSample)÷ ∆∆ODControl ×100%
貨號KTB1090 清除氫氧自由基能力測定試劑

48個反應/

96個反應

吸收光 536nm Hydroxyl radical scavenging rate D% =(ASample - AControl)÷(ABlank - AControl)×100%
貨號KTB2220 TC總膽固醇檢測試劑盒

48個反應/

96個反應

500nm Standard: 5 μmol/mL
貨號KTB1510 CheKine™尿酸(UA)比色分析試劑盒

48個反應/

96個反應

 吸收光505 nm

Standard: 5 μmol/mL.

貨號KTB1300 Glucose葡萄糖測定試劑盒

96個反應/

192個反應

 吸光值630nm

4- 300 mg/dL)

貨號KTB1100 Lactate  乳酸檢測試劑盒 

96個反應/

480個反應

colorimetric (450 nm)

0.03 mM-2 mM

貨號KTB1700 組織和血液鹼性磷酸酶( Alkaline Phosphatase, AKP / ALP)比色測定

96個反應/

96 X5盤

吸收光510 nm
貨號KTB1500 總抗氧化劑能力TAC分析

96個反應/

480個反應

593 nm

偵測範圍: 0.15-3 mM

貨號KTA0002 Annexin V-AbFluor™488細胞凋亡檢測試劑盒 20個反應/50個反應/100個反應 Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KET7013 EliKine™ Rat IL-6 ELISA Kit試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL

貨號KET0003 雌二醇(Estradiol , E2)試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍40 pg/ml-1500 pg/ml

貨號KET6032 TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒

96個反應/

96 X5盤

450 nm

7.8 pg/ml-500 pg/ml

貨號KET6033 VEGF血管內皮生長因子ELISA試劑盒 

96個反應/

96 X5盤

450 nm

偵測範圍: 31.25 pg/mL-2000 pg/mL

貨號KET7005 白介素-1β(Interleukin-1β, IL-1β)ELISA試劑盒

96個反應/

96 X5盤

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

偵測範圍: 15.6 pg/ml-1000 pg/ml

貨號KTE70003 小鼠黃嘌呤脫氫酶/氧化酶(XDH)ELISA試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。

貨號 A02051HRP His-tag標籤多株抗體-HRP  50ul/200ul WB (1:5000).
貨號A02170MGB Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠 100ul/400ul/2ml GKPIPNPLLGLDST epitope
貨號KTA4001 JC-1線粒體膜電位測定試劑盒

100個反應/

500個反應

(聚結的JC-1,Ex / Em = 585/590 nm)發出綠色螢光(JC-1單體,Ex / Em = 510/527 nm)

JC-1 Stain

CCCP (10 mM)  Assay Buffer (5×)

貨號KTB1600 穀胱甘肽(Glutathione)檢測 96個反應

412 nm

2-200 µg/mL

貨號BMD00063 DAPI Staining Solution 1MG/10MG/100MG λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001 細胞核/細胞質分離試劑盒

50個反應/

200個反應

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG Calcein AM鈣黃綠素活細胞螢光染劑 50ug/1mg/10mg

λEX/λEm: 494/517 nm (pH 8) (calcein)

 

貨號 KTB1660 硫氧還蛋白過氧化物酶(TPX)檢測  96個反應 240nm
貨號KTB1020 NAD/NADH 分析試劑盒

96個反應/

480個反應

OD565 nm

96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。

貨號KTB1070 CheKine™黃嘌呤氧化酶檢測

96個反應/

480個反應

OD 450 nm

線性範圍15.6 -500 mU/mL

貨號KTB1040 Catalase過氧化氫酶活性檢測試劑盒

96個反應/

480個反應

OD 540 nm

2-75 µM

貨號KTB1060 過氧化物酶(Peroxidases)分析試劑盒 96個反應

OD 664 nm

線性範圍為15.6-1000 µU/mL

貨號KTB1050 脂質過氧化檢測試劑盒(MDA) 96個反應

OD 532 nm

1-100 µM/L

貨號KTB1030 超氧化物歧化酶(SOD)活性測定試劑盒  96個反應 200-3.13 U/ml
貨號KTA1030 LDH細胞毒性測定試劑盒

96個反應/

480個反應

 OD 565 nm

10,00-100,0000 cells per well

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