太鼎生物科技/太鼎食安科技

HRP抗體標誌套組

Lightning-Link® Horseradish Peroxidase (HRP)

Lightning-Link® Horseradish Peroxidase

Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.

The Lightning-Link® range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link® conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.

Horseradish Peroxidase (HRP) Label

Horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues which can be conjugated to labeled biomolecules using Lightning-Link®. The enzyme label can be visualized by chromogenic reactions; for example diaminobenzidine (DAB) in the presence of hydrogen peroxide (H₂0₂) is converted in to a water insoluble brown pigment. Other substrates which can be used to measure horseradish peroxidase activity include ABTS, TMB and TMBUS.

Click here for other labels available in the Lightning-Link® range.

Manufacturing

The technology is fully scalable (μg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.

3 x 10ug HRP (up to 40ug Ab) (701-0030)

1 x 100ug HRP (up to 400ug Ab) (701-0010)

3 x 100ug HRP (each up to 400ug Ab) (701-0000)

1 x 1mg HRP (up to 4mg Ab) (701-0002)

5 x 1mg HRP (each up to 4mg Ab) (701-0003)

1 x 5mg HRP (up to 20mg Ab) (701-0004)

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References:

Lightning-Link Horseradish Peroxidase

• Purification and Characterization of Shiga Toxin 2f, an Immunologically Unrelated Subtype of Shiga Toxin 2 - PLoS One (2013)
  
• Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, down's syndrome, preterm delivery and preeclampsia - Reproductive Biology and Endocrinology (2012)
  
• Neutralization capacity of measles virus H protein specific IgG determines the balance between antibody-enhanced infectivity and protection in microglial cells - Virus Research (2012)
  
• Measuring the specific activity of the protein tyrosine phosphatase Lyp - Journal of Immunological Methods (2012)
  
• Circulating levels of citrullinated and MMP-degraded vimentin (VICM) in liver fibrosis related pathology- Am J Transl Res (2012)
  
• Constitutive Formation of Caveolae in a Bacterium - Cell (2012)
  
• Measurement of MMP-9 and -12 degraded elastin (ELM) provides unique
  information on lung tissue degradation - BMC Pulmonary Medicine (2012)
  
• The carboxy-terminus of TFPIα is required for its interaction with factor V and Va - Journal of Thrombosis and Haemostasis (2012)
  
• Development of monoclonal antibody-based immunoassays for detection of Helicobacter pylori neutrophil-activating protein - Journal of Immunological Methods (2012)
  
• Sensitive quantitative assays for tau and phospho-tau in transgenic mouse models - Neurobiology of Aging (2012)
  
• A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants - Journal of Biomedicine and Biotechnology (2012)
  
• Porcine Reproductive and Respiratory Syndrome Virus Activates the Transcription of Interferon alpha/beta (IFN-α/β) in Monocyte-derived Dendritic Cells (Mo-DC) - Veterinary Microbiology (2012)
  
• A Human PrM Antibody That Recognizes a Novel Cryptic Epitope on Dengue E Glycoprotein - PLoS One (2012)
  
• A Conserved Tandem Cyclophilin-Binding Site in Hepatitis C Virus Nonstructural Protein 5A Regulates Alisporivir Susceptibility - Journal of Virology (2012)
  
• Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain - FEBS Journal (2011)
  
• Dual Neofunctionalization of a Rapidly Evolving Aquaporin-1 Paralog Resulted in Constrained and Relaxed Traits Controlling Channel Function during Meiosis Resumption in Teleosts - Mol Biol Evol (2011)
  
• Comparison of the efficiency of antibody selection from semi-synthetic scFv and non-immune Fab phage display libraries against protein targets for rapid development of diagnostic immunoassays - Journal of Immunological Methods (2011)
  
• Protein biomarkers in exfoliated cells collected from the human rectal mucosa: implications for colorectal disease detection and monitoring - International Journal of Colorectal Disease (2011)
  
• Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis - Fibrogenesis & Tissue Repair (2011)
  
• Canine specific ELISA for coagulation factor VII - The Veterinary Journal (2011)
  
• Aberrant splicing of the hRasGRP4 transcript and decreased levels of this signaling protein in the peripheral blood mononuclear cells in a subset of patients with rheumatoid arthritis - PLoS One (2011)
  
• MMP Mediated Degradation of Type VI Collagen Is Highly Associated with Liver Fibrosis – Identification and Validation of a Novel Biochemical Marker Assay - PLoS One (2011)
  
• Ubiquitin Fold Modifier 1 (UFM1) and Its Target UFBP1 Protect Pancreatic Beta Cells from ER Stress-Induced Apoptosis - PLoS One (2011)
  
• A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M) - Biomarkers (2011)
  
• Immunological detection of the type V collagen propeptide fragment, PVCP-1230, in connective tissue remodeling associated with liver fibrosis - Biomarkers (2011)
  
• Methodologic issues in the measurement of interleukin-16 in clinical blood samples using immunoassays - Cytokine (2011)
  
• Histidine-rich Glycoprotein Binds Fibrin(ogen) with High Affinity and Competes with Thrombin for Binding to the γ′-Chain - JBC (2011)
  
• Monoclonal antibody to novel cell surface epitope on Hsc70 promotes morphogenesis of bile ducts in newborn rat liver - Cell Stress and Chaperones (2010)
  
• Immunogenicity of attenuated measles virus engineered to express Helicobacter pylori neutrophil-activating protein - Vaccine (2010)
  
• Peptide Route Functionalization of ZnSe Crystals Preserves Activity and Structure of Proteins while Adsorption - The Journal of Physical Chemistry C (2010)
  
• Monoclonal antibody to novel cell surface epitope on Hsc70 promotes morphogenesis of bile ducts in newborn rat liver - Cell Stress and Chaperones (2010)
  
• Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells - JCB (2010)
  
• Regeneration of Dental-Pulp-like Tissue by Chemotaxis-Induced Cell Homing - Tissue Eng Part A. (2010)
  
• The Phosphatase Interactor NIPP1 Regulates the Occupancy of the Histone Methyltransferase EZH2 at Polycomb Targets – Nucleic Acids Research (2010)
  
• Extinction-Reconsolidation Boundaries: Key to Persistent Attenuation of Fear Memories - Science (2009)
  
• Development of a highly sensitive ELISA for aldosterone in mouse urine: Validation in physiological and pathophysiological states of aldosterone excess and depletion - Steroids (2009)
  
• Contributions to Neutropenia from PFAAP5 (N4BP2L2), a Novel Protein Mediating Transcriptional Repressor Cooperation between Gfi1 and Neutrophil Elastase - Molecular and Cellular Biology (2009)

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