【Caspase-3 Substrate DEVD-AFC】Caspase 3細胞凋亡受質DEVD-AFC。貨號#1007-1000。BIOVISION。－太鼎生物科技/太鼎食安科技

Caspase-3
Substrate DEVD-AFC

Caspase-3 Substrate DEVD-AFC

Catalog#: 1007-1000 | Size: 1000 assays

Alternate Name/Synonyms: Ac-DEVD-AFC,
Caspase-3 Substrate Fluorogenic

Peptide sequence: Ac-Asp-Glu-Val-Asp-AFC

Appearance: Liquid

Formulation: In DMSO (1 mM)

CAS Number: N/A

Molecular Formula: C₃₀H₃₃F₃N₅O₁₃

Molecular Weight: 729

Purity: >98% by HPLC

Solubility: N/A

Storage Temp.: -20°C

Shipping Conditions: gel pack

Description: Ready-to-use fluorometric substrate
for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino
acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in
poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be
quantified by fluorescent detection of free AFC after cleaved from the peptide
substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a
multi-well fluorescence plate reader. Alternatively, a shift in fluorescence
from blue to green upon cleavage can be visualized using a hand-held long-UV
lamp.

Handling: Potect from light and moisture