【Ni-IDA高親和力純化管柱-低成本His-tag標誌蛋白質的純化】#G239, Ni-IDA純化管柱, 純化來自細菌、酵母、昆蟲和哺乳動物細胞, 表達之his tag重組蛋白。－太鼎生物科技/太鼎食安科技

Ni-IDA高親和力純化管柱-低成本His-tag標誌蛋白質之純化

【Ni-IDA高親和力純化管柱】

Ni-IDA高親和力純化產品(G239)是一種用於純化任何重組表達系統，如細菌、酵母、昆蟲和哺乳動物細胞等表達系統表達的6xHis標籤的重組蛋白的瓊脂糖純化介質，它是通過三個結合位點與一種三齒螯合劑（亞氨基二乙酸）共價偶聯，該螯合劑可螯合Ni2+。該純化介質可在天然、變性或雜交條件下純化his標誌重組蛋白。結合到純化介質上的蛋白，用低pH緩衝液或咪唑溶液、甚至組氨酸溶液都可洗脫下來。

Ni-IDA高親和力純化管柱系列, (另有即用型預裝柱，便於His（6）標誌融合蛋白的純化。

Product	Size	Cat. No.	Price
Ni-IDA Agarose 1ml Columns	8 x 1ml	G239	$7000
Ni-IDA Agarose 5ml Columns	5 x 5ml	G240	$19000
Ni-IDA Agarose Resins	5ml	G250	$2200
Ni-IDA Agarose Resins	25ml	G251	$7800
Ni-IDA Agarose Resins	100ml	G253	$21800

主要特性:

結合能力強：>10 mg 6xHis 標誌蛋白/ml；

純化程式簡單，且可在天然或變性條件下純化蛋白；

pH穩定在3-13之間（短期pH 2－14）；

可多次使用。

應用:

在天然條件下純化多His-tag標誌蛋白。

在變性條件下純化在E.coli中表達的His-tag標誌蛋白

其它His tag標誌蛋白質純化-相關產品

Product		Raised In	Size	Concentration	Cat. No.		Price
His Tag Antibody (Rabbit Polyclonal)		Rabbit	100ug		G162		$0.00
His Tag Antibody (Mouse Monoclonal)		Mouse	100ug	100ug/100ul	G020		$4950
Product	Description					Cat No.		Price
GST Lysates	293 cells transfected with GST minigene, 26 kDa. 100µg/100µl					L004		$2600
Myc Lysates	293 cells transfected with Myc-tagged minigene, 37 kDa. 100µg/100µl					L001		$2600
His Lysates	293 cells transfected with His-tagged minigene, 50 kDa. 100µg/100µl					L003		$2600
HA Lysates	293 cells transfected with HA-tagged minigene, 55 kDa. 100µg/100µl					L002		$2600
D-Tag Lysates	293 cells transfected with D-Tag-tagged minigene, 60 kDa. 100µg/100µl					L074		$2600
GFP Lysates	293 cells transfected with GFP minigene, 30 kDa. 100µg/100µl					L079		$2600
V5 Lysates	293 cells transfected with V5-tagged fusion constructs, 130 kDa. 100µg/100µl					L082		$2600
β-Gal Lysates	293 cells transfected with β-Gal-tagged fusion constructs, 130 kDa. 100µg/100µl					L081		$2600
VSVG Lysates	293 cells transfected with a VSVG construct, 50 kDa. 100µg/100µl					L080		$2600