太鼎生物科技/太鼎食安科技

DNA Damage Quantification Kit

Catalog#: K253-25 | Size: 25 assays

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許虹宜0920-312382

www.biopioneer.com.tw

www.biovision.com

Kit Summary:
• Detection method- Absorbance (450 and 650 nm)
• Sample type- Purified damaged DNA
• Species reactivity- Mammalian
• Applications- Kit provides all necessary reagents for convenient determination of DNA damage (abasic sites) in sample DNA

Features & Benefits:
• Kit provides all necessary reagents for 25 reactions

Kit components:
• ARP Solution
• TE Buffer
• Glycogen Solution
• 0 ARP-DNA Standard
• 40 ARP-DNA Standard
• DNA Binding Solution
• HRP-Streptavidin
• 10X Wash Buffer
• HRP Developer
• 96-well Microplate (8 x 12 strips)

Description:
Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed during the course of base excision and repair of oxidized, deaminated or alkylated bases. It has been estimated that about 2x105 base lesions are generated per cell per day. The level of AP sites in cells can be a good indicator of DNA lesion and repair against chemical damage and cell aging. The DNA Damage Quantification Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with an aldehyde group which is the open ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using avidin-biotin assay followed by a colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA sample in 96-well plate format.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.