太鼎生物科技/太鼎食安科技

HT 8-oxo-dG ELISA kit II

WWW.TREVIGEN.COM

-8 - 氧代-2' -脫氧鳥苷（8 - oxo- dG），經常使用的DNA氧化損傷生物標誌物，是從 DNA鹼基切除修復途徑，並隨後運到唾液，尿液和血漿。為了測量全部的（8 - oxo- dG），Trevigen開發了經過驗證的競爭 ELISA試劑盒，定量的DNA，血漿，尿液和唾液中的8 - 氧代- dG的。

8-Oxo-2'-deoxyguanosine (8-oxo-dG), a frequently used biomarker of oxidative DNA damage, is removed from DNA by the base excision repair pathway, and subsequently transported into saliva, urine and plasma. In order to measure the total pool of 8-oxo-dG, Trevigen has developed a validated competitive ELISA kit that quantifies 8-oxo-dG in DNA, plasma, urine and saliva.  Trevigen’s HT 8-oxo-dG ELISA kit II, employs a 96 strip wells pre coated with 8-oxo-dG, an anti-8-oxo-dG monoclonal mouse antibody, an HRP conjugated secondary antibody, and colorimetric detection substrate to construct a high throughput assay flexible for your experimental design. The 8-OHdG monoclonal antibody binds competitively to 8-oxo-dG immobilized on pre-coated wells and in solution. Antibody bound to 8-oxo-dG in the sample is washed away while antibody bound to 8-oxo-dG attached to the well is retained. Detection of the retained antibody is performed using an HRP conjugate and colorimetric substrate. Product formation is inversely proportional to the amount of 8-oxo-dG present in the sample.

Catalog #	Product Name	Size
4380-096-K	HT 8-oxo-dG ELISA kit II	96 tests

HT 8-oxo-dG ELISA kit II Catalog #: 4380-096-K

Catalog #	Product Name	Size
4380-096-K	HT 8-oxo-dG ELISA kit II	96 tests

HT 8-oxo-dG ELISA kit II Catalog #: 4380-096-K

FEATURES:

• Colormetric, non-radioactive format
  
• High throughput 96 strip wells
  
• Dynamic range from 3.13 nM to 200 nM (0.89 ng/ml to 56.7 ng/ml)
  
• Sensitivity at 2 nM (0.57 ng/ml) 8-OHdG

APPLICATIONS:

For the detection and quantitation of 8-hydroxy-2’-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.

REFERENCES:

1. Nunomura, A., Perry, G., Pappolla, M.A., Wade, R., Hirai, K., Chiba, S., and Smith, M.A. (1999) Journal of Neuroscience 19:1959-1964.
2. Sancar, A., Lindsey-Boltz, L. A., Unsal-Kacmaz, K., and Linn, S. (2004) Annu. Rev. Biochem. 73: 39-85.
3. Wood, M. L., Dizdaroglu, M., Gajewski, E. and Essigmann, J. M. (1990) Biochemistry, 29, 7024-7032.
4. Marnett, L.J. (2000) Carcinogenesis. 21 (3): 361-370.
5. Tsou, T., Chen, C., Liu, T., and Yang, J. (1996) Carcinogenesis 17, 103-108