乳酸偵測分析套組/Lactate Assay Kit/K607-100
Catalog#: K607-100  | Size: 100 assays






















背景資料



乳酸鹽濃度的異常升高與多種疾病如糖尿病、酸毒症等疾病有密切的關係。L(+)-乳酸鹽是人體中乳酸鹽代謝的主要中間產物,並且存在於血液中。D(-)-乳酸鹽也存在,但含量很低,只有L(+)-乳酸鹽 的1-5%。過高的乳酸鹽濃度提示糖尿病和乳酸性酸毒症等疾病的存在。本試劑盒檢測L(+)-乳酸鹽的濃度。近期也有研究顯示,利用核磁共振檢測大腦中乳酸鹽含量,可瞭解人類大腦的老化程度;並發現乳酸鹽含量越高,大腦越老化。



產品描述



Biovision的乳酸檢測分析試劑盒提供了一種簡單、快速方便的方法來檢測生物學標本(人血液、細胞、培養基、發酵培養基等)中 L(+)-Lactate 的含量的方案,並且無需對標本進行預處理。在該乳酸鹽檢測試劑盒分析中,乳酸鹽特異性的與酶混合物反應並形成產物,該產物與乳酸鹽探針互相作用並產生顏色(λ= 570 nm)或者螢光(Ex/Em = 535/587 nm)。試劑盒檢測範圍為含0·001 -10 mM 乳酸鹽樣本。



產品特點



1、步驟簡單,只需要大約40分鐘即可完成檢測;2、操作快速並且方便;3、試劑盒內包括所有精確檢測乳酸鹽含量的必需組分



保存建議



-20保存



其他



BioVision的公司總部在美麗的三藩市灣地區,它是全球生命科學公司的領導者。BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力。包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因數和酶等多種產品,以及其他創新的研究工具。其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖症、細胞增殖與毒性、信號轉導、幹細胞研究等領域。作為多家知名品牌的中國總代理以及中國區域代理,艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務品質。









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【細胞培養液的條件】



Lactate分析,細胞培養的條件,可直接測量含有FBS的培養液。


(全部實驗都含有FBS)


A549細胞株為例,每24well中的細胞數為5X10424well中加入500ul含有FBSDMEM培養液,培養隔夜後,fresh培養液並取細胞培養液直接測量Lactate含量。


PS. 1ul10ul DMEM測試,10ul會使呈色變色~


 


Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect 0.001 -10 mM of various Lactate samples.


Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of Lactate colorimetrically and fluorometrically


Kit components:


• Lactate Assay Buffer
• Lactate Probe (in DMSO)
• Lactate Enzyme Mix
• L(+)-Lactate Standard (100 nmol/µl)


Description:
Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples. The kit can detect 0.001 -10 mM of various Lactate samples.


Storage Conditions:
-20°C

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