TdT、Brdu方法,專業品質值得信賴—Tunel assays
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TACS•XL® In Situ Apoptosis Detection Kits
TACS•XL®原位凋亡檢測試劑盒,使用bromodeoxyuridine (BrdU),經TdT作用加入凋亡細胞DNA碎片的3’ 端。這與使用生物素或地高辛標記核苷酸的Tunel方法相比,效率更高。通過使用biotin conjugated anti-BrdU antibody,streptavidin-HRP,提高了細胞內染色的信噪比。試劑盒分為:TACS•XL® DAB,TACS•XL® Blue Label和TACS•XL® Basic三種規格。
試劑盒組成:
特點:
• 信號強、背景低
• 較生物素和地高辛標記檢測,假陽性率更低
• DAB 或 TACS Blue Label™ 可供選擇
• 獨有的Cytonin™試劑,提高染色的透化作用
• TACS-Nuclease™試劑,供陽性對照片製作
• 可與螢光檢測試劑配套使用
應用:
• 石蠟或冰凍切片原位凋亡檢測
• 有助於凋亡形態學鑒別
儲存: 不同組分-20°C 、4°C 分別儲存
參考文獻:
1. Murray, N., L.A. Davidson, R.S. Chapkin, W.C. Gustafson, D.G. Schattenberg, and A.P. Fields. 1999. Overexpression of protein kinase C II induces colonic hyperproliferation and increased sensitivity to colon carcinogenesis. J. Cell. Biol. 45:699-711.
2. Bank, N., M. Kiroycheva, P.C. Singhal, G.M. Anthony, G.J. Southan, and C. Szabo. 2000. Inhibition of nitric oxide synthase ameliorates cellular injury in sickle cell mouse kidneys. Kidney Int. 58(1):82-89.
3. Li, X. and Z. Darzynkiewicz. 1995. Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Prolif. 28:571-579.
4. Gavrieli. Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501.
相關產品:
貨號 | 產品說明 | 規格 |
4828-30-DK | TACS.XL DAB Kit | 30 Samples |
4828-30-BK | TACS.XL Blue Labeling Kit | 30 Samples |
4828-30-K | TACS.XL Kit | 30 Samples |
4828-30-R | TACS.XL Replenisher Kit | 30 Samples |
4828-30-AC | TACS.XL Antibody Module | 30 Samples |
4828-30-BC | TACS Blue Label Detection Module | 30 Samples |
4828-30-DC | TACS.XL DAB Detection Module | 30 Samples |
4828-30-N | TACS.XL Nuclease Module | 15 Samples |
4828-30-04 | TACS B-dNTP Mix | 30 µl |
4828-30-06 | anti-BrdU antibody | 30 µl |
4828-30-12 | Strep-Diluent | 7.5 ml |
4828-30-18 | Methyl Green | 50 ml |
獨有的陽離子優化系統、Cytonin™透化技術—Tunel assays更清晰
TACS™ 2 TdT In Situ Apoptosis Detection Kits
The TACS™ 2 TdT Kits採用Trevigen所獨有的陽離子優化系統增強了對特殊組織的標記能力。選用高度純化的TdT enzyme,配以生物素化核苷酸,可供DAB、 TACS Blue Label™顯色或熒光檢測。
試劑盒組成:
特點:
• TdT酶原位標記
• 陽離子優化系統,信號強,背景低
• DAB、TACS Blue Label™或熒光檢測可供選擇
• 獨有的Cytonin™試劑,提高染色的透化作用
• TACS-Nuclease™試劑,供陽性對照片製作
應用:
• 石蠟或冰凍切片原位凋亡檢測
• 光學顯微鏡
• 螢光顯微鏡
• 流式細胞檢測
儲存: 不同組分-20°C 、4°C 、室溫分別儲存
參考文獻:
1. Cooper, L.F., J.C. Tiffee, J.P. Griffin, H. Hamano, and Z. Guo. 2000. Estrogen-induced resistance to osteoblast apoptosis is associated with increased hsp27 expression. J. Cell Physiology 185:401-407.
2. Kasahara, Y., R. Tuder, L. Taraseviciene-Stewart, T. LeCras, S. Abman, P. Hirth, J. Waltenberger, and N. Voelkel. 2000. Inhibition of VEGF receptors causes lung cell apoptosis and emphysema. J. Clin. Inves. 106:1311-1319.
3. Gavrieli, Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501.
4. Lovelace, C.I.P., J. Zhang, P.G. Vanek, and G.B. Collier. 1996. Detecting apoptotic cells in situ. Biomedical Products 21:76-77.
相關產品:
貨號 | 產品說明 | 規格 |
4810-30-CI | Cations (1 of each) | 30 µl each |
4810-30-CK | TACS 2 TdT-Core Kit | 30 Samples |
4810-30-K | TACS 2 TdT-DAB Kit | 30 Samples |
4810-30-R | TdT 2 Replenisher Kit | 30 Samples |
4811-30-K | TACS 2 TdT-Blue Label Kit | 30 Samples |
4812-30-K | TACS 2 TdT-Fluorescein Kit | 30 Samples |
4810-30-02 | TACS 2 TdT Labeling Buffer | 100 ml |
4810-30-03 | TACS 2 TdT Stop Buffer | 100 ml |
4810-30-04 | TACS 2 TdT dNTP | 30 µl |
4810-30-05 | TdT Enzyme | 30 µl |
4810-30-09 | Colbalt Cation (Sold as 4810-90-09) | 30 µl |
4810-30-10 | Magnesium Cation | 30 µl |
4810-30-14 | Manganese Cation (Sold as 4810-90-14) | 30 µl |
4810-90-09 | Cobalt Cation | 3 x 30 µl |
4810-90-10 | Magnesium Cation | 3 x 30 µl |
4810-90-14 | Manganese Cation | 3 x 30 µl |
4876-05-01 | Cytonin | 5 ml |
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