TdTBrdu方法,專業品質值得信賴­—Tunel assays



業務專員 許 虹宜 0920312382



TACS•XL® In Situ Apoptosis Detection Kits


      TACS•XL®原位凋亡檢測試劑盒,使用bromodeoxyuridine (BrdU),經TdT作用加入凋亡細胞DNA碎片的3’ 端。這與使用生物素或地高辛標記核苷酸的Tunel方法相比,效率更高。通過使用biotin conjugated anti-BrdU antibodystreptavidin-HRP,提高了細胞內染色的信噪比。試劑盒分為:TACS•XL® DABTACS•XL® Blue LabelTACS•XL® Basic三種規格。


 


試劑盒組成:


 



特點:


信號強、背景低


較生物素和地高辛標記檢測,假陽性率更低


• DAB TACS Blue Label™ 可供選擇


獨有的Cytonin™試劑,提高染色的透化作用


• TACS-Nuclease™試劑,供陽性對照片製作


可與螢光檢測試劑配套使用


應用:


石蠟或冰凍切片原位凋亡檢測


有助於凋亡形態學鑒別


儲存: 不同組分-20°C4°C 分別儲存


參考文獻:


1. Murray, N., L.A. Davidson, R.S. Chapkin, W.C. Gustafson, D.G. Schattenberg, and A.P. Fields. 1999. Overexpression of protein kinase C II induces colonic hyperproliferation and increased sensitivity to colon carcinogenesis. J. Cell. Biol. 45:699-711.


2. Bank, N., M. Kiroycheva, P.C. Singhal, G.M. Anthony, G.J. Southan, and C. Szabo. 2000. Inhibition of nitric oxide synthase ameliorates cellular injury in sickle cell mouse kidneys. Kidney Int. 58(1):82-89.


3. Li, X. and Z. Darzynkiewicz. 1995. Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Prolif. 28:571-579.


4. Gavrieli. Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501.


 


相關產品:
























































貨號



 產品說明



規格



 4828-30-DK



 TACS.XL DAB Kit



 30 Samples



 4828-30-BK



 TACS.XL Blue Labeling Kit



 30 Samples



 4828-30-K



 TACS.XL Kit



 30 Samples



 4828-30-R



 TACS.XL Replenisher Kit



 30 Samples



 4828-30-AC



 TACS.XL Antibody Module



 30 Samples



 4828-30-BC



 TACS Blue Label Detection Module



 30 Samples



 4828-30-DC



 TACS.XL DAB Detection Module



 30 Samples



 4828-30-N



 TACS.XL Nuclease Module



 15 Samples



 4828-30-04



 TACS B-dNTP Mix



 30 µl 



 4828-30-06



 anti-BrdU antibody



 30 µl



 4828-30-12



 Strep-Diluent



 7.5 ml 



 4828-30-18



 Methyl Green



 50 ml



 


 


 


獨有的陽離子優化系統、Cytonin™透化技術—Tunel assays更清晰


 


TACS™ 2 TdT In Situ Apoptosis Detection Kits


       The TACS™ 2 TdT Kits採用Trevigen所獨有的陽離子優化系統增強了對特殊組織的標記能力。選用高度純化的TdT enzyme,配以生物素化核苷酸,可供DAB TACS Blue Label™顯色或熒光檢測。


 


試劑盒組成:


 


 



特點:


• TdT酶原位標記


陽離子優化系統,信號強,背景低


• DABTACS Blue Label™或熒光檢測可供選擇


獨有的Cytonin™試劑,提高染色的透化作用


• TACS-Nuclease™試劑,供陽性對照片製作


 


應用:


石蠟或冰凍切片原位凋亡檢測


光學顯微鏡


螢光顯微鏡


流式細胞檢測


 


儲存: 不同組分-20°C4°C 、室溫分別儲存


 


參考文獻:


1. Cooper, L.F., J.C. Tiffee, J.P. Griffin, H. Hamano, and Z. Guo. 2000. Estrogen-induced resistance to osteoblast apoptosis is associated with increased hsp27 expression. J. Cell Physiology 185:401-407.


2. Kasahara, Y., R. Tuder, L. Taraseviciene-Stewart, T. LeCras, S. Abman, P. Hirth, J. Waltenberger, and N. Voelkel. 2000. Inhibition of VEGF receptors causes lung cell apoptosis and emphysema. J. Clin. Inves. 106:1311-1319.


3. Gavrieli, Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501.


4. Lovelace, C.I.P., J. Zhang, P.G. Vanek, and G.B. Collier. 1996. Detecting apoptotic cells in situ. Biomedical Products 21:76-77.


 


相關產品:












































































 貨號



 產品說明 



規格 



 4810-30-CI



 Cations (1 of each)



 30 µl each 



 4810-30-CK



 TACS 2 TdT-Core Kit



 30 Samples



 4810-30-K



 TACS 2 TdT-DAB Kit



 30 Samples



 4810-30-R



 TdT 2 Replenisher Kit



 30 Samples



 4811-30-K



 TACS 2 TdT-Blue Label Kit



 30 Samples



 4812-30-K



 TACS 2 TdT-Fluorescein Kit



 30 Samples



 4810-30-02



 TACS 2 TdT Labeling Buffer



 100 ml



 4810-30-03



 TACS 2 TdT Stop Buffer



 100 ml



 4810-30-04



 TACS 2 TdT dNTP



 30 µl



 4810-30-05



 TdT Enzyme



 30 µl



 4810-30-09



 Colbalt Cation (Sold as 4810-90-09)



 30 µl



 4810-30-10



 Magnesium Cation



 30 µl



 4810-30-14



 Manganese Cation (Sold as 4810-90-14)



 30 µl



 4810-90-09



 Cobalt Cation



 3 x  30 µl



 4810-90-10



 Magnesium Cation



 3 x  30 µl



 4810-90-14



 Manganese Cation



 3 x  30 µl



  4876-05-01



 Cytonin



 5 ml



< body>

arrow
arrow
    全站熱搜

    太鼎生物科技 發表在 痞客邦 留言(0) 人氣()