Fluoromount-G螢光封片劑 | 貨號: 0100-01 規格25ml

它適用於使用冷凍切片(frozen sections)和石蠟切片(paraffin sections)、whole mounts和細胞的顯微鏡應用。

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Fluoromount-G® (Clear liquid mounting medium) Fluoromount-G抗螢光淬滅封片劑是一種不含有螢光探針的水溶性封片劑，內含螢光保護劑，可大幅減緩螢光分子氧化褪色的情形。用來對最後一步染色是在水溶液體系中完成的玻片樣本進行封片，可以顯著減緩各種常見螢光染料的螢光。SouthernBiotech(SBA) Fluoromount-G系列螢光封片劑、抗淬滅封片劑，操作簡便用於長時間保存玻片，有一定抗淬滅作用，適用組織切片、細胞塗片/爬片等實驗。提供半永久性密封。據報導，Fluoromount-G® 的折射率為約為 1.4 70。Fluoromount-G®提供二種型式之螢光封片劑 (貨號#0100-01)、與含有DAPI染劑之DAPI螢光封片劑 (貨號#0100-20)。

Fluoromount-G使用方法 組織切片: 免疫染色、脫水、透明 (根據既定程序準備樣品並進行染色) ; 在水相最後一步之後，去除樣品周圍多餘的液體。並在 22 x 22 的面積上加入1~2滴(~50 μL) Fluoromount-G®封片劑覆蓋組織，蓋上蓋玻片。用紗布片輕輕按壓，使得多餘的封片劑將蓋玻片周圍封住；玻片置於空氣中乾燥5min。(提供半永久性密封)

產品狀態貯存: 水溶性透明液體，含0.1NaN3室溫避光貯存 。

產品型號 產品名稱 包裝
0100-01 Fluoromount-G螢光封片劑 25 mL
0100-20 Fluoromount-G螢光封片劑 (DAPI) 20 mL

封片最佳化: 建議封片後，以指甲油固定蓋玻片以避免樣品風乾，塗指甲油前需先將溢出的封片膠擦掉，而塗抹時蓋玻片邊緣與載玻片都要確實塗到，確實達到固定與防漏的效果。用顯微鏡觀察前也務必確認指甲油已乾，以避免指甲油沾染到物鏡。

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4 Fluoromount-G螢光封片劑貨號0100-01 規格25ml.jpeg

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Applications

Immunohistochemistry-Frozen Sections 1-28
Immunohistochemistry-Paraffin Sections 7,29,30
Immunohistochemistry-Whole Mount 31-33,48
Immunocytochemistry 12,17,30,34-64
In situ Hybridization 6,65
TUNEL 6,54 
Proximity Ligation Assay 66

引用文獻1-300x95.jpg

  1. Casas C, Isus L, Herrando-Grabulosa M, Mancuso FM, Borrás E, Sabidó E, et al. Network-based proteomic approaches reveal the neurodegenerative, neuroprotective and pain-related mechanisms involved after retrograde axonal damage. Sci Rep. 2015;5:9185. (IHC-FS, CY2, CY3, DAPI, NeuroTrace® 435/455 Blue)
  2. Cheng C, Wang W, Huang C, Du P, Yang J, Tsaur M. Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons. J Comp Neurol. 2016;524:846-73. (IHC-FS, AF488, AF594, AF647, TSA™ CY3)
  3. Cursio R, Miele C, Filippa N, Colosetti P, Auberger P, Van Obberghen E, et al. Tyrosine phosphorylation of insulin receptor substrates during ischemia/reperfusion-induced apoptosis in rat liver. Langenbecks Arch Surg. 2009;394:123-31. (IHC-FS, DAPI, Evans Blue, FLIVO®)
  4. del Mar N, von Buttlar X, Yu AS, Guley NH, Reiner A, Honig MG. A novel closed-body model of spinal cord injury caused by high-pressure air blasts produces extensive axonal injury and motor impairments. Exp Neurol. 2015;271:53-71. (IHC-FS, AF488, AF594, AF647, EYFP)
  5. Elsner RA, Hastey CJ, Olsen KJ, Baumgarth N. Suppression of long-lived humoral immunity following Borrelia burgdorferi infection. PLoS Pathog. 2015;11(17):e1004976. (IHC-FS, AF594, eFluor® 450, FITC)
  6. Gaillard D, Xu M, Liu F, Millar SE, Barlow LA. β-catenin signaling biases multipotent lingual epithelial progenitors to differentiate and acquire specific taste cell fates. PLoS Genet. 2015;11(5):e1005208. (IHC-FS, ISH, TUNEL, AF488, AF546, BCIP/NBT, DRAQ5®, SYTOX™ Green, TMR Red, X-gal, YFP)
  7. Herbig ME, Houdek P, Gorissen S, Zorn-Kruppa M, Wladykowski E, Volksdorf T, et al. A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin. Eur J Pharm Biopharm. 2015;95:99-109. (IHC-FS, IHC-PS, AF488, DAPI, MTT, Texas Red®)
  8. Hilton BJ, Assinck P, Duncan GJ, Lu D, Lo S, Tetzlaff W. Dorsolateral funiculus lesioning of the mouse cervical spinal cord at C4 but not at C6 results in sustained forelimb motor deficits. J Neurotrauma. 2013;30:1070-83. (IHC-FS, CY3, DyLight® 405)
  9. Imai DM, Feng S, Hodzic E, Barthold SW. Dynamics of connective-tissue localization during chronic Borrelia burgdorferi infection. Lab Invest. 2013;93:900-10. (IHC-FS, DAPI, NorthernLights™ 493)
  10. Kim YU, Lim H, Jung HE, Wetsel RA, Chung Y. Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS One. 2015;10(3):e0120294. (IHC-FS, DyLight® 594, DyLight® 650, Image-iT® FX Signal Enhancer , Pacific Blue™)
  11. Lang J, Ota T, Kelly M, Strauch P, Freed BM, Torres RM, et al. Receptor editing and genetic variability in human autoreactive B cells. J Exp Med. 2016;213:93-108. (IHC-FS, APC, DAPI, eFluor® 615)
  12. Laver CR, Metcalfe AL, Szczygiel L, Yanai A, Sarunic MV, Gregory-Evans K. Bimodal in vivo imaging provides early assessment of stem-cell-based photoreceptor engraftment. Eye. 2015;29:681-90. (IHC-FS, ICC, NorthernLights™ 557, Qtracker® 655)
  13. Lee CS, Hanna AD, Wang H, Dagnino-Acosta A, Joshi AD, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659. (IHC-FS, AF488, AF594, DAPI, Heochst 33342, PROTEOSTAT®)
  14. Lee J, Kapur M, Li M, Choi M, Choi S, Kim H, et al. MFN1 deacetylation activates adaptive mitochondrial fusion and protects metabolically challenged mitochondria. J Cell Sci. 2014;127:4954-63. (IHC-FS, DAB, NBT)
  15. Marsh SE, Abud EM, Lakatos A, Karimzadeh A, Yeung ST, Davtyan H, et al. The adaptive immune system restrains Alzheimer’s disease pathogenesis by modulating microglial function. Proc Natl Acad Sci USA. 2016;113:E1316-25. (IHC-FS, AF488, AF555, Amylo-Glo®)
  16. McBride EG, Rubel EW, Wang Y. Afferent regulation of chicken auditory brainstem neurons: rapid changes in phosphorylation of elongation factor 2. J Comp Neurol. 2013;521:1165-83. (IHC-FS, AF488, AF568, NeuroTrace® 640/660 Deep-Red)
  17. McClure S, Dudler L, Thorpe D, Hein WR. Analysis of cell division among subpopulations of lymphoid cells in sheep. I. Thymocytes. Immunology. 1988;65:393-9. (IHC-FS, ICC, DAB, Fast Red, Fast Blue, Hematoxylin)
  18. Tran HP, Shin E, Saito K, Nguyen XT, Chung YH, Jeong JH, et al. Protective potential of IL-6 against trimethyltin-induced neurotoxicity in vivo. Free Radic Biol Med. 2012;52:1159-74. (IHC-FS, AF488, AF594, Fluoro-Jade®)
  19. Michard E, Lima PT, Borges F, Silva AC, Portes MT, Carvalho JE, et al. Glutamate receptor-like genes form Ca2+ channels in pollen tubes and are regulated by pistil D-serine. Science. 2011;332:434-7. (IHC-FS, DRAQ5™, Marina Blue®)
  20. Murphy S, Henry M, Meleady P, Zweyer M, Mundegar RR, Swandulla D, et al. Simultaneous pathoproteomic evaluation of the dystrophin-glycoprotein complex and secondary changes in the mdx-4cv mouse model of Duchenne muscular dystrophy. Biology. 2015;4:397-423. (IHC-FS, AF488, Hoechst 33342, Rhodamine Red™-X, Sudan Black B)
  21. Piekarski N, Gross JB, Hanken J. Evolutionary innovation and conservation in the embryonic derivation of the vertebrate skull. Nat Commun. 2014;5:5661. (IHC-FS, AF488, Alizarin Red S, DAPI)
  22. Qvartskhava N, Lang PA, Görg B, Pozdeev VI, Ortiz MP, Lang KS, et al. Hyperammonemia in gene-targeted mice lacking functional hepatic glutamine synthetase. Proc Natl Acad Sci USA. 2015;112:5521-6. (IHC-FS, CY3, Eosin, FITC, Hematoxylin, Hoechst 34580)
  23. Sic H, Kraus H, Madl J, Flittner K, von Münchow AL, Pieper K, et al. Sphingosine-1-phosphate receptors control B-cell migration through signaling components associated with primary immunodeficiencies, chronic lymphocytic leukemia, and multiple sclerosis. J Allergy Clin Immunol. 2014;134:420-8. (IHC-FS, AF405, AF568, AF594, AmCyan, FITC)
  24. Stone LM, Tan S, Tam PP, Finger TE. Analysis of cell lineage relationships in taste buds. J Neurosci. 2002;22:4522-9. (IHC-FS, CY5, DAPI, FITC, Lissamine™ Rhodamine B, PI)
  25. Szabadfi K, Estrada C, Fernandez-Villalba E, Tarragon E, Setalo G Jr, Izura V, et al. Retinal aging in the diurnal Chilean rodent (Octodon degus): histological, ultrastructural and neurochemical alterations of the vertical information processing pathway. Front Cell Neurosci. 2015;9:126. (IHC-FS, AF488, AF568, CY3.5, DAPI, FITC)
  26. Tan X, Xue Y, Ma T, Wang X, Li JJ, Lan L, et al. Partial eNOS deficiency causes spontaneous thrombotic cerebral infarction, amyloid angiopathy and cognitive impairment. Mol Neurodegener. 2015;10:24. (IHC-FS, AF488, AF555, AF594, DAPI, DyLight® 488, Fluoro-Emerald, Thioflavin S)
  27. Wang H, Liu N, Zhang YP, Deng L, Lu Q, Shields CB, et al. Treadmill training induced lumbar motoneuron dendritic plasticity and behavior recovery in adult rats after a thoracic contusive spinal cord injury. Exp Neurol. 2015;271:368-78. (IHC-FS, Fast Blue, FITC, Fluoro-Gold, TRITC)
  28. Watanabe K, Nishimura Y, Oka T, Nomoto T, Kon T, Shintou T, et al. In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives. BMC Neurosci. 2010;11:116. (IHC-FS, AF488, Coumarin)
  29. Choi VW, Bigelow CE, McGee TL, Gujar AN, Li H, Hanks SM, et al. AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice. Mol Ther Methods Clin Dev. 2015;2:15022. (IHC-PS, AEC, Hematoxylin)
  30. Han JJ, Mhatre AN, Wareing M, Pettis R, Gao W, Zufferey RN, et al. Transgene expression in the guinea pig cochlea mediated by a lentivirus-derived gene transfer vector. Hum Gene Ther. 1999;10:1867-73. (IHC-PS, ICC, AMCA, GFP, Texas Red®)
  31. Berry R, Rodeheffer MS. Characterization of the adipocyte cellular lineage in vivo. Nat Cell Biol. 2013;15:302-8. (IHC-WM, AF647, BV421, dTomato, EGFP, HCS LipidTOX™ Deep Red)
  32. Fry TL, Atwood T, Dunbar MR. Evaluation of rhodamine B as a biomarker for raccoons. Hum-Wildl Interact. 2010;4:275-82. (IHC-WM, Rhodamine B)
  33. Jeffery E, Church CD, Holtrup B, Colman L, Rodeheffer MS. Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity. Nat Cell Biol. 2015;17:376-85. (IHC-WM, AF647, HCS LipidTOX™ Green)
  34. McRae RL. Investigating metal homeostasis in mammalian cells using high resolution imaging techniques [thesis]. Atlanta (United States): Georgia Institute of Technology; 2010. (ER-Tracker™ Green, LysoTracker® Red)
  35. Apaolaza PS, Delgado D, del Pozo-Rodríguez A, Gascón AR, Solinís MÁ. A novel gene therapy vector based on hyaluronic acid and solid lipid nanoparticles for ocular diseases. Int J Pharm. 2014;465:413-26. (ICC, AF488, Nile Red)
  36. Aporta A, Arbues A, Aguilo JI, Monzon M, Badiola JJ, de Martino A, et al. Attenuated Mycobacterium tuberculosis SO2 vaccine candidate is unable to induce cell death. PloS One. 2012;7(9):e45213. (ICC, CellTracker™ Green, CellTracker™ Orange, Hoechst 33342)
  37. Brady OA, Zhou X, Hu F. Regulated intramembrane proteolysis of the frontotemporal lobar degeneration risk factor, TMEM106B, by signal peptide peptidase-like 2a (SPPL2a). J Biol Chem. 2014;289:19670-80. (ICC, CF®488A, CF®568, CF®660C)
  38. Callé A, Ugrinova I, Epstein AL, Bouvet P, Diaz J, Greco A. Nucleolin is required for an efficient herpes simplex virus type 1 infection. J Virol. 2008;82:4762-73. (ICC, AF633, FluoProbes® 488, FluoProbes® 547H)
  39. Chemin K, Bohineust A, Dogniaux S, Tourret M, Guégan S, Miro F, et al. Cytokine secretion by CD4+ T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not microtubule organizing center polarity. J Immunol. 2012;189:2159-68. (ICC, AF546, AF647, CellTracker™ Blue)
  40. Desouza CV, Hamel FG, Bidasee K, O'Connell K. Role of inflammation and insulin resistance in endothelial progenitor cell dysfunction. Diabetes. 2011;60:1286-94. (ICC, AF647, PE, PE/CY7)
  41. Froger N, Orellana JA, Cohen-Salmon M, Ezan P, Amigou E, Sáez JC, et al. Cannabinoids prevent the opposite regulation of astroglial connexin43 hemichannels and gap junction channels induced by pro-inflammatory treatments. J Neurochem. 2009;111:1383-97. (ICC, AF488, AF555, Ethidium Bromide)
  42. Hammond GR, Schiavo G, Irvine RF. Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P2. Biochem J. 2009;422:23-35. (ICC, DAPI, NBD C6-Ceramide)
  43. Hangen E, De Zio D, Bordi M, Zhu C, Dessen P, Caffin F, et al. A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2. Cell Death Differ. 2010;17:1155-66. (ICC, AF488, dsRed, TO-PRO®-3)
  44. Hasenberg A, Hasenberg M, Männ L, Neumann F, Borkenstein L, Stecher M, et al. Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes. Nat Methods. 2015;12:445-52. (ICC, APC, AF532, SYTOX™ Green)
  45. Höbaus J, Tennakoon S, Heffeter P, Groeschel C, Aggarwal A, Hummel DM, et al. Impact of CYP24A1 overexpression on growth of colorectal tumour xenografts in mice fed with vitamin D and soy. Int J Cancer. 2016;138:440-50. (ICC, DAPI, DyLight® 550, GFP, MitoTracker® Red)
  46. Holt BD, Dahl KN, Islam MF. Differential sub-cellular processing of single-wall carbon nanotubes via interfacial modifications. J Mater Chem B. 2015;3:6274-84. (ICC, DAPI, FM® 4-64, GFP, Hoechst 33342, Oregon Green® 488)
  47. Holt BD, Roginskaya V, Van Houten B, Islam MF, Dahl KN. Dispersed single wall carbon nanotubes do not impact mitochondria structure or function, but technical issues during analysis could yield incorrect results. J Mater Chem B. 2017;5:369-74. (ICC, MitoTracker® Orange)
  48. Hsu C, Poché RA, Saik JE, Ali S, Wang S, Yosef N, et al. Improved angiogenesis in response to localized delivery of macrophage-recruiting molecules. PLoS One. 2015;10(7):e0131643. (IHC-WM, ICC, AF546, AF647, DAPI, DsRed, EGFP, mCherry)
  49. Jördens MS, Keitel V, Karababa A, Zemtsova I, Bronger H, Häussinger D, et al. Multidrug resistance-associated protein 4 expression in ammonia-treated cultured rat astrocytes and cerebral cortex of cirrhotic patients with hepatic encephalopathy. Glia. 2015;63:2092-105. (ICC, Hoechst 34580)
  50. Jyoti K, Kaur K, Pandey RS, Jain UK, Chandra R, Madan J. Inhalable nanostructured lipid particles of 9-bromo-noscapine, a tubulin-binding cytotoxic agent: in vitro and in vivo studies. J Colloid Interface Sci. 2015;445:219-30. (ICC, DAPI, Rhodamine 6G)
  51. Larsen ZH, Chander P, Joyner JA, Floruta CM, Demeter TL, Weick JP. Effects of ethanol on cellular composition and network excitability of human pluripotent stem cell-derived neurons. Alcohol Clin Exp Res. 2016;40:2339-50. (ICC, DAPI, DyLight® 488, DyLight® 550)
  52. Lee H, Shin D, Song H, Yuk J, Lee Z, Lee S, et al. Nanoparticles up-regulate tumor necrosis factor-α and CXCL8 via reactive oxygen species and mitogen-activated protein kinase activation. Toxicol Appl Pharmacol. 2009;238:160-9. (ICC, CdSe-ZnS Quantum Dots, LysoTracker® Green)
  53. Lerner N, Avissar S, Beit-Yannai E. Extracellular vesicles mediate signaling between the aqueous humor producing and draining cells in the ocular system. PLoS One. 2017;12(2):e0171153. (ICC, AF488, DAPI, DiD)
  54. Ma Y, Weiss LM, Huang H. Inducible suicide vector systems for Trypanosoma cruzi. Microbes Infect. 2015;17:440-50. (ICC, TUNEL, DAPI, FITC, TACS® 2 TdT Blue)
  55. McCormick PJ, Segarra M, Gasperini P, Gulino AV, Tosato G. Impaired recruitment of Grk6 and β-Arrestin 2 causes delayed internalization and desensitization of a WHIM syndrome-associated CXCR4 mutant receptor. PLoS One. 2009;4(12):e8102. (ICC, DyLight® 550, DyLight® 633)
  56. Park S, Lee SJ, Chung H, Her S, Choi Y, Kim K, et al. Cellular uptake pathway and drug release characteristics of drug-encapsulated glycol chitosan nanoparticles in live cells. Microsc Res Tech. 2010;73:857-65. (ICC, CY5.5, Ce6, LysoTracker® Red)
  57. Purdy AK, Campbell KS. SHP-2 expression negatively regulates NK cell function. J Immunol. 2009;183:7234-43. (ICC, AF350, AF488, AF635)
  58. Puri N, Kruhlak MJ, Whiteheart SW, Roche PA. Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly. J Immunol. 2003;171:5345-52. (ICC, AF488, AF546, DiI)
  59. Puskás LG, Fehér LZ, Vizler C, Ayaydin F, Rásó E, Molnár E, et al. Polyunsaturated fatty acids synergize with lipid droplet binding thalidomide analogs to induce oxidative stress in cancer cells. Lipids Health Dis. 2010;9:56. (ICC, Oil Red O)
  60. Riebeling C, Allegood JC, Wang E, Merrill AH Jr, Futerman AH. Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J Biol Chem. 2003;278:43452-9. (ICC, FITC, MitoTracker® Deep Red, TRITC)
  61. Schaller T, Appel N, Koutsoudakis G, Kallis S, Lohmann V, Pietschmann T, et al. Analysis of hepatitis C virus superinfection exclusion by using novel fluorochrome gene-tagged viral genomes. J Virol. 2007;81:4591-603. (ICC, AF488, DAPI, GFP, RFP)
  62. Tekle YI, Anderson OR, Lecky AF. Evidence of parasexual activity in "asexual amoebae" Cochliopodium spp. (Amoebozoa): extensive cellular and nuclear fusion. Protist. 2014;165:676-87. (ICC, AF488, AF594, CellMask™ Orange)
  63. Thekkedath R, Koshkaryev A, Torchilin VP. Lysosome-targeted octadecyl-rhodamine B-liposomes enhance lysosomal accumulation of glucocerebrosidase in Gaucher's cells in vitro. Nanomedicine (Lond). 2013;8:1055-65. (ICC, DyLight® 350, LysoTracker® Red)
  64. Wolins NE, Rubin B, Brasaemle DL. TIP47 associates with lipid droplets. J Biol Chem. 2001;276:5101-8. (ICC, BODIPY® 493/503)
  65. Gauthier LR, Granotier C, Hoffschir F, Etienne O, Ayouaz A, Desmaze C, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012;69:629-40. (FISH, CY3, DAPI, FITC)
  66. Chabaud M, Heuzé ML, Bretou M, Vargas P, Maiuri P, Solanes P, et al. Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. Nat Commun. 2015;6:7526. (PLA, AF488)
  67. Kruspe M, Thieme H, Guntinas-Lichius O, Irintchev A. Motoneuron regeneration accuracy and recovery of gait after femoral nerve injuries in rats. Neuroscience. 2014;280:73-87. (IHC, Fluoro-Emerald, Fluoro-Ruby®)
  68. Lewis MD, Behrends J, Sá E Cunha C, Mendes AM, Lasitschka F, Sattler JM, et al. Chemical attenuation of Plasmodium in the liver modulates severe malaria disease progression. J Immunol. 2015;194:4860-70. (IHC, AF488, AF555, AF660, Hoechst 33258)
  69. Lomeli N, Di K, Czerniawski J, Guzowski JF, Bota DA. Cisplatin-induced mitochondrial dysfunction is associated with impaired cognitive function in rats. Free Radic Biol Med. 2017;102:274-86. (IHC, FD Rapid GolgiStain™)
  70. Phillips KG, Velasco CR, Li J, Kolatkar A, Luttgen M, Bethel K. et al. Optical quantification of cellular mass, volume, and density of circulating tumor cells identified in an ovarian cancer patient. Front Oncol. 2012;2:72. (Refractive Index)

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