Fluoromount-G螢光封片劑 | 貨號: 0100-01 規格25ml

它適用於使用冷凍切片(frozen sections)和石蠟切片(paraffin sections)、whole mounts和細胞的顯微鏡應用。

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Fluoromount-G® (Clear liquid mounting medium) Fluoromount-G抗螢光淬滅封片劑是一種不含有螢光探針的水溶性封片劑，內含螢光保護劑，可大幅減緩螢光分子氧化褪色的情形。用來對最後一步染色是在水溶液體系中完成的玻片樣本進行封片，可以顯著減緩各種常見螢光染料的螢光。SouthernBiotech(SBA) Fluoromount-G系列螢光封片劑、抗淬滅封片劑，操作簡便用於長時間保存玻片，有一定抗淬滅作用，適用組織切片、細胞塗片/爬片等實驗。提供半永久性密封。據報導，Fluoromount-G® 的折射率為約為 1.4 70。Fluoromount-G®提供二種型式之螢光封片劑 (貨號#0100-01)、與含有DAPI染劑之DAPI螢光封片劑 (貨號#0100-20)。

Fluoromount-G使用方法 組織切片: 免疫染色、脫水、透明 (根據既定程序準備樣品並進行染色) ; 在水相最後一步之後，去除樣品周圍多餘的液體。並在 22 x 22 的面積上加入1~2滴(~50 μL) Fluoromount-G®封片劑覆蓋組織，蓋上蓋玻片。用紗布片輕輕按壓，使得多餘的封片劑將蓋玻片周圍封住；玻片置於空氣中乾燥5min。(提供半永久性密封)

產品狀態貯存: 水溶性透明液體，含0.1NaN3室溫避光貯存 。

產品型號 產品名稱 包裝
0100-01 Fluoromount-G螢光封片劑 25 mL
0100-20 Fluoromount-G螢光封片劑 (DAPI) 20 mL

封片最佳化: 建議封片後，以指甲油固定蓋玻片以避免樣品風乾，塗指甲油前需先將溢出的封片膠擦掉，而塗抹時蓋玻片邊緣與載玻片都要確實塗到，確實達到固定與防漏的效果。用顯微鏡觀察前也務必確認指甲油已乾，以避免指甲油沾染到物鏡。

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4 Fluoromount-G螢光封片劑貨號0100-01 規格25ml.jpeg

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Applications

Immunohistochemistry-Frozen Sections 1-28
Immunohistochemistry-Paraffin Sections 7,29,30
Immunohistochemistry-Whole Mount 31-33,48
Immunocytochemistry 12,17,30,34-64
In situ Hybridization 6,65
TUNEL 6,54 
Proximity Ligation Assay 66

引用文獻1-300x95.jpg

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