亞硝酸鹽檢測試劑盒 (Griess Reagent) | 貨號k544-200 (abcam ab234044)
亞硝酸鹽(Nitrite; NO2 - )累積量測定套組-Griess Reagent
在幾乎每個生物體中都發現了Nitrogen-based ions氮基離子,Nitrogen-based ions亞硝酸鹽和nitrate硝酸鹽。此外,在哺乳動物中發現內源亞硝酸鹽水平,也可從dietary sources膳食來源獲得。在人類中,nitrit亞硝酸鹽 (nitrite) 進一步代謝為一氧化氮(Nitric Oxide)和其他活性氮物質(reactive nitrogen species, nitrogen oxides氮氧化物)。硝酸鹽 - 亞硝酸鹽 - NO生化途徑因其參與細胞信號傳導(cell signaling),缺氧依賴性反應(hypoxia-dependent response )和血流調節而眾所周知。最近的研究表明線粒體中亞硝酸鹽還原為氮氧(Nitrogen Oxygen )。具體而言,肌紅蛋白(myoglobin)和黃嘌呤氧化還原酶(xanthine oxidoreductase)可在低氧條件下產生NO,從而導致線粒體呼吸。BioVision的亞硝酸鹽檢測試劑盒使用Griess Reagent,這是一種用於估算亞硝酸鹽的常用測量方式。在該測定中,使用Griess試劑I將亞硝酸鹽還原為氮氧化物。然後,氮氧化物與Griess Reagent II反應形成穩定的產物,其可通過其在OD 540nm處的吸光度來檢測。一個步驟快速簡單分析,檢測低至1 nmol /孔的亞硝酸鹽水平。
ELISA儀器設備請按此 (標配405、450、492、630 nm濾片/最高可擴充至7濾片)(k544 λ= 540 nm)
Kit Summary | • Detection method- Absorbance (540 nm) • Species reactivity- Mammalian • Applications- BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process. |
Detection Method | Detection method- Absorbance (540 nm) |
Species Reactivity | Eukaryotes |
Applications | Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process. |
Features & Benefits | • Simple two-step procedure; takes only ~1-1.5 hours • Fast and convenient • BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of total nitrite. |
Kit Components | • Assay Buffer • Griess Reagent R1 • Griess Reagent R2 • Nitrite Standard |
留言列表