Tyrosinase酪氨酸酶活性測定試劑盒（呈色法） | 貨號K742-100 (Abcam ab25289 )

The first non-radioactive kit on the market that measure Tyrosinase activity in biological samples

食品化學/皮膚相關研究中酪氨酸酶活性的分析

 

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Tyrosinase酪氨酸酶（EC 1.14.18.1）是一種銅結合酶(copper-binding enzyme)，可在從細菌(bacteria)、真菌(fungi)、到哺乳動物(mammals)的各種物種中表達。它參與黑色素(melanin)合成途徑的兩個連續反應; 首先是單酚(monophenol)的羥基化(hydroxylation)，其次是鄰二酚(ortho-diphenol)轉化為醌(quinone)。然後醌(quinone)進行一系列反應，包括聚合形成黑色素(melanin)。Tyrosinase酪氨酸酶對農業產業非常感興趣，因為它會引起水果，蔬菜和蘑菇的褐變(browning)，以及化妝品工業，因為它會導致皮膚變黑(skin darkening)。因此，酪氨酸酶抑制劑(tyrosinase inhibitors) 的開發和篩選對於諸如色素沉著過度(hyperpigmentation)和黃褐斑(melasma)等病症非常有用。酪氨酸酶活性(Tyrosinase activity)在黑素瘤中顯著增加。因此，酪氨酸酶活性的檢測可能有希望作為黑素瘤的特異性診斷測試，並且可用於監測患者對黑素瘤(melanoma)治療的反應。 BioVision的酪氨酸酶活性檢測試劑盒(Tyrosinase Activity Assay Kit, K742)是一種簡單one-step，基於平板的檢測，用於測量生物樣品中的酪氨酸酶活性。在該測定中，酪氨酸酶催化酚類底物(phenolic substrate)轉化為醌中間體(Quinone intermediate)，其與酪氨酸增強劑反應形成高度穩定的(chromophore)，在510nm處具有吸光度。該測定可以檢測生物樣品中低至30μU的酪氨酸酶。

 

Cat # +Size K742-100
Size 100 assays
Kit Summary • Detection method- Absorbance (510 nm)
• Applications- Measurement of Tyrosinase Activity in various tissue/cell lysates
- Analysis of Tyrosinase activity in food chemistry/skin-related research
Detection Method Absorbance (510 nm)
Species Reactivity Eukaryotes
Applications • Measurement of Tyrosinase Activity in various tissue/cell lysates
• Analysis of Tyrosinase activity in food chemistry/skin-related research
Features & Benefits • First non-radioactive assay in the market
• Simple, rapid & convenient
• The assay can detect Tyrosinase Activity less than 30 µU in biological samples.
Kit Components • Tyrosinase Assay Buffer
• Tyrosinase Substrate
• Tyrosinase Enhancer
• Chromophore Standard
• Tyrosinase Positive Control

 

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