JC-1線粒體膜電位測定試劑盒 | 貨號CAT # KTA4001
Mitochondrial Membrane Potential Assay Kit (JC-1)
Fig. Hela cells stained with Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1). A: Red fluorescence indicates healthy mitochondria, B: Green fluorescence indicates mitochondria in poor health (30min incubation in 20uM CCCP).
Abbkine線粒體膜電位測定試劑盒（Mitochondrial Membrane Potential Assay Kit , JC-1貨號KTA4001）提供了一種簡單方法，基於碳菁染料(carbocyanine dye ) JC-1通過檢測線粒體跨膜電位的變化來區分健康細胞和凋亡細胞(apoptotic cells)。在健康細胞中，這種染料在粒腺體中積累(accumulates)和聚集(aggregates)，形成鮮紅色的螢光團塊。耗散線粒體膜電位都會阻止JC-1染料在線粒體中積聚，因此該染料以其單體形式保留在細胞質中，導致從紅色的轉變（聚結的JC-1，Ex / Em = 585/590 nm）發出綠色螢光（JC-1單體，Ex / Em = 510/527 nm）。 JC-1可用作多種細胞類型（包括肌細胞和神經元）以及完整組織和分離的粒腺體中粒腺體潛力的指標。該試劑盒包含CCCP，CCCP導致質子梯度解偶聯，該質子梯度在電子傳輸鏈中電子載體的正常活動過程中建立，並因此消除了線粒體的電化學勢，可以用作防止JC-1聚集的對照組。
| Product name | Mitochondrial Membrane Potential Assay Kit (JC-1) |
| Kit components | • JC-1 Stain • CCCP (10 mM) • Assay Buffer (5×) |
| Features & Benefits | • Measure mitochondrial membrane potential as an indicator of cell health. • Use JC-1, a fluorescent, lipophilic, cationic dye. • Red fluorescence indicates healthy mitochondria and green fluorescence indicates mitochondria in poor health. • Optimized for flow cytometery or fluorescent microscopy. • Contain CCC (Potent mitochondrial oxidative phosphorylation uncoupler). |
| Usage notes | • JC-1 Stain is difficult to prepare due to its low solubility. Make sure JC-1 Stain is completely thawed and warmed to room temperature before using. • JC-1 Stain is light senstive. Incubations need to be done in the dark. |
| Storage instructions | Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipmen |
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