Cy5抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#781-0010)

 Cyanine Dye 5 (Cy5) 抗體標誌directly labeling kit (貨號#781-0010)，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

cY5 螢光dye特性

Cyanine Dye 5 (Cy5) has a strong absorption peak at 643nm and a secondary absorption peak at 605nm. The maximum emission peak is at 667nm, with an excitation coefficient of 2.5 x10⁵ cm^-1M^-1. Cy5 can be used as an alternative to AlexaFluor 647, and the phycobiliprotein Allophycocyanin.

Lightning-link抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-link螢光dYE抗體標誌---期刊發表:

Lightning-Link Cy3 ---期刊發表

Cy3抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#780-0010)

Cyanine Dye 3 (Cy3) 抗體標誌directly labeling kit (貨號#780-0010)，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

Cy3 螢光dye特性

Cyanine Dye 3 (Cy3) has a strong absorption peak at 550nm and a secondary absorption peak at 520nm. The maximum emission peak is at 570nm, with an excitation coefficient of 1.5 x10⁵ cm^-1 M^-1. Cy3 can be used as an alternative to Alexa Fluor 555, DY-550, Rhodamine Red-X and TAMRA [5(6)-Carboxytetramethylrhodamine].

Lightning-link抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-link螢光蛋白抗體標誌---期刊發表:

Lightning-Link Cy3 ---期刊發表

Atto 565抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#738-0010)

Atto565抗體標誌directly labeling kit (貨號#738-0010)，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

AtTO 565 螢光dye特性

Atto565 is one of a new generation of fluorescent labels. It has a strong absorption at 563nm, high fluorescence at 592nm (extinction coefficient 1.2 x10⁵ cm^-1M^-1) and quantum yield.

Lightning-link抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-link螢光蛋白抗體標誌---期刊發表:

Lightning-Link Cy3 ---期刊發表

Atto 488抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#733-0010)

 Atto 488抗體標誌directly labeling kit (貨號#733-0010)，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

AtTO 488 螢光DYE特性

Atto488 is one of a new generation of fluorescent labels, which has been optimised for excitation with an argon laser. It has a strong absorption at 501nm, high fluorescence at 523nm (extinction coefficient 9.0 x10⁴ cm^-1M^-1) and high quantum yield

Lightning-link抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-link螢光蛋白抗體標誌---期刊發表:

Lightning-Link Cy3 ---期刊發表

PerCP抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#718-0010)

  Peridinin-Chlorophyll-Protein Complex (PerCP) 抗體標誌directly labeling kit (貨號#718-0010)，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

R-PE螢光蛋白特性

Peridinin-Chlorophyll-Protein Complex (PerCP)

PerCP is a small 35kDa phycobiliprotein isolated from red algae. It has a strong absorption peak at 482nm and a secondary absorption peak at 442nm. PerCP has a large stokes shift (195nm) with its maximum emission peak at 677nm. PerCP is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting.

Lightning-link抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-link螢光蛋白抗體標誌---期刊發表:

Lightning-Link R-PE ---期刊發表

APC抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#705-0010)

  Allophycocyanin (APC)抗體標誌directly labeling kit (貨號#705-0010)，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

APC螢光蛋白特性

Allophycocyanin is a large 105kDa fluorescent phycobiliprotein commonly used in FACS analysis. It absorbs at 650nm and emits at 662nm.

Lightning-link抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-link螢光蛋白抗體標誌---期刊發表:

 抗體純化套組 Abselect kit

移除溶液中BSA、Glycine、Tris、Azide等殘留分子，純化後溶液，可直接應用於抗體標誌套組 (lightning-link, innova biosciences)

BSA、Gelatin、Glycine移除--àAbSelect 抗體-----純化系統

適合應用: 市售抗體。預移除溶液中BSA、Glycine、Tris、Azide等殘留分子

純化體積100ul至500ul

AbSelect抗體純化套組 (860-0010), 特別針對市售抗體保存液內, 含有BSA, Glycine. Tris. Azide等amino group干擾物質殘留。純化後的抗體, 可直接應用於抗體標誌試劑 (lightning-link一個步驟完成抗體標誌試驗)。

不須經由dialysis等純化步驟。抗體的體積可經由100ul至0.5ml， 每次純化, 可以純化>500ug抗體量。

 Ps. 蛋白質濃縮及buffer exchange之動作, 無法有效移除抗體內的小分子, 須經由Abselect 抗體純化系統來執行。

AbSelect Antibody Purification System

Commercially available antibodies often contain
substances (e.g. BSA, glycine, tris, azide) that interfere in labeling
reactions. The AbSelect™ Purification Kit quickly removes these contaminants.
It can also be used to purify antibodies from crude samples such as ascites
fluid or immune serum.

The antibody
to be purified or cleaned up ideally is in a volume of 100ul to 0.5ml. Up to
500µg of antibody can be purified in each run. The AbSelect method involves
capture of the antibody on protein A resin and the removal of unwanted
substances by a simple wash procedure. The purified product is then eluted and
neutralized.

The components
of the AbSelect kit are fully compatible with the Lightning-Link™ antibody labeling system
(available separately), which allows the purified antibody to be rapidly
conjugated with a wide variety of labels.

腹水、血清純化抗體à AbSelect 血清抗體------純化系統

適合應用: 腹水及血清產製之抗體。純化來自培養液、腹水及血清之抗體，移除殘留之amino group。純化後之抗體，可直接進行抗體標誌(lightning_link)、或奈米金標誌(innovaCoat)之試驗。

AbSelect Serum Antibody Purification System

Antibodies
generated from ascites fluid and serum are often supplied as crude
formulations. Both ascites fluid and serum contain many substances that
interfere with antibody labeling reactions. Successful labeling therefore
requires antibodies to be purified prior to labeling. The AbSelect Serum system
is a fast and simple method to purify antibodies from these types of media.

AbSelect Serum
is prepared by coupling highly purified protein A to agarose beads which can be
used to purify IgG fractions from ascites fluid and serum. The method involves
the capture of the antibody on the AbSelect Serum resin and the removal of
unwanted substances using a simple wash procedure. The purified product is then
eluted and neutralized.

The components
of the AbSelect Serum purification system are fully compatible with the
Lightning-Link™ antibody labeling kits (available separately),
which allows the purified antibody to be immediately labeled with an enzyme,
fluorescent protein or fluorescent dye, with a hands-on time of under 30
seconds.

腹水、血清純化抗體à AbSelect TCS組織培養液抗體------純化系統

適合應用: 融合瘤組織培養液(TCS)產製之抗體。純化來單株細胞產製之抗體，移除培養液中殘留之amino group。純化後之抗體，可直接進行抗體標誌(lightning_link)、或奈米金標誌(innovaCoat)之試驗。

AbSelect TCS Antibody Purification System

Antibodies are
often generated from hybridoma cell lines and supplied in tissue culture
supernatant (TCS). TCS itself contains many substances that interfere with
antibody labeling reactions. Successful labeling therefore requires antibodies
to be purified prior to labeling. The AbSelect TCS system is a fast and simple
method to purify these antibodies.

AbSelect TCS
is prepared by coupling highly purified protein A to agarose beads which can be
used to purify IgG fractions from hybridoma supernatants. The method involves
the capture of the antibody on the AbSelect TCS resin and the removal of
unwanted substances using a simple wash procedure. The purified product is then
eluted and neutralized.

The components
of the AbSelect TCS are fully compatible with the Lightning-Link™ antibody labeling kits (available separately),
which allows the purified antibody to be immediately labeled with an enzyme,
fluorescent protein or fluorescent dye, with a hands-on time of under 30
seconds.

抗體濃縮及Clean up系統

適合應用: 低濃度抗體之純化及濃縮。干擾酵素型HRP、Biotin、streptavidin及FITC螢光等之抗體標誌反應。移除溶液中殘留之amino group。純化後之抗體，可直接進行抗體標誌(lightning_link)、或奈米金標誌(innovaCoat)之試驗。

Antibody Concentration and Cleanup Kit

Antibodies are
sometimes only available at low concentrations and often contain low molecular
weight substances that interfere in labeling reactions with enzymes, biotin,
streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit
allows for the quick and easy concentration of antibodies and proteins. The kit
can also be used to reduce the concentration of many unwanted additives often
found in antibody formulations such as azide, glycine or tris.

The antibody
clean up kit method utilizes a simple spin column to easily and quickly remove
excess buffer from the antibody thereby providing a more concentrated antibody
solution. The clean up kit also allows the experimenter to perform a simple
buffer exchange to transfer the antibody into a more favorable conjugation
buffer.

DIY抗體標誌螢光(FITC,PE,Cy3,Cy5,Cy7 dye)

注意事項

請確認抗體的保存條件。傳統市售的抗體標誌套組，在使用前皆需dialysis純化，將抗體保存的物質移除。Lightning-link抗體標誌套組在大部份的保存條件下，不需要再dialysis的步驟，例如一般salts (e.g. NaCl)、sugars(e.g. sucrose)、及chelators (e.g. EDTA)不會影響標誌的活性。大部的市售的抗體保存在1%BSA、0.5%Azide及 0.5% Gelatin 輕微或幾乎不會影響標誌的活性。在少數情況下，若是您購買的抗體保存在amino acids(e.g. glycine)、blockers(e.g.
ethanolamine)及thiols(DTT, mercaptoethanol)情況下，記得純化您的抗體，(abselect 抗體純化套組) 避免抑制您的標誌活性了!

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【連絡信箱】taiding.biotech@msa.hinet.net

【台北公司】02-86609496 【台中公司】0935-229803 陳俊豪

【公司網址】 www.biopioneer.com.tw

【原廠網址】http://www.innovabiosciences.com

HRP抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#701-0000)     

抗體標誌directly labeling kit，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link
抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino
groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

Lightning-link
抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link
抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western
Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白
螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link
產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose
Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-Link®
Alkaline Phosphatase (AP)

Lightning-Link®
Glucose Oxidase

Lightning-Link®
Horseradish Peroxidase (HRP)

Lightning-Link®
Avidin

Lightning-Link®
Biotin

Lightning-Link®
Streptavidin

Lightning-link
酵素型抗體標誌---期刊發表:

Lightning-Link
HRP  ---期刊發表

• Measurement of MMP-9 and -12 degraded elastin (ELM)
  provides unique
  
  information on lung tissue degradation - BMC Pulmonary
  Medicine (2012)


• The carboxy-terminus of TFPIα is required for its
  interaction with factor V and Va - Journal of Thrombosis and
  Haemostasis (2012)


• Development of monoclonal antibody-based immunoassays
  for detection of Helicobacter pylori neutrophil-activating protein
  - Journal of Immunological Methods (2012)


• Sensitive quantitative assays for tau and phospho-tau
  in transgenic mouse models - Neurobiology of Aging (2012)


• A Novel System for Rapid and Cost-Effective
  Production of Detection and Diagnostic Reagents of West Nile Virus in
  Plants - Journal of Biomedicine and Biotechnology (2012)


• Porcine Reproductive and Respiratory Syndrome Virus
  Activates the Transcription of Interferon alpha/beta (IFN-α/β) in
  Monocyte-derived Dendritic Cells (Mo-DC) - Veterinary
  Microbiology (2012)


• A Human PrM Antibody That Recognizes a Novel Cryptic
  Epitope on Dengue E Glycoprotein - PLoS One (2012)


• A Conserved Tandem Cyclophilin-Binding Site in
  Hepatitis C Virus Nonstructural Protein 5A Regulates Alisporivir
  Susceptibility - Journal of Virology (2012)

• Bacitracin inhibits the reductive activity of protein
  disulfide isomerase by disulfide bond formation with free cysteines in the
  substrate-binding domain - FEBS Journal (2011)


• Dual
  Neofunctionalization of a Rapidly Evolving Aquaporin-1 Paralog Resulted in
  Constrained and Relaxed Traits Controlling Channel Function during Meiosis
  Resumption in Teleosts - Mol Biol Evol (2011)


• Comparison of the efficiency of antibody selection
  from semi-synthetic scFv and non-immune Fab phage display libraries
  against protein targets for rapid development of diagnostic immunoassays
  - Journal of Immunological Methods (2011)


• Protein biomarkers in exfoliated cells collected from
  the human rectal mucosa: implications for colorectal disease detection and
  monitoring - International Journal of Colorectal Disease
  (2011)


• Assessment
  of proteolytic degradation of the basement membrane: a fragment of type IV
  collagen as a biochemical marker for liver fibrosis - Fibrogenesis
  & Tissue Repair (2011)


• Canine specific ELISA for coagulation factor VII
  - The Veterinary Journal (2011)


• Aberrant splicing of the hRasGRP4 transcript and
  decreased levels of this signaling protein in the peripheral blood
  mononuclear cells in a subset of patients with rheumatoid arthritis
  - PLoS One (2011)


• MMP Mediated Degradation of Type VI Collagen Is
  Highly Associated with Liver Fibrosis – Identification and Validation of a
  Novel Biochemical Marker Assay - PLoS One (2011)
  
  


• Ubiquitin Fold Modifier 1 (UFM1) and Its Target
  UFBP1 Protect Pancreatic Beta Cells from ER Stress-Induced Apoptosis - PLoS One (2011)


• A novel marker for assessment of liver matrix
  remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP
  generated type I collagen neo-epitope (C1M) - Biomarkers
  (2011)


• Immunological detection of the type V collagen
  propeptide fragment, PVCP-1230, in connective tissue remodeling associated
  with liver fibrosis - Biomarkers (2011)


• Methodologic issues in the measurement of
  interleukin-16 in clinical blood samples using immunoassays -
  Cytokine (2011)


• Histidine-rich Glycoprotein Binds Fibrin(ogen) with
  High Affinity and Competes with Thrombin for Binding to the γ′-Chain
  - JBC (2011)

• Monoclonal antibody to novel cell surface epitope on
  Hsc70 promotes morphogenesis of bile ducts in newborn rat liver
  - Cell Stress and Chaperones (2010)


• Immunogenicity of attenuated measles virus engineered
  to express Helicobacter pylori neutrophil-activating protein
  - Vaccine (2010)


• Peptide Route Functionalization of ZnSe Crystals
  Preserves Activity and Structure of Proteins while Adsorption -
  The Journal of Physical Chemistry C (2010)


• Monoclonal antibody to novel cell surface epitope on
  Hsc70 promotes morphogenesis of bile ducts in newborn rat liver
  - Cell Stress and Chaperones (2010)


• Clathrin-independent
  carriers form a high capacity endocytic sorting system at the leading edge
  of migrating cells - JCB (2010)


• Regeneration of Dental-Pulp-like Tissue by
  Chemotaxis-Induced Cell Homing - Tissue Eng Part A. (2010)


• The Phosphatase Interactor NIPP1
  Regulates the Occupancy of the Histone Methyltransferase EZH2 at Polycomb
  Targets – Nucleic Acids Research (2010)

• Extinction-Reconsolidation
  Boundaries: Key to Persistent Attenuation of Fear Memories - Science
  (2009)


• Development
  of a highly sensitive ELISA for aldosterone in mouse urine: Validation in
  physiological and pathophysiological states of aldosterone excess and
  depletion - Steroids (2009)


• Contributions
  to Neutropenia from PFAAP5 (N4BP2L2), a Novel Protein Mediating
  Transcriptional Repressor Cooperation between Gfi1 and Neutrophil Elastase - Molecular and
  Cellular Biology (2009)

LifeXtend
HRP Conjugagte Stabilizer

• Sensitive quantitative assays for tau and phospho-tau
  in transgenic mouse models - Neurobiology of Aging (2012)

Lightning-Link
Biotin  ---期刊發表

• Controlled release of thymosin β4 from injected
  collagen–chitosan hydrogels promotes angiogenesis and prevents tissue loss
  after myocardial infarction - Regenerative Medicine (2012)


• Engrailed-2 (EN2): A Tumor
  Specific Urinary Biomarker for the Early Diagnosis of Prostate Cancer
  – Clinical Cancer Research (2011)


• Human Alpha Defensin 5 Expression in the Human Kidney
  and Urinary Tract - PLoS One (2011)


• Ribonuclease 7 is a potent antimicrobial peptide
  within the human urinary tract - Kidney International (2011)

• Method for Decreasing Immunogenicity ; Patent
  publication number US2011/0152505 (2010)


• Targeting
  MHC Class I Monomers to Dendritic Cells Inhibits the Indirect Pathway of
  Allorecognition and the Production of IgG Alloantibodies Leading to
  Long-Term Allograft Survival - Journal of Immunology (2010)


• Stromal
  Cell-Derived Factor-1α Activation of Tissue-Engineered Endothelial
  Progenitor Cell Matrix Enhances Ventricular Function After Myocardial
  Infarction by Inducing Neovasculogenesis - Cell Transplantation
  and Tissue Regeneration (2010)


• In Vivo
  Studies of Mutant Fibrillin-1 Microfibrils - JBC (2010)


• Serum
  insulin-like growth factor binding protein-1 (IGFBP-1) phosphorylation
  status in subjects with and without ischaemic heart disease - Artherosclerosis
  (2010)


• HER2 Phosphorylation Is
  Maintained by a PKB Negative Feedback Loop in Response to Anti-HER2
  Herceptin in Breast Cancer – PLoS Biology (2010)

• Human
  Mesenchymal Stem Cells Self-Renew and Differentiate According to a
  Deterministic Hierarchy – PLoS One (2009)


• Bovine
  lactoferrin and lactoferricin interfere with intracellular trafficking of
  Herpes simplex virus-1- Biochemie (2009)

• Functional studies of host-specific ephrin-B ligands
  as Henipavirus receptors - Virology (2008)

Lightning-Link
Avidin  ---期刊發表:

• Targeting MHC Class I Monomers
  to Dendritic Cells Inhibits the Indirect Pathway of Allorecognition and
  the Production of IgG Alloantibodies Leading to Long-Term Allograft
  Survival – Journal of Immunology (2010)

Lightning-Link
AP  ---期刊發表

• Large molecular size EDTA-resistant complexes
  containing S100A12, ERAC, in serum during inflammatory conditions
  - Scandinavian Journal of Clinical & Laboratory Investigation
  (2012)


• Broad-Spectrum Inhibition of HIV-1 by a
  Monoclonal Antibody Directed against a gp120-Induced Epitope of CD4 - PLoS One (2011)


• Fluorescent
  Labeling of Antibody Fragments Using Split GFP - PLoS One (2011)


• In Vivo
  Studies of Mutant Fibrillin-1 Microfibrils - JBC (2010)


• Microarray immunoassay for the detection of grapevine
  and tree fruit viruses - Journal of Virological Methods
  (2009)

Lightning-link 抗體標誌介紹:

(ATTO系列抗體螢光標誌套組)

產品特點

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

產品規格:

3x100ug

每次最大標誌量200ug, 一組可進行3次標誌

Lightning-link 抗體標誌介紹:

(R-PE抗體螢光標誌套組, 貨號#703-0010)

產品特點

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

產品規格:

1.      
3x100ug (貨號703-0010)

每次最大標誌量200ug, 一組可進行3次標誌

2.      
3x10ug (貨號703-0030)

每次最大標誌量20ug, 一組可進行3次標誌

3.      
1x100ug (貨號703-0005)

每次最大標誌量00ug, 一組可進行1次標誌

4.      
1x1mg (貨號703-0015)

每次最大標誌量2ug, 一組可進行1次標誌

Label

Flow cytometry data comparing direct and indirect labeling:

Indirect labeling with CD45RC supernatant	Indirect labeling with CD45RC purified Ab	Direct labeling with Lightning-Link CD45RC-PE Purified Ab

3x10µg Protocol
1x100µg 3x100µg 1x1mg Protocol
MSDS

 

Lightning-link 抗體標誌介紹:

(APC抗體螢光標誌套組, 貨號#705-0010)

產品特點

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

產品規格:

3x100ug

每次最大標誌量200ug, 一組可進行3次標誌

Label

Allophycocyanin is a large 105kDa fluorescent phycobiliprotein commonly used in FACS analysis. It absorbs at 650nm and emits at 662nm.

Lightning-link 抗體標誌介紹:

(FITC抗體螢光標誌套組, 貨號#707-0010)

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

綠色螢光-FITC抗體標誌套組

(螢光excitation494nm/emission520nm)

產品規格:

3x100ug

(每次最大標誌量200ug, 一組可進行3次標誌)

Label

Fluorescein is a fluorophore widely used in biological analysis, with a pH dependent absorption and emission spectrum. In water it shows an absorption maximum at 494 nm and an emission maximum of 520 nm

產品特點

30 seconds hands-on timeNo separation stepsNo lossesScalable technology

Easy to use antibody labeling kit Antibody recovery is 100% Applicable to Western Blotting, ELISA, Immunohistochemistry, Immunofluorescence, and FACS analysis From 10µg up to 5mg

Lightning-link 抗體標誌介紹:

(藍色螢光-Atto 390貨號#730-0010)

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

藍色螢光-Atto 390抗體標誌套組

(螢光excitation390nm/emission479nm)

產品規格:

3x100ug

(每次最大標誌量200ug, 一組可進行3次標誌)

Label
Atto390 is one of a new generation of fluorescent labels with a coumarin structure. It has a strong absorption at 390nm, high fluorescence at 479nm (extinction coefficient 2.4 x10⁴ cm^-1M^-1) and high quantum yield.

產品特點