太鼎生物科技/太鼎食安科技

ELISA Kit for Immunoglobulin M (IgM)

 

Specificity

This assay has high sensitivity and excellent
specificity for detection of Immunoglobulin M (IgM).

No significant cross-reactivity or interference between Immunoglobulin M (IgM)
and analogues was observed.

Recovery

Matrices listed below were spiked with certain level
of recombinant Immunoglobulin M (IgM) and the recovery rates were calculated by
comparing the measured value to the expected amount of Immunoglobulin M (IgM)
in samples.

Precision

Intra-assay Precision (Precision within an assay): 3
samples with low, middle and high level Immunoglobulin M (IgM) were tested 20
times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle
and high level Immunoglobulin M (IgM) were tested on 3 different plates, 8
replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing
samples spiked with appropriate concentration of Immunoglobulin M (IgM) and
their serial dilutions. The results were demonstrated by the percentage of
calculated concentration to the expected.

Stability

The stability of kit is determined by the loss rate
of activity. The loss rate of this kit is less than 5% within the expiration
date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab
conditions, especially room temperature, air humidity, incubator temperature
should be strictly controlled. It is also strongly suggested that the whole
assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Assay procedure summary

1. Prepare all reagents, samples and standards;

2. Add 100µL standard or sample to each well. Incubate 2 hours at 37^oC;

3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37^oC;

4. Aspirate and wash 3 times;

5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37^oC;

6. Aspirate and wash 5 times;

7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37^oC;

8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle

The test principle applied in this kit is Sandwich
enzyme immunoassay. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Immunoglobulin M (IgM). Standards or
samples are then added to the appropriate microtiter plate wells with a
biotin-conjugated antibody specific to Immunoglobulin M (IgM). Next, Avidin conjugated
to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
After TMB substrate solution is added, only those wells that contain
Immunoglobulin M (IgM), biotin-conjugated antibody and enzyme-conjugated Avidin
will exhibit a change in color. The enzyme-substrate reaction is terminated by
the addition of sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of
Immunoglobulin M (IgM) in the samples is then determined by comparing the O.D.
of the samples to the standard curve.