太鼎生物科技/太鼎食安科技

膽固醇呈色/螢光分析套組

Cholesterol/Cholesteryl Ester Quantitation Colorimetric/Fluorometric Kit

Sensitive, Colorimetric & Fluorometric Assay



Catalog#: K603-100  | Size: 100 assays

Kit
Summary:

• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum,
as well as many other biological fluids

• Species reactivity- Mammalian

• Application- The assay detects total cholesterol (cholesterol and cholesteryl
esters) in the presence of cholesterol esterase or free cholesterol in the
absence of cholesterol esterase in the reaction.

Features
& Benefits:

• Simple procedure; takes ~60 minutes

• Fast and convenient

Kit
components:

• Cholesterol Assay Buffer

• Cholesterol Probe (in DMSO, anhydrous)

• Enzyme Mix (lyophilized)

• Cholesterol Esterase (lyophilized)

• Cholesterol Standard (2 µg/µl)

Description:


The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for
sensitive quantification of free cholesterol, cholesteryl esters, or both by
colorimetric or fluorometric methods. Majority of the cholesterol in blood is
in the form of cholesteryl esters which can be hydrolyzed to cholesterol by
cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to
yield H₂O₂which
reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and
fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol
(cholesterol and cholesteryl esters) in the presence of cholesterol esterase or
free cholesterol in the absence of cholesterol esterase in the reaction.
Cholesteryl ester can be determined by subtracting the value of free
cholesterol from the total (cholesterol plus cholesteryl esters).