ALT/AST/SGOT活性呈色分析套組 (405nm)





Aspartate Aminotransferase (AST or SGOT) Activity
Colorimetric Assay Kit  




 


ALT/AST/SGOT活性呈色分析套組





Highly Sensitive Assay, HTS





Catalog#: K753-100  | Size:100
assays





產品特點







 




產品特點




Kit
Summary:


• Detection method- Absorbance (450 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum,
as well as many other biological fluids etc.

• Species reactivity- Mammalian

• Application- This assay detects AST/ SGOT with a detection limit of 10 mU per
well.




Features
& Benefits:


• Simple procedure

• Fast, reliable and convenient

• Sensitive assays for measuring AST/ SGOT activity in various biological
samples and the kit is also suitable for high throughput analyses.




產品內容物




Kit
components:


• AST Assay Buffer

• AST Enzyme Mix

• Developer

• AST Substrate

• Glutamate Standard ( 0.1M )

• AST Positive Control




產品介紹




Description:Aspartate
aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT)
is a transaminase (EC 2.6.1 .1) similar
to the more liver specific alanine transaminase (ALT). Although commonly
included clinically as part of a diagnostic liver function test, AST has a
broader clinical utility since it may also be elevated in diseases affecting
other organs, such as the heart or muscles in myocardial infarction, also in
acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease,
musculoskeletal diseases and trauma. It catalyzes the reaction:




Aspartate
+
-Ketoglutarate    
 Oxaloacetate + Glutamate  




 Diagnostically,
it is almost always measured in units/liter (U/L). In BioVision’s AST Assay
Kit, an amino group is transferred of from aspartate to
-ketoglutarate.
The products of this reversible transamination reaction are oxaloacetate and
glutamate. The glutamate is detected in a reaction that concomitantly converts
a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid,
simple, sensitive and reliable test suitable as a high throughput activity
assay of AST with a detection limit of 10 mU per well.




Storage
Conditions:
-20°C




 




【經銷商】太鼎生物科技有限公司




 【連絡人】許虹宜 0920-312382 Hung-Yi Hsu




E-mailtaiding.biotech@msa.hinet.net




【公司電話】02-86609496【公司傳真】02-86609342


 


【公司網址】www.biopioneer.com.tw





【原廠網址】www.biovision.com.tw
 





 


 




 








 



 



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