【GST呈色分析套組 (380nm)】訂購貨號# K263-100 。麩胺基硫轉移酵素分析套組。100個分析。GST Colorimetric Activity Assay Kit @ 太鼎生物科技/太鼎食安科技

GST呈色分析套組 (380nm)

GST Colorimetric Activity Assay Kit

麩胺基硫轉移酵素分析套組

Catalog#: K263-100 | Size:
100 assays

產品特點

Kit
Summary:

• Detection method- Absorbance (380 nm)

• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum,
as well as many other biological fluids

• Species reactivity- Mammalian

• Applications- The kit can detect GST activity in crude cell lysate or
purified protein fraction, and also quantitate GST-tagged fusion protein.

Features
& Benefits:

• Simple procedure; takes only ~1-1.5 hours

• Fast and convenient

產品內容物

•
GST Assay Buffer

• GST Substrate (CDNB)

• Glutathione (GSH, lyophilized)

• GST Positive Control (0.625 µg/µl)

產品介紹

Description:

Glutathione S-transferase (GST) is a family of enzymes that play an important
role in detoxification of xenobiotics. GST catalyzes the formation of the thiol
group of glutathione to electrophilic xenobiotics. It utilizes glutathione to
scavenge potentially toxic compounds including those produced as a result of
oxidative stress and is part of the defense mechanism against the mutagenic,
carcinogenic and toxic effects of such compounds. The GST Colorimetric Activity
Assay Kit is based upon the GST-catalyzed reaction between GSH and the GST
substrate, CDNB (1-chloro-2,4-dinitrobenzene, which has the broadest range of
isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). Under
certain conditions, the interaction between glutathione and CDNB is totally
dependent on the presence of active GST. The GST-catalyzed formation of GS-DNB
produces a dinitrophenyl thioether which can be detected by spectrophotometer
at 340 nm. One unit of GST activity is defined as the amount of enzyme
producing 1 µmol of GS-DNB conjugate/min under the conditions of the assay. The
kit can detect GST activity in crude cell lysate or purified protein fraction,
and also quantitate GST-tagged fusion protein. Detect limit: Active GST < 4
mU.