【H7N9 HA蛋白質 (HA1+HA2) 】#40103-V08H4。Influenza A H7N9 (A/Anhui/1/2013) HA / Hemagglutinin Protein－太鼎生物科技/太鼎食安科技

Anti-H7N 9 HA蛋白質 (HA1+HA2)

Influenza
A H7N9 (A/Anhui/1/2013) HA / Hemagglutinin Protein Datasheet

H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein

H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein Product Information

Synonym :	HA
Protein Construction:	A DNA sequence encoding the Influenza A virus (A/Anhui/1/2013(H7N9)) hemagglutinin (AGJ51953.1) (Met1-Val524) (cleavage between Arg339 and Gly440, HA1+HA2) was expressed with a C-terminal polyhistidine tag.
Source:	Influenza A virus
Expression Host:	Human Cells

H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein
QC Testing

Purity:	(53.8+44.7) % as determined by SDS-PAGE	SDS-PAGE: H7N 9 HA protein
Endotoxin:	< 1.0 EU per μg of the protein as determined by the LAL method
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Asp 19
Molecular Mass:	The recombinant hemagglutinin of Influenza A virus (A/Anhui/1/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 43 and 27 kDa in SDS-PAGE under reducing conditions.
Formulation:	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Amino Acid Sequence

DKICLGHHAV SNGTKVNTLT ERGVEVVNAT ETVERTNIPR ICSKGKRTVD
LGQCGLLGTI TGPPQCDQFL EFSADLIIER REGSDVCYPG KFVNEEALRQ ILRESGGIDK EAMGFTYSGI
RTNGATSACR RSGSSFYAEM KWLLSNTDNA AFPQMTKSYK NTRKSPALIV WGIHHSVSTA EQTKLYGSGN
KLVTVGSSNY QQSFVPSPGA RPQVNGLSGR IDFHWLMLNP NDTVTFSFNG AFIAPDRASF LRGKSMGIQS
GVQVDANCEG DCYHSGGTII SNLPFQNIDS RAVGKCPRYV KQRSLLLATG MKNVPEIPKG RGLFGAIAGF
IENGWEGLID GWYGFRHQNA QGEGTAADYK STQSAIDQIT GKLNRLIEKT NQQFELIDNE FNEVEKQIGN
VINWTRDSIT EVWSYNAELL VAMENQHTID LADSEMDKLY ERVKRQLREN AEEDGTGCFE IFHKCDDDCM
ASIRNNTYDH SKYREEAMQN RIQIDPVKLS SGYKDVAHHH HHHHHHH

H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein Usage Guide

Storage:	Store it under sterile conditions at -70℃ . It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein Related Products & Topics

Related Areas:

Proteins:

Antibodies:

H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein Description

H7N9 is a subtype of Influenza
virus A. On April 1, 2013, the World Health Organization (WHO) first reported 3
human infections with a new influenza A (H7N9) virus in China . Since
then, additional cases have been reported. This new H7N9 virus is an avian
(bird) influenza (flu) virus. Influenza (flu) is a respiratory infection in
mammals and birds. The virus is divided into three main types (Influenza A, Influenza
B, and Influenza C), which are distinguished by differences in two major
internal proteins (hemagglutinin (HA) and neuraminidase (NA).

Influenza A is further divided
into subtypes based on differences in the membrane proteins hemagglutinin (HA)
and neuraminidase (NA), which are the most important targets for the immune
system. The notation HhNn is used to refer to the subtype comprising the hth
discovered Hemagglutinin (HA) protein and the nth discovered neuraminidase (NA)
protein. The influenza viral Hemagglutinin (HA) protein is a homo trimer with a
receptor binding pocket on the globular head of each monomer.

The influenza virus
Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or
hemagglutinin precursor protein. For viral activation, hemagglutinin precursor
protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a
specific site, normally coded for by a single basic amino acid (usually
arginine) between the HA1 and HA2 domains of the protein. After cleavage, the
two disulfide-bonded protein domains produce the mature form of the protein
subunits as a prerequisite for the conformational change necessary for fusion
and hence viral infectivity.

References

1.
Barman S. et al., 2000, J Virol. 74: 6538-45.

2.
Suzuki T. et al., 2005, J Virol. 79: 11705-15.

3.
Shinya K. et al., 2006, Nature. 440 (7083): 435-6.

4.
Marjuki H. et al., 2006, J Biol Chem. 281: 16707-15.

5.
Christophe F. et al., 2009, Science. 324: 1557-61.

6.
Von Itzstein M. 2007, Nat Rev Drug Discov. 6: 967-74.