A型流感H5N1蛋白質100ug-His





(A/Common
magpie/Hong Kong/2256/2006) HA1 Protein





 























Synonym :




HA1




Protein Construction:




A DNA sequence encoding
the N-terminal segment ( Met 1-Glu 340 ) of the influenza hemagglutinin
(A/Common magpie/Hong Kong/2256/2006 (H5N1)) (ABJ96777.1), termed as HA1, was
fused with a C-terminal polyhistidine tag




Source:




Influenza A H5N1




Expression Host:




Human Cells








 




H5N1 (A/Common magpie/Hong
Kong/2256/2006) HA1 Protein QC Testing


































Purity:




> 97 % as determined by SDS-PAGE




SDS-PAGE:

 



H5N1 HA1 protein





Endotoxin:




< 1.0 EU per μg of the protein as determined by the LAL method




Stability:




Samples are stable for up to twelve months from date of receipt at -70




Predicted N terminal:




Asp 17




Molecular Mass:




The secreted recombinant
HA1 subunit influenza A H5N1 (A/Common magpie/Hong Kong/2256/2006 (H5N1)) HA
comprises 335 amino acids and has a predicted molecular mass of 38 kDa. As a
result of glycosylation, it migrates as an approximately 50-55 kDa band in
SDS-PAGE under reducing conditions




.








H5N1 (A/Common magpie/Hong
Kong/2256/2006) HA1 Protein Usage Guide

















Storage:




Store it under sterile conditions at -70. It is recommended that
the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw
cycles
.




Reconstitution:




A hardcopy of COA with reconstitution instruction is sent along with the
products. Please refer to it for detailed information






 





Molecular
Mass:





The
secreted recombinant HA1 subunit influenza A H5N1 (A/Common magpie/Hong
Kong/2256/2006 (H5N1)) HA comprises 335 amino acids and has a predicted
molecular mass of 38 kDa. As a result of glycosylation, it migrates as an
approximately 50-55 kDa band in SDS-PAGE under reducing conditions





 





Protein Description





Influenza (flu) is a respiratory infection in
mammals and birds. This virus is divided into three main types (A, B and C).
Influenza A is found in a wide variety of bird and mammal species and is
further divided into subtypes based on differences in the membrane proteins
hemagglutinin (HA) and neuraminidase (NA). Influenza A virus subtype H5N1, also
known as "bird flu", A(H5N1) or simply H5N1, is a subtype of the
Influenza A virus which can cause illness in humans and many other animal
species. H5N1 is easily transmissible between birds facilitating a potential
global spread of H5N1. It is mainly spread by domestic poultry, both through
the movements of infected birds and poultry products and through the use of
infected poultry manure as fertilizer or feed. Humans with H5N1 have typically
caught it from chickens, which were in turn infected by other poultry or waterfowl.





Hemagglutinin (HA) is a single-pass type I
integral membrane glycoprotein from the influenza virus, and comprises over 80%
of the envelope proteins present in the virus particle. The HA is a trimer with
a receptor binding pocket on the globular head of each monomer. In natural
infection, inactive HA is matured into HA1 and HA2 outside the cell by one or
more trypsin-like, arginine-specific endoprotease secreted by the bronchial
epithelial cells. Binding of HA to sialic acid-containing receptors on the
surface of its target cell brings about the attachment of the virus particle to
the cell and forms a endosome. Low pH in endosomes induce an irreversible
conformational change in HA2, releasing the hydrophobic portion “fusion
peptide”. After which, virus penetrates the cell and pours its contents
including the RNA genome into the cytoplasm mediated by fusion of the
endocytosed virus particle’s own membrane and the endosomal membrane.
Hemagglutinin plays a major role in the determination of host range restriction
and virulence.








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