太鼎生物科技/太鼎食安科技

Biotin抗體標誌套組

Lightning-Link® Biotin  

Lightning-Link® kits

Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.

The Lightning-Link® range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link® conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.

Biotin Label

The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. The biotin in the kit has an extended linker to facilitate molecular interactions.

Lightning-Link® Biotin has been optimised for two separate applications. Type A is intended for assays in which a streptavidin-labeled detection reagent will be used, whilst Type B is optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).

Click here for other labels available in the Lightning-Link® range.

Manufacturing

The technology is fully scalable (μg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.

Manufacturing

The technology is fully scalable (μg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.

Type A - 3 reactions (up to 20ug scale) (704-0030)

Type A - 3 reactions (up to 200ug scale) (704-0010)

Type A - 1 reaction (up to 2mg scale) (704-0015)

Type B - 3 reactions (up to 20ug scale) (715-0030)

Type B - 3 reactions (up to 200ug scale) (715-0010)

Type B - 1 reaction (up to 2mg scale) (715-0015)

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References:

Lightning-Link Biotin

• RANKL targeted peptides inhibit osteoclastogenesis and attenuate adjuvant induced arthritis by inhibiting NF-κB activation and down regulating inflammatory cytokines - Chemico-Biological Interactions (2013)
  
• Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk - Journal of Immunological Methods (2012)
  
• Using Phage and Yeast Display to Select Hundreds of Monoclonal Antibodies: Application to Antigen 85, a Tuberculosis Biomarker - PLoS One (2012)
  
• Epitope recognition patterns of thyroglobulin antibody in sera from patients with Hashimoto's thyroiditis on different thyroid functional status - Journal of Translational Immunology (2012)
  
• Controlled release of thymosin β4 from injected collagen–chitosan hydrogels promotes angiogenesis and prevents tissue loss after myocardial infarction - Regenerative Medicine (2012)
  
• Engrailed-2 (EN2): A Tumor Specific Urinary Biomarker for the Early Diagnosis of Prostate Cancer – Clinical Cancer Research (2011)
  
• Human Alpha Defensin 5 Expression in the Human Kidney and Urinary Tract - PLoS One (2011)
  
• Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract - Kidney International (2011)
  
• Method for Decreasing Immunogenicity ; Patent publication number US2011/0152505 (2010)
  
• Targeting MHC Class I Monomers to Dendritic Cells Inhibits the Indirect Pathway of Allorecognition and the Production of IgG Alloantibodies Leading to Long-Term Allograft Survival - Journal of Immunology (2010)
  
• Stromal Cell-Derived Factor-1α Activation of Tissue-Engineered Endothelial Progenitor Cell Matrix Enhances Ventricular Function After Myocardial Infarction by Inducing Neovasculogenesis - Cell Transplantation and Tissue Regeneration (2010)
  
• In Vivo Studies of Mutant Fibrillin-1 Microfibrils - JBC (2010)
  
• Serum insulin-like growth factor binding protein-1 (IGFBP-1) phosphorylation status in subjects with and without ischaemic heart disease - Artherosclerosis (2010)
  
• HER2 Phosphorylation Is Maintained by a PKB Negative Feedback Loop in Response to Anti-HER2 Herceptin in Breast Cancer – PLoS Biology (2010)
  
• Human Mesenchymal Stem Cells Self-Renew and Differentiate According to a Deterministic Hierarchy – PLoS One (2009)
  
• Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1- Biochemie (2009)
  
• Functional studies of host-specific ephrin-B ligands as Henipavirus receptors - Virology (2008)

  [供應廠商】太鼎生物科技有限公司

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【E-mail】taiding.biotech@msa.hinet.net

【臺北公司】02-86609496

【相關網址】www.biopioneer.com.tw

【原廠網址】www.innovabiosciences.com