---------凝膠法 (Gel clot Endotoxin Assay kit)產品介紹----

內毒素檢測試劑(LAL Reagent) Gel-Colt(凝膠法) 鱟試劑與內毒素反應會產生凝膠，利用凝膠的生成與否來偵測內毒素的方法，為最早發展出來的LAL測試方法。

endotoxin_assay_kit

Genesript提供ToxinSensor 凝膠法 (Gel clot Endotoxin Assay kit)和終點顯色法(Chromogenic LAL Endotoxin Assay)，兩種內毒素檢測試劑盒，可以對人或動物的注射用藥、生物製品及醫療設備的終端產品中的內毒素進行檢測。

內毒素檢測試劑---

Genescript 提供二種型式，之內毒素檢測試劑。

ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (終點顯色法)

訂購貨號: Cat. No. L00350

產品名稱: ToxinSensor™ Chromogenic LAL
Endotoxin Assay Kit

靈敏度: Max Sensitivity: 0.005 EU/ml, or less

使用儀器: color spectrophotometer;

ToxinSensor™ Gel clot Endotoxin Assay Kit (凝膠法)

訂購貨號: Cat. No. L00351

產品名稱: ToxinSensor™ Gel clot Endotoxin
Assay Kit

靈敏度: 0.25 EU/ml

使用儀器: 37°C water bath

ToxinSensorTM凝膠法內毒素檢測試劑盒

ToxinSensor™ Gel clot Endotoxin Assay Kit (凝膠法)

訂購貨號: Cat. No. L00351

使用儀器: 37°C water bath

優點

1小時內完成所有過程

高重複性

靈敏度為0.25 EU/ml

試劑隨時可用，並附有不含內毒素的槍頭、試管

簡單省時的過程

每一個測試應該包括一個陽性對照和一個陰性對照 (LAL試劑)。應用實例如下：

1. 樣品：1 mg/ml的蛋白質A，被置於pH 7.4的磷酸緩衝液中。蛋白質A是由Ni-NTA樹脂純化大腸桿菌得到。

2. 按照稀釋比例1: 200,000，1: 400,000，1: 800,000在LAL試劑水中稀釋。

3.該測試是在上述條件下進行，得到的結果如下：

4.初步判斷該樣品的內毒素在50,000到100,000 EU/ml之間

產品規格

ToxinSensorTM 凝膠法內毒素檢測試劑盒1 kit (40次)L00351

Endotoxin Assay Kits

Endotoxin test is the most critical quality control test required by the FDA for all drugs in their final stages of formulation. Endotoxins are invariably associated with every gram-negative bacteria, so they cause severe reactions in humans and animals and retain high toxic activity even present at low concentration. In addition, endotoxins are suspected to play an important role in the occurrence and development of many different diseases.

GenScript strongly recommends innovative detection technique to detect endotoxin. GenScript's Endotoxin Assay Kits can be well used in in vitro end-product endotoxin test for human and animal parenteral drugs, biological products, and medical devices.

Key Features

Good linearity and good reproducibility
Ready-to-use reagents and materials, such as tips, endotoxin-free tubes,etc.
High sensitivity and broad application range
Competitive price

ToxinSensor^TM Chromogenic LAL Endotoxin Assay Kit: The kit utilizes a modified Limulus Amebocyte Lysate and a synthetic color producing substrate to quantitatively detect endotoxin chromogenically in a broad range (0.005–1 EU/ml).

Note:GenScript has launched a new size (32 rxns) with reasonable price on 03/08/2010.Related components have also been updated!

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Ordering Information

Cat. No.

Product Name

Size

Price

Quantity

Technical Advantages:

Under the standard conditions, the absorbance at 545 nm shows a linear relationship with the concentration in range of both 0.01 - 0.1 EU/ml and 0.1 - 1 EU/ml. Plot the absorbance for the four standards on the x-axis, the corresponding endotoxin concentration in EU/ml on the y-axis. Draw a best-fit straight line among these points and determine endotoxin concentrations of samples graphically.

Examples using following data:

Vial No.	Sample	Absorbance at 545 nm	Δ Absorbance
1	LAL reagent water (Blank)	0.035	-
2	0.01 EU/ml Standard	0.088	0.053
3	0.025 EU/ml Standard	0.133	0.098
4	0.05 EU/ml Standard	0.217	0.182
5	0.1 EU/ml Standard	0.427	0.392

If the mean absorbance of a sample is x, the endotoxin concentration of sample will be 0.2618x – 0.0012 EU/ml. All incubations were performed for 45 min. Above is only an example curve show, and the OD values of standards may be different with different assays.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【臺北公司】02-86609496 【台中公司】0935-229803

【相關網址】 www.biopioneer.com.tw

相關內毒素檢測試劑

---------終點顯色法(Chromogenic LAL Endotoxin Assay)介紹----

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