太鼎生物科技/太鼎食安科技

---------終點顯色法(Chromogenic LAL Endotoxin Assay)產品介紹----

內毒素檢測試劑(LAL Reagent) Gel-Colt(凝膠法) 鱟試劑與內毒素反應會產生凝膠，利用凝膠的生成與否來偵測內毒素的方法，為最早發展出來的LAL測試方法。

Genesript提供ToxinSensor 凝膠法 (Gel clot Endotoxin Assay kit)和終點顯色法(Chromogenic LAL Endotoxin Assay)，兩種內毒素檢測試劑盒，可以對人或動物的注射用藥、生物製品及醫療設備的終端產品中的內毒素進行檢測。

內毒素檢測試劑

Genescript 提供二種型式，之內毒素檢測試劑。

ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (終點顯色法)

訂購貨號: Cat. No. L00350

產品名稱: ToxinSensor™ Chromogenic LAL
Endotoxin Assay Kit

靈敏度: Max Sensitivity: 0.005 EU/ml, or less

使用儀器: color spectrophotometer;

ToxinSensor™ Gel clot Endotoxin Assay Kit (凝膠法)

訂購貨號: Cat. No. L00351

產品名稱: ToxinSensor™ Gel clot Endotoxin
Assay Kit

靈敏度: 0.25 EU/ml

使用儀器: 37°C water bath

ToxinSensorTM終點顯色法內毒素檢測試劑盒

一種快速高敏感的終點顯色LAL檢測

ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (終點顯色法)

使用儀器: color spectrophotometer;

優點

ToxinSensorTM終點顯色法內毒素檢測試劑盒的作用原理是在LAL測試中用內毒素催化酶原的活性。這樣可以分離出無色物質並產生有色的最終產物，該產物可以在分光光度計中545 nm光照處被檢測到，並與標準物質曲線進行對照。

高重複性和高線性相關

我們分別用X公司和genescript公司終點顯色法內毒素檢測試劑盒對濃度範圍為0.1-1 EU/ml的內毒素標準品進行檢測，結

果如下：

產品規格

ToxinSensorTM 終點顯色法內毒素檢測試劑盒1 kit (16次) L00350C

ToxinSensorTM 終點顯色法內毒素檢測試劑盒1 kit (32次) L00350

 ToxinSensor^TM Gel Clot Endotoxin Assay Kit: It is a qualitative test for Gram-negative bacterial endotoxin. Limulus Amebocyte Lysate as supplied is to be reconstituted with LAL Reagent Water and then mixed in equal parts with the solution being tested. After incubation, and in the presence of endotoxin, gelation occurs; in the absence of endotoxin, gelation does not occur.

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Ordering Information

Limulus Amebocyte Lysate (LAL) as supplied is to be reconstituted with sample or control directly. There is no need to reconstitute the system with LAL Reagent Water before use. After incubation for 1 hour, and in the presence of endotoxin, gelation occurs; in the absence of endotoxin, gelation does not occur.

GenScript supply a series of ToxinSensor™ Single Test Kits with different sensitivity (0.015 EU/ml, 0.03 EU/ml, 0.06 EU/ml, 0.125 EU/ml and 0.25 EU/ml).

When using the ToxinSensor™ Single Test Kit, you just need to add 200µl of sample or control to LAL, then, wait for the required incubation time to allow for gel to be formed.

Each assay should include both a positive control and a negative control (LAL Reagent Water). Following are application example:

• Sample: Protein A, 1 mg/ml in PBS, pH 7.4. It is purified from E. coli sonicate by Ni-NTA resin.
• Dilution by LAL water: 1: 200,000, 1: 400,000, 1: 800,000
• The test is performed as described above and the assay result is:

• Endotoxin concentration in this sample is: from 200,000×0.25 to 400,000×0.25, that is, from 50,000 to 100,000 EU/ml.

相關內毒素檢測試劑

 ---------凝膠法 (Gel clot Endotoxin Assay kit)產品---

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【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【臺北公司】02-86609496 【台中公司】0935-229803

【相關網址】 www.biopioneer.com.tw