太鼎生物科技/太鼎食安科技

GTPase分析套組產品介紹 

Innova Biosciences 提供之GTPase分析套組，可探討酵素活性是否扮演transport、signal transduction,protein biosynthesis及cell differentiation之角色。應用於GTPase活性分析。提供high-throughput screen 大量GTPase inhibitor。可建立快速藥物篩選系統。內附有GTPase inhibitor。GTPase酵素活性分析，採用超高靈敏度Malachite green磷酸根呈色方法，克服傳統呈色方法,低靈敏度限制。測量目標樣本中， GTPases酵素活性

GTPases酵素活性之分析

產品特點

1. 提供high-throughput screen 大量GTPase inhibitor

2. GTPase酵素分析套組，包括stabilizing solution，可避開acid labile substrate所造成的高背景值，其背景值很低(
1mM )。

3. 其分析波段為600~660nm，避開一般colored compounds之干擾。

4. Pi-dye複合物極為穩定，其訊號強度可維持數小時之久。

5. Superior呈色系統。採用Malachite Green磷酸根呈色方式，呈色靈敏度比傳統呈色方法高，克服低靈敏度的限制。

6. 適用於大部份的assay buffer。

7. 蛋白質濃度高時，不會抑制顏色的形成。

-------------------------------

產品內容物

1 x 10ml of PiColorLock™ Gold* (1 x 25ml)

1 x 0.25ml of Accelerator (1 x 0.5ml)

1 x 5ml of Stabiliser (1 x 10ml)

1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)

1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)

1 x 5ml of 0.1mM Pi standard (1 x 10ml)

更多參考內容: 原廠連結: http://www.innovabiosciences.com/

訂購貨號# 602-0120,196 assays (靈敏度4~40uM)

訂購貨號# 602-0121,480 assays (靈敏度4~40uM)

訂購貨號# 602-0122,480*5 assays (靈敏度4~40uM)

GTPase酵素活性分析套組---文獻發表

GTPase酵素活性分析套組

The selenocysteine-specific elongation factor
contains a novel and multi-functional domain - The
Journal of Biological Chemistry (2012)

Tubulin nucleotide status controls Sas-4-dependent
pericentriolar material recruitment - Nature Cell
Biology (2012)

The Mitochondrial Phosphatase PGAM5 Functions at
the Convergence Point of Multiple Necrotic Death Pathways
- Cell (2012)

Leucyl-tRNA Synthetase Is an Intracellular
Leucine Sensor for the mTORC1-Signaling Pathway - Cell
(2012)

• ArfGAP1 Is a GTPase Activating Protein for
  LRRK2: Reciprocal Regulation of ArfGAP1 by LRRK2 - Journal
  of Neuroscience (2012)

GTPase Activity and Neuronal Toxicity of
Parkinson's Disease–Associated LRRK2 Is Regulated by ArfGAP1
- PLoS Genetics (2012)

AIP1 Functions as Arf6-GAP to Negatively
Regulate TLR4 Signaling - JBC (2010)

GTPase activity plays a key role in the
pathobiology of LRRK2 - PLoS Genetics (2010)

Role of the
G-protein and tyrosine kinase—Rho/ROK pathways in
15-hydroxyeicosatetraenoic acid induced pulmonary vasoconstriction in
hypoxic rats - J Biochem (2010)

AIP1 Functions as Arf6-GAP to Negatively
Regulate TLR4 Signaling - JBC (2010)

Identification and Characterization of a
Leucine-Rich Repeat Kinase 2 (LRRK2) Consensus Phosphorylation Motif
- PLoS One (2010)

Speckled-like Pattern in the Germinal Center
(SLIP-GC), a Nuclear GTPase Expressed in Activation-induced
Deaminase-expressing Lymphomas and Germinal Center B Cells
- JBC (2009)

High-level production and characterization of a
G-protein coupled receptor signaling complex -
FEBS Journal (2009)

Structure of ERA in
complex with the 3′ end of 16S rRNA: Implications for ribosome biogenesis
-
PNAS (2009)

G Protein-Coupled Receptor-Promoted Trafficking
of Gβ1γ2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by
Gβ1γ2-Rab11a Interaction – Molecular Biology of
the Cell (2008)

Characterization
of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA
Modification - Molecular and Cellular Biology
(2008)

Comparative real-time PCR and enzyme analysis of
selected gender-associated molecules in Schistosoma japonicum
- Parasitology (2008)

更多相關諮詢請洽

台灣代理廠商: 太鼎生物科技有限公司

業務代表: 許虹宜 0920312382 email: taiding.biotech@msa.hinet.net

公司電話: 02-86609496傳真: 02-86609342
公司網站: www.biopioneer.com.tw