太鼎生物科技/太鼎食安科技

AP抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#702-0010)

 Alkaline Phosphatase (AP) 抗體標誌directly labeling kit (貨號#702-0010)，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

AP酵素反應原理:

Alkaline phosphatase (AP) is a hydrolyase enzyme that is frequently conjugated to antibodies for use in immunoassays. PNPP is a popular colorimetric substrate for AP; the product of the reaction, p-nitrophenol, can be read at 405nM. Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate; MUP) may also be employed.

Lightning-link抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-Link®Alkaline Phosphatase (AP) 703-0010

Lightning-Link®Glucose Oxidase

Lightning-Link®Horseradish Peroxidase (HRP)

Lightning-Link®Avidin

Lightning-Link®Biotin

Lightning-Link®Streptavidin

Lightning-link酵素型抗體標誌---期刊發表:

Lightning-Link HRP ---期刊發表

• Measurement of MMP-9 and -12 degraded elastin (ELM) provides unique
  information on lung tissue degradation - BMC Pulmonary Medicine (2012)
• The carboxy-terminus of TFPIα is required for its interaction with factor V and Va - Journal of Thrombosis and Haemostasis (2012)
• Development of monoclonal antibody-based immunoassays for detection of Helicobacter pylori neutrophil-activating protein- Journal of Immunological Methods (2012)
• Sensitive quantitative assays for tau and phospho-tau in transgenic mouse models - Neurobiology of Aging (2012)
• A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants - Journal of Biomedicine and Biotechnology (2012)
• Porcine Reproductive and Respiratory Syndrome Virus Activates the Transcription of Interferon alpha/beta (IFN-α/β) in Monocyte-derived Dendritic Cells (Mo-DC) - Veterinary Microbiology (2012)
• A Human PrM Antibody That Recognizes a Novel Cryptic Epitope on Dengue E Glycoprotein - PLoS One (2012)
• A Conserved Tandem Cyclophilin-Binding Site in Hepatitis C Virus Nonstructural Protein 5A Regulates Alisporivir Susceptibility - Journal of Virology (2012)

• Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain - FEBS Journal (2011)
• Dual Neofunctionalization of a Rapidly Evolving Aquaporin-1 Paralog Resulted in Constrained and Relaxed Traits Controlling Channel Function during Meiosis Resumption in Teleosts - Mol Biol Evol (2011)
• Comparison of the efficiency of antibody selection from semi-synthetic scFv and non-immune Fab phage display libraries against protein targets for rapid development of diagnostic immunoassays- Journal of Immunological Methods (2011)
• Protein biomarkers in exfoliated cells collected from the human rectal mucosa: implications for colorectal disease detection and monitoring - International Journal of Colorectal Disease (2011)
• Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis - Fibrogenesis & Tissue Repair (2011)
• Canine specific ELISA for coagulation factor VII- The Veterinary Journal (2011)
• Aberrant splicing of the hRasGRP4 transcript and decreased levels of this signaling protein in the peripheral blood mononuclear cells in a subset of patients with rheumatoid arthritis- PLoS One (2011)
• MMP Mediated Degradation of Type VI Collagen Is Highly Associated with Liver Fibrosis – Identification and Validation of a Novel Biochemical Marker Assay - PLoS One (2011)
  
• Ubiquitin Fold Modifier 1 (UFM1) and Its Target UFBP1 Protect Pancreatic Beta Cells from ER Stress-Induced Apoptosis - PLoS One (2011)
• A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M) - Biomarkers (2011)
• Immunological detection of the type V collagen propeptide fragment, PVCP-1230, in connective tissue remodeling associated with liver fibrosis - Biomarkers (2011)
• Methodologic issues in the measurement of interleukin-16 in clinical blood samples using immunoassays - Cytokine (2011)
• Histidine-rich Glycoprotein Binds Fibrin(ogen) with High Affinity and Competes with Thrombin for Binding to the γ′-Chain- JBC (2011)

• Monoclonal antibody to novel cell surface epitope on Hsc70 promotes morphogenesis of bile ducts in newborn rat liver- Cell Stress and Chaperones (2010)
• Immunogenicity of attenuated measles virus engineered to express Helicobacter pylori neutrophil-activating protein- Vaccine (2010)
• Peptide Route Functionalization of ZnSe Crystals Preserves Activity and Structure of Proteins while Adsorption - The Journal of Physical Chemistry C (2010)
• Monoclonal antibody to novel cell surface epitope on Hsc70 promotes morphogenesis of bile ducts in newborn rat liver- Cell Stress and Chaperones (2010)
• Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells - JCB (2010)
• Regeneration of Dental-Pulp-like Tissue by Chemotaxis-Induced Cell Homing - Tissue Eng Part A. (2010)
• The Phosphatase Interactor NIPP1 Regulates the Occupancy of the Histone Methyltransferase EZH2 at Polycomb Targets – Nucleic Acids Research (2010)

• Extinction-Reconsolidation Boundaries: Key to Persistent Attenuation of Fear Memories - Science (2009)
• Development of a highly sensitive ELISA for aldosterone in mouse urine: Validation in physiological and pathophysiological states of aldosterone excess and depletion - Steroids (2009)
• Contributions to Neutropenia from PFAAP5 (N4BP2L2), a Novel Protein Mediating Transcriptional Repressor Cooperation between Gfi1 and Neutrophil Elastase - Molecular and Cellular Biology (2009)

LifeXtend HRP Conjugagte Stabilizer

• Sensitive quantitative assays for tau and phospho-tau in transgenic mouse models - Neurobiology of Aging (2012)

Lightning-Link Biotin ---期刊發表

• Controlled release of thymosin β4 from injected collagen–chitosan hydrogels promotes angiogenesis and prevents tissue loss after myocardial infarction - Regenerative Medicine (2012)
• Engrailed-2 (EN2): A Tumor Specific Urinary Biomarker for the Early Diagnosis of Prostate Cancer– Clinical Cancer Research (2011)
• Human Alpha Defensin 5 Expression in the Human Kidney and Urinary Tract - PLoS One (2011)
• Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract - Kidney International (2011)

• Method for Decreasing Immunogenicity ; Patent publication number US2011/0152505 (2010)
• Targeting MHC Class I Monomers to Dendritic Cells Inhibits the Indirect Pathway of Allorecognition and the Production of IgG Alloantibodies Leading to Long-Term Allograft Survival - Journal of Immunology (2010)
• Stromal Cell-Derived Factor-1α Activation of Tissue-Engineered Endothelial Progenitor Cell Matrix Enhances Ventricular Function After Myocardial Infarction by Inducing Neovasculogenesis - Cell Transplantation and Tissue Regeneration (2010)
• In Vivo Studies of Mutant Fibrillin-1 Microfibrils - JBC (2010)
• Serum insulin-like growth factor binding protein-1 (IGFBP-1) phosphorylation status in subjects with and without ischaemic heart disease - Artherosclerosis (2010)
• HER2 Phosphorylation Is Maintained by a PKB Negative Feedback Loop in Response to Anti-HER2 Herceptin in Breast Cancer – PLoS Biology (2010)

• Human Mesenchymal Stem Cells Self-Renew and Differentiate According to a Deterministic Hierarchy – PLoS One (2009)
• Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1- Biochemie (2009)

• Functional studies of host-specific ephrin-B ligands as Henipavirus receptors - Virology (2008)

Lightning-Link Avidin ---期刊發表:

• Targeting MHC Class I Monomers to Dendritic Cells Inhibits the Indirect Pathway of Allorecognition and the Production of IgG Alloantibodies Leading to Long-Term Allograft Survival – Journal of Immunology (2010)

Lightning-Link AP ---期刊發表

• Large molecular size EDTA-resistant complexes containing S100A12, ERAC, in serum during inflammatory conditions- Scandinavian Journal of Clinical & Laboratory Investigation (2012)
• Broad-Spectrum Inhibition of HIV-1 by a Monoclonal Antibody Directed against a gp120-Induced Epitope of CD4 - PLoS One (2011)
• Fluorescent Labeling of Antibody Fragments Using Split GFP - PLoS One (2011)
• In Vivo Studies of Mutant Fibrillin-1 Microfibrils - JBC (2010)
• Microarray immunoassay for the detection of grapevine and tree fruit viruses - Journal of Virological Methods (2009)