太鼎生物科技/太鼎食安科技

DESCRIPTION

Clone LT40

Ig Isotype Mouse IgM

Specificity Chicken CD45 (Mr 190 to 215-kDa).

Chicken CD45 is a transmembrane glycoprotein expressed on all
leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa
isoform while T lineage cells bear the 190 kDa variant. These high molecular
weight molecules have intrinsic phosphotyrosine phosphatase activity
characteristic of mammalian CD45.1 Levels of CD45 expression detected by MAb LT 40 in the avian thymus are heterogeneous with
approximately 90% of thymocytes expressing fourfold higher levels of surface
CD45 (CD45hi) than do the
remaining 10% (CD45lo) of thymocytes. The CD45lo population contains exclusively thymocytes with the CD3-CD4-CD8lo phenotype
characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population
which are CD45hi. This shift from low
to high levels of surface CD45 expression is concomitant with the transition
from CD4-CD8lo to CD4+CD8+ and before the
expression of CD3. The ability of MAb LT40 to discriminate this CD45lo subpopulation provides a suitable marker for the
CD3-CD4-CD8lo immediate precursors
to the CD3-CD4+CD8+ thymocytes.1