奈米金抗體標誌(InnovaCoat nanogold labeling ), 應用於lateral flow test快篩試驗的開發, 其靈敏度高於ELISA方式。
市面上, 奈米金抗體標誌產品, 受限於等電點及pH等問題, 在lateral flow test開發過程中, 奈米金標誌效能都不高, 而且還會有失效的問題,
InnovaCoat專利研發奈米金標誌, 其效能近乎100%, 且不須考量等電點及pH之問題,其特點如下所述:
- 抗體標誌效能近乎100%, 節省抗體用量。每次反應僅需要0.4ug~0.17ug抗體量 (濃度50ug/1000ul ~ 200ug/1000ul)。
- 一次反應, 可進行50次的lateral flow test。(體積為50ul, 可以稀釋為1ml)
- 一組共有10個反應, (500次, 17,500元, 平均一次約360元)。
- 抗體標誌完奈米金後, 其訊號高於傳統奈米金標誌。
- 不須考量等電點及pH問題。
- 奈米標誌僅需要15分鐘。
- 建立良好的奈金標誌抗體, 其靈敏度遠高於ELISA方法
- 傳統市售奈米金標誌, 其O.D為0.5, InnovaCoat的光密度為O.D=20
- 奈米金辨視NH3端, 以共價鍵鍵結, 性質穩定。
Gold Conjugation Kits
InnovaCoat™
A Revolution in Nano-Gold Conjugation
InnovaCoat™ GOLD products are ‘conjugation friendly’ nanoparticles with a proprietary surface coat that greatly enhances colloidal stability and permits easy covalent attachment of a variety of molecules, including antibodies, analytes and other biomolecules.
In the case of immuno-gold conjugates, the antibody can be attached irreversibly without the need for extensive trials at different values of pH and/or salt concentration, as is typical of traditional ‘passive’ binding methods.
In the case of analyte-gold conjugates, the multipoint attachment of the polymer to the gold surface ensures that the analyte is firmly anchored. By contrast, direct attachment of analytes to gold surfaces by self-assembly techniques is far less stable and unwanted desorption is a significant problem.
InnovaCoat™ GOLD nanoparticles can be provided in a variety of formats (e.g. 10 OD liquid or as lyophilized nanoparticles) formulated to allow a one-step reaction with antibodies.
The kits available are designed for research use and for the development of diagnostic products, specifically for the screening of potential antibodies or small scale ‘proof of principle’ studies. Please contact us if you require any additional information and to discuss your bulk or custom conjugation requirements.
- Proprietary surface coat
- Ultra-stable
- Covalently link antibodies and analytes
- Simple conjugation processes
- Different surface chemistries
- Custom labeling service available
- Antibody-GOLD conjugates in just 15 minutes
- Enhanced assay sensitivity
- Protective coat prevents metal-antibody interactions
- Rapid screening of multiple antibodies for assay development
- No pH titrations
- Fully scalable - from R&D to Production
- Custom InnovaCoat™ labeling services
Click images to view full screen:
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Figure 1.
A comparison between the percentage binding of anti-cortisol antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.
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Figure 2.
A comparison between the signal intensity, measured on a commercially available lateral flow strip reader, for anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.
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Figure 3.
A comparison between the percentage binding of anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.
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What can I do if my antibody preparation contains interfering substances?
The simplest procedure is to dialyse the antibody against a suitable buffer. Relatively weak buffers (e.g. 20mM) are preferred so that the pH conditions of the covalent reaction are not significantly altered upon addition of the antibody. See also the comments in Section 3 of the protocol. If dialysis is not practical you can use other popular methods of buffer exchange if required e.g. desalting columns.
Does the antibody bind to the metal surface?
No. The protective surface coat completely shields the metal surface and prevents direct metal-antibody interactions. For this reason, you cannot use InnovaCoat GOLD for passive conjugation of antibodies.
Can antibodies from different species be used?
Yes, the InnovaCoat system has been tested with antibodies from a variety of species including mouse, rabbit, goat and sheep.
What type of linkage to gold is formed?
The antibody becomes covalently and irreversibly attached via lysine residues to the InnovaCoat surface.
Can antibody fragments be conjugated?
Yes. One of the advantages of the protective coat is that it is less likely than a bare metal surface to cause denaturation and loss of affinity of the antibody fragments.
What if I need bulk material?
The kit that you have purchased is a convenience product for rapid production of small quantities of conjugate for screening purposes. Bulk quantities of InnovaCoat GOLD can be provided in a variety of formats. Please enquire.
Is the kit suitable for conjugating analytes and other small molecules?
Yes, but please contact Innova technical services. Depending on the functional groups on your analyte you may need a different type of surface or different chemistry for optimal results.
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