太鼎生物科技/太鼎食安科技

Description

Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni²⁺ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.

Applications

  Purification of His-tagged proteins.

  Antibody purification.

  Assays involving protein-protein and protein-DNA interactions.

  Structural and functional investigation of proteins.

Special Features

  One-step easy purification protocol from crude lysate to >95% pure protein.

  Purification under native or denaturing conditions.

  Ready-to-use for any scale of purification.

  Protein binding capacity: approx. 20-40 μmol Ni²⁺/ml gel.

  Economical value.

  Can be used for successive cycles.

  Resins and columns formats suitable for batch and gravity purification.

Related Products

Purified protein fractions can be detected using Anti-HIS-tag Antibody