Angioreactor改善Plug assay所造成的問題。毎管體積20ul, 一隻mice可移植4管。太鼎生物科技有限公司(02)86609496－太鼎生物科技/太鼎食安科技

活體血管新生套組產品介紹 Directed In Vivo Angiogenesis Assay

1. 活體血管新生分析，市上唯一可定量血管新生的套組。

2. 改善plus assay所造成的自體吸收問題。

3. 20ul體積, 每隻mice可移植4管, 實驗再現性高。

DIVAA™ Background/Significance

Invented and developed at the National Institute of Health by William Stetler-Stevenson, MD, Ph.D.
Quantitative Assay to Assess Angiogenesis In Vivo
Alternative to Plug Assay

【血管新生分析------動物物種選擇】

Most data has been generated with athymic nude mice, but success has been reported in C57Bl/6 as well.
• Recommend 6 to 8 weeks old.
• Studies have been done with both males and females. Females are recommended because males may remove angioreactors from each other via fighting.

【血管新生分析------埋殖準備】

• Optimal implantation periods may vary depending on experimental design:

– angiogenic factors
– doses
– mice
• Genetic background
• Athymic nude mice (9-15 days)
• C57Bl/6 mice (15 days)
• Increasing implantation period generally improves signal, but it may also increase background.
• Placement of the angioreactors is also critical.

Preparation

DIVAA Angiorack^™ Cat# 3450-048-09

Available as a separate product, the autoclavable, reusable Angiorack is precision machined of Teflon^® to hold 48 sterile silicone angioreactors in the laminar flow hood while filling and preparing for implantation.

Features:	This kit includes pathogen Free BME which has been tested and cleared of a Battery of 31 pathogens and viruses including LDEV. Contact Trevigen for details.

Applications:	Quantitative in vivo angiogenesis analysis, for the screening of inhibitors.

Components:	Part #	Component	Size
	3450-048-01	Angioreactors	48 units
	3450-048-02	Growth Factor Reduced BME PathClear	6 x 200 µl
	3450-048-03	Wash Buffer	25 ml
	3450-048-05	CellSperse™	15 ml
	3450-048-06	200X FITC-Lectin	250 µg/50 µl
	3450-048-07	25X FITC-Lectin Diluent	400 µl
	3450-048-08	Heparin Solution	10 µl
	3450-048-B10	FGF-2(1800 ng)/VEGF(600 ng)	60 µl

	*Basement Membrane Extract (BME)

Storage:	Components are stored at -20°C and 4°C.

References:	Guedez L, Rivera AM, Salloum R, Miller ML, Diegmueller JJ, Bungay PM, Stetler-Stevenson WG: Quantitative Assessment of Angiogenic Response by the Directed In Vivo Angiogenesis Assay. AJP 2003, pp 1431-1439.