太鼎生物科技/太鼎食安科技

Antibody Purification Kits

AbSelect Antibody Purification System

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions. The AbSelect™ Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum.

The antibody to be purified or cleaned up ideally is in a volume of 100ul to 0.5ml. Up to 500µg of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.

The components of the AbSelect kit are fully compatible with the Lightning-Link™ antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels.

AbSelect Serum Antibody Purification System

Antibodies generated from ascites fluid and serum are often supplied as crude formulations. Both ascites fluid and serum contain many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect Serum system is a fast and simple method to purify antibodies from these types of media.

AbSelect Serum is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from ascites fluid and serum.  The method involves the capture of the antibody on the AbSelect Serum resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.

The components of the AbSelect Serum purification system are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.

AbSelect TCS Antibody Purification System

Antibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies.

AbSelect TCS is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from hybridoma supernatants.  The method involves the capture of the antibody on the AbSelect TCS resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.

The components of the AbSelect TCS are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.

Antibody Concentration and Cleanup Kit

Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.

The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.

Protein A Agarose