太鼎生物科技/太鼎食安科技

粒線體膜電位之測定JC-1介紹

Mitochondria 與Apoptosis

JC-1A mitochondrial dye

JC-1染劑5, 5', 6, 6' -tetrachloro-1, 1', 3, 3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) ，被發現較 Rh123 或 DiOC6 對粒線體膜電位，具有更高的靈敏度和解析分辨之能力。可應用於測量單一粒線體的Dym。 JC-1 的特性，當 Dym 小於 100 mV 時，會以 monomer 的形式存在於粒線體之內膜， 488 nm 激發下會放出大約 ~525 nm (FL-1H in Flow cytometry) 的綠色螢光；當 Dym 逐漸升高時，JC-1 會聚集在粒線體內膜，形成橘色的 J-aggregates (~590 nm, FL-2H)；反之，當膜電位下降時，JC-1 又會形成綠色螢光的 monomer 形式 (FL-1H)。因此，JC-1 可說是一個很好的 potential-dependent 染劑，這種 potential-dependent 的色彩轉變，使得我們更容易在流式細胞儀 (flow cytometry) 或共軛焦顯微鏡來對 mitochondrial Dym 做定性與定量的分析 。

資料來源: http://www.cbt.ntu.edu.tw/General/BioMed/Biomed2/Biomed2-10.htm

Product Name: JC-1

Alternate Name/Synonyms: 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzimidazolylcarbocyanine Iodide

Description: JC-1 is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 monomer is in equilibrium with so called J-aggregates, which are favored at higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (lem = 527 nm), while the J-aggregates have red fluorescence (lem = 590 nm). Therefore, it has been possible to use fluorescence ratioing technique to study mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy cells, the dye aggregates in the mitochondria and fluoresces red.

Peptide Sequence: N/A

Appearance: Brown red solid

Formulation: N/A

CAS Number: 3520-43-2

Molecular Formula: C₂₅H₂₇Cl₄IN₄

Molecular Weight: 652.23

Purity: >98% by HPLC

Solubility:DMSO and DMF

Storage Temp.: -20°C

Shipping Conditions: gel pack

Handling: Protect from light and moisture

SMILES: CCN1C2=CC(=C(C=C2[N+](=C1C=CC=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-]

InChi: InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

InChi Key: FYNNIUVBDKICAX-UHFFFAOYSA-M

PubChem CID: 5492929

MDL Number: MFCD00188475

USAGE: For Research Use Only! Not For Use in Humans.