SuperKine™ 蛋白凝膠快速染色液(Coomassie Blue)|貨號BMU105-EN (贈品30ml) 

贈品DAPI螢光封片劑.jpg

SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)

 

考馬斯藍(Coomassie blue)是一種常用的蛋白質可視化染料(通過蛋白質凝膠電泳分離, protein gel electrophoresis)。在酸性條件(acidic conditions)下,考馬斯藍與蛋白質的鹼性疏水性氨基酸殘基(hydrophobic amino acid residues)結合,呈深藍色。 Abbkine研發的即用型SuperKine™蛋白凝膠快速染色液(SuperKine™ Protein Gel Fast Staining Solution)(考馬斯藍)具有染色時間短(short dyeing time)、顯色清晰(clear color rendering)、重複性好(good repeatability)、安全性高等優點,無需脫色(without decolorization)即可獲得清晰乾淨的背景。基於經典的Coomassie blue染色原理,染色後回收的目標蛋白可用於後續的質譜實驗(mass spectrometry experiments)或測序分析(sequencing analysis)。

BMU105.jpg

 

圖1 使用SuperKine™蛋白凝膠快速染色液(考馬斯亮藍)對BSA進行染色,並與A、B廠家的染色液進行比較。上樣量分別為Marker、10 ng、20 ng、50 ng、100 ng、200 ng、500 ng、1000 ng、2000 ng、5000 ng、Marker。 Abbkine 的 SuperKine™ 蛋白凝膠快速染色液(考馬斯藍)無論染色 15 分鐘還是 1 小時,結果都更加清晰。

Product name SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)
Applications notes SDS-PAGE gel electrophoresis, Non-denaturing PAGE gel electrophoresis.
Alternative Protein staining; Gel staining
Formulation Liquid solution
Kit components SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)
Features & Benefits • Fast response: Mainly contains uniquely developed dyes/Dyeing can be done in as fast as 10 min.
• High security: Add unique non-toxic optimized ingredients/No need for decolorization, and no need for immobilization of methanol and ethanol.
• High sensitivity: Add signal-enhancing dye components/Can detect proteins as low as 10 ng.
• Easy to operate: Ready to use/No need to prepare, can be used directly.
Limit of detection 10 ng
Usage notes • Protein Gel Fast Staining Solution contains volatile substances, please seal it to ensure its performance.
• When using Protein Gel Fast Staining Solution, you need to prepare deionized water and Staining container before the experiments.
• The washing and Staining time mentioned in the experimental steps are applicable to the gel with a thickness of 0.75 mm to 1.5 mm. For the thicker gel, the dyeing time can be appropriately extended according to the color rendering effect of the target protein band.
• This Staining solution is acidic and slightly corrosive. Wear lab coat, gloves and other protective equipment when using.
• Residual impurities can increase the staining background and reduce target protein resolution, so it is necessary to wash thoroughly with deionized water before staining to remove impurities.
Storage instructions Stored at 4℃ for 12 months, protected from light.
Shipping Gel pack with blue ice.
Precautions The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes.

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

產品貨號 產品品項 反應 測量儀器
貨號KTA1001 活細胞和死細胞雙染色試劑盒 100個反應

活細胞綠色螢光染劑Ex/Em = 488/530 nm;

死細胞紅色螢光染料(Ex/Em = 535/617)

貨號 KTA2010

 

TUNEL細胞凋亡檢測試劑盒

 

50個反應 螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道(Ex / Em = 490nm / 520 nm)

貨號 KTC011001

 

CCK-8細胞增殖和細胞毒性試劑盒 500個反應 吸收光450nm
貨號KTC4003 TraKine™粒線體染色 50個反應/250個反應 Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100 Pro活細胞微管蛋白染色 50個反應/250個反應 Ex/Em = 500/520 nm
貨號KTC4001 Abbkine 細胞膜染色  100個反應/500個反應 Ex/Em = 484/501 nm
貨號BMD00063 DAPI Staining Solution 10MG/100MG Ex/Em: 358/461 nm (with DNA)
貨號KTC4210 活細胞Lysosome染色 50個反應/250個反應 deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030 細胞衰老檢測試劑盒(β-半乳糖苷酶) 100個反應 光學顯微鏡
貨號KTA1020 CCK-8細胞增殖和細胞毒性試劑盒 1000個反應/10,000個反應 吸收光450nm
貨號KTA2030

細胞增殖EdU Image試劑盒

(BrdU替代法)

100個反應/500個反應 AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400 一氧化Dan(NO)檢測試劑盒 

96個反應/

192個反應

colorimetric (540 nm)

檢測範圍:1-100 µM

貨號KTB1070 CheKine™黃嘌呤氧化酶檢測

96個反應/

480個反應

OD 450 nm

線性範圍15.6 -500 mU/

貨號KTD3001  BCA蛋白定量試劑盒

2000個反應/

5000個反應

OD 562 nm

相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80

50 to 1000 µg/ml.

貨號KTB1110 乳酸脫氫酶(LDH)測定試劑盒

96個反應/

480個反應

λmax= 450 nm

1- 20 U/mL of LDH

貨號PRP100153 IGF-I重組蛋白 200UG/1MG

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)

> 95 % as determined by SDS-PAGE

貨號 KTA2020 細胞週期染色檢測 100個反應/500個反應 Ex/Em=535/615 nm.
貨號BMC21400 PurKine™內毒素去除預裝離心柱

1ml×5

3mlx5

More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200 CheKine™蛋白羰基比色測定試劑盒 

48個反應/

96個反應

吸收光370 nm
貨號KTB1080 超氧陰離子清除測定試劑盒

48個反應/

96個反應

Superoxide anion scavenging rate D% =(∆∆ODControl - ∆∆ODSample)÷ ∆∆ODControl ×100%
貨號KTB1090 清除氫氧自由基能力測定試劑

48個反應/

96個反應

吸收光 536nm Hydroxyl radical scavenging rate D% =(ASample - AControl)÷(ABlank - AControl)×100%
貨號KTB2220 TC總膽固醇檢測試劑盒

48個反應/

96個反應

500nm Standard: 5 μmol/mL
貨號KTB1510 CheKine™尿酸(UA)比色分析試劑盒

48個反應/

96個反應

 吸收光505 nm

Standard: 5 μmol/mL.

貨號KTB1300 Glucose葡萄糖測定試劑盒

96個反應/

192個反應

 吸光值630nm

4- 300 mg/dL)

貨號KTB1100 Lactate  乳酸檢測試劑盒 

96個反應/

480個反應

colorimetric (450 nm)

0.03 mM-2 mM

貨號KTB1700 組織和血液鹼性磷酸酶( Alkaline Phosphatase, AKP / ALP)比色測定

96個反應/

96 X5盤

吸收光510 nm
貨號KTB1500 總抗氧化劑能力TAC分析

96個反應/

480個反應

593 nm

偵測範圍: 0.15-3 mM

貨號KTA0002 Annexin V-AbFluor™488細胞凋亡檢測試劑盒 20個反應/50個反應/100個反應 Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KET7013 EliKine™ Rat IL-6 ELISA Kit試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL

貨號KET0003 雌二醇(Estradiol , E2)試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍40 pg/ml-1500 pg/ml

貨號KET6032 TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒

96個反應/

96 X5盤

450 nm

7.8 pg/ml-500 pg/ml

貨號KET6033 VEGF血管內皮生長因子ELISA試劑盒 

96個反應/

96 X5盤

450 nm

偵測範圍: 31.25 pg/mL-2000 pg/mL

貨號KET7005 白介素-1β(Interleukin-1β, IL-1β)ELISA試劑盒

96個反應/

96 X5盤

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

偵測範圍: 15.6 pg/ml-1000 pg/ml

貨號KTE70003 小鼠黃嘌呤脫氫酶/氧化酶(XDH)ELISA試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。

貨號 A02051HRP His-tag標籤多株抗體-HRP  50ul/200ul WB (1:5000).
貨號A02170MGB Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠 100ul/400ul/2ml GKPIPNPLLGLDST epitope
貨號KTA4001 JC-1線粒體膜電位測定試劑盒

100個反應/

500個反應

(聚結的JC-1,Ex / Em = 585/590 nm)發出綠色螢光(JC-1單體,Ex / Em = 510/527 nm)

JC-1 Stain

CCCP (10 mM)  Assay Buffer (5×)

貨號KTB1600 穀胱甘肽(Glutathione)檢測 96個反應 412 nm  2-200 µg/mL
貨號BMD00063 DAPI Staining Solution 1MG/10MG/100MG λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001 細胞核/細胞質分離試劑盒

50個反應/

200個反應

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG Calcein AM鈣黃綠素活細胞螢光染劑 50ug/1mg/10mg

λEX/λEm: 494/517 nm (pH 8) (calcein)

 

貨號 KTB1660 硫氧還蛋白過氧化物酶(TPX)檢測  96個反應 240nm
貨號KTB1020 NAD/NADH 分析試劑盒

96個反應/

480個反應

OD565 nm

96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。

貨號KTB1070 CheKine™黃嘌呤氧化酶檢測

96個反應/

480個反應

OD 450 nm

線性範圍15.6 -500 mU/mL

貨號KTB1040 Catalase過氧化氫酶活性檢測試劑盒

96個反應/

480個反應

OD 540 nm

2-75 µM

貨號KTB1060 過氧化物酶(Peroxidases)分析試劑盒 96個反應

OD 664 nm

線性範圍為15.6-1000 µU/mL

貨號KTB1050 脂質過氧化檢測試劑盒(MDA) 96個反應

OD 532 nm

1-100 µM/L

貨號KTB1030 超氧化物歧化酶(SOD)活性測定試劑盒  96個反應 200-3.13 U/ml
貨號KTA1030 LDH細胞毒性測定試劑盒

96個反應/

480個反應

 OD 565 nm

10,00-100,0000 cells per well

貨號KTB3010 CheKine™ 花青素還原酶(ANR)活性比色測定試劑盒

48個反應/

96個反應

absorption at 340 nm
貨號KTB1542 Tannase(TAN) 活性比色測定試劑盒 

48個反應/

96個反應

absorbance at 270 nm

0.3125-20 μmoL/mL

貨號KTB1541 CheKine™ 單寧比色測定試劑盒 | CheKine™ Tannin Colorimetric Assay Kit

48個反應/

96個反應

absorbance at 760 nm

0.0156-1 mg/mL

貨號KTB2250 CheKine™ 微量高密度脂蛋白膽固醇 (HDL-C) 檢測試劑盒

48個反應/

96個反應

Absorption peak at 500nm

Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol/L)

貨號KTB2260 CheKine™ 微量低密度脂蛋白膽固醇 (LDL-C) 檢測試劑盒

48個反應/

96個反應

Absorption peak at 500nm

Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol/L)

貨號KTB1290 CheKine™ 烏頭酸酶 (ACO) 活性比色測定試劑盒

48個反應/

96個反應

absorption at 340 nm.
貨號KTB1560 醇醯基轉移酶(AAT)活性比色檢測試劑盒 

48個反應/

96個反應

absorption at 412 nm.
貨號KTB1390 CheKine™ 澱粉分支酶 (SBE) 活性比色測定試劑盒

48個反應/

96個反應

absorption at 660 nm
貨號PRP1012 Human IL-6 protein重組蛋白  5ug/20ug/100ug/1mg human IL-6 (NP_000591.1) (Val 30-Met 212)/ E. coli
貨號PRP100273 Human IL-4 protein重組蛋白 20ug/100ug/1mg ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg.
貨號PRP100159 Human EGF protein重組蛋白 100ug/1mg  
貨號KTB1041 CheKine™ 過氧化氫 (H2O2) 檢測試劑盒

96個反應/

480個反應

OD 580 nm

: 1 µM-100 µM

貨號KTB1210 CheKine™ 超氧陰離子比色測定試劑盒

48個反應/

96個反應

absorption peak at 540nm

0.0125-0.5 mg/mL

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