SuperKine™ 蛋白凝膠快速染色液（Coomassie Blue）|貨號BMU105-EN (贈品30ml)
SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)
考馬斯藍(Coomassie blue)是一種常用的蛋白質可視化染料（通過蛋白質凝膠電泳分離, protein gel electrophoresis）。在酸性條件(acidic conditions)下，考馬斯藍與蛋白質的鹼性疏水性氨基酸殘基(hydrophobic amino acid residues)結合，呈深藍色。 Abbkine研發的即用型SuperKine™蛋白凝膠快速染色液(SuperKine™ Protein Gel Fast Staining Solution)（考馬斯藍）具有染色時間短(short dyeing time)、顯色清晰(clear color rendering)、重複性好(good repeatability)、安全性高等優點，無需脫色(without decolorization)即可獲得清晰乾淨的背景。基於經典的Coomassie blue染色原理，染色後回收的目標蛋白可用於後續的質譜實驗(mass spectrometry experiments)或測序分析(sequencing analysis)。
圖1 使用SuperKine™蛋白凝膠快速染色液（考馬斯亮藍）對BSA進行染色，並與A、B廠家的染色液進行比較。上樣量分別為Marker、10 ng、20 ng、50 ng、100 ng、200 ng、500 ng、1000 ng、2000 ng、5000 ng、Marker。 Abbkine 的 SuperKine™ 蛋白凝膠快速染色液（考馬斯藍）無論染色 15 分鐘還是 1 小時，結果都更加清晰。
| Product name | SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) |
| Applications notes | SDS-PAGE gel electrophoresis, Non-denaturing PAGE gel electrophoresis. |
| Alternative | Protein staining; Gel staining |
| Formulation | Liquid solution |
| Kit components | SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) |
| Features & Benefits | • Fast response: Mainly contains uniquely developed dyes/Dyeing can be done in as fast as 10 min. • High security: Add unique non-toxic optimized ingredients/No need for decolorization, and no need for immobilization of methanol and ethanol. • High sensitivity: Add signal-enhancing dye components/Can detect proteins as low as 10 ng. • Easy to operate: Ready to use/No need to prepare, can be used directly. |
| Limit of detection | 10 ng |
| Usage notes | • Protein Gel Fast Staining Solution contains volatile substances, please seal it to ensure its performance. • When using Protein Gel Fast Staining Solution, you need to prepare deionized water and Staining container before the experiments. • The washing and Staining time mentioned in the experimental steps are applicable to the gel with a thickness of 0.75 mm to 1.5 mm. For the thicker gel, the dyeing time can be appropriately extended according to the color rendering effect of the target protein band. • This Staining solution is acidic and slightly corrosive. Wear lab coat, gloves and other protective equipment when using. • Residual impurities can increase the staining background and reduce target protein resolution, so it is necessary to wash thoroughly with deionized water before staining to remove impurities. |
| Storage instructions | Stored at 4℃ for 12 months, protected from light. |
| Shipping | Gel pack with blue ice. |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
| 產品貨號 | 產品品項 | 反應 | 測量儀器 |
| 貨號KTA1001 | 活細胞和死細胞雙染色試劑盒 | 100個反應 |
活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617) |
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50個反應 | 螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道（Ex / Em = 490nm / 520 nm） |
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CCK-8細胞增殖和細胞毒性試劑盒 | 500個反應 | 吸收光450nm |
| 貨號KTC4003 | TraKine™粒線體染色 | 50個反應/250個反應 | Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 50個反應/250個反應 | Ex/Em = 500/520 nm |
| 貨號KTC4001 | Abbkine 細胞膜染色 | 100個反應/500個反應 | Ex/Em = 484/501 nm |
| 貨號BMD00063 | DAPI Staining Solution | 10MG/100MG | Ex/Em: 358/461 nm (with DNA) |
| 貨號KTC4210 | 活細胞Lysosome染色 | 50個反應/250個反應 | deep red fluorescent dye , Ex/Em:650/665nm |
| 貨號KTA3030 | 細胞衰老檢測試劑盒（β-半乳糖苷酶） | 100個反應 | 光學顯微鏡 |
| 貨號KTA1020 | CCK-8細胞增殖和細胞毒性試劑盒 | 1000個反應/10,000個反應 | 吸收光450nm |
| 貨號KTA2030 |
(BrdU替代法) |
100個反應/500個反應 | AbFluor 488 azide (Ex/Em = 501/525 nm) |
| 貨號KTB1400 | 一氧化Dan（NO）檢測試劑盒 |
96個反應/ 192個反應 |
colorimetric (540 nm) 檢測範圍：1-100 µM |
| 貨號KTB1070 | CheKine™黃嘌呤氧化酶檢測 |
96個反應/ 480個反應 |
OD 450 nm 線性範圍15.6 -500 mU/ |
| 貨號KTD3001 | BCA蛋白定量試劑盒 |
2000個反應/ 5000個反應 |
OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml. |
| 貨號KTB1110 | 乳酸脫氫酶（LDH）測定試劑盒 |
96個反應/ 480個反應 |
λmax= 450 nm 1- 20 U/mL of LDH |
| 貨號PRP100153 | IGF-I重組蛋白 | 200UG/1MG |
human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE |
| 貨號 KTA2020 | 細胞週期染色檢測 | 100個反應/500個反應 | Ex/Em=535/615 nm. |
| 貨號BMC21400 | PurKine™內毒素去除預裝離心柱 |
1ml×5 3mlx5 |
More than 2,000,000 EU/mL to eliminate >99% of endotoxins. |
| 貨號KTA1200 | CheKine™蛋白羰基比色測定試劑盒 |
48個反應/ 96個反應 |
吸收光370 nm |
| 貨號KTB1080 | 超氧陰離子清除測定試劑盒 |
48個反應/ 96個反應 |
Superoxide anion scavenging rate D% =（∆∆ODControl - ∆∆ODSample）÷ ∆∆ODControl ×100% |
| 貨號KTB1090 | 清除氫氧自由基能力測定試劑 |
48個反應/ 96個反應 |
吸收光 536nm Hydroxyl radical scavenging rate D% =（ASample - AControl）÷（ABlank - AControl）×100% |
| 貨號KTB2220 | TC總膽固醇檢測試劑盒 |
48個反應/ 96個反應 |
500nm Standard: 5 μmol/mL |
| 貨號KTB1510 | CheKine™尿酸（UA）比色分析試劑盒 |
48個反應/ 96個反應 |
吸收光505 nm Standard: 5 μmol/mL. |
| 貨號KTB1300 | Glucose葡萄糖測定試劑盒 |
96個反應/ 192個反應 |
吸光值630nm 4- 300 mg/dL) |
| 貨號KTB1100 | Lactate 乳酸檢測試劑盒 |
96個反應/ 480個反應 |
colorimetric (450 nm) 0.03 mM-2 mM |
| 貨號KTB1700 | 組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定 |
96個反應/ 96 X5盤 |
吸收光510 nm |
| 貨號KTB1500 | 總抗氧化劑能力TAC分析 |
96個反應/ 480個反應 |
593 nm 偵測範圍: 0.15-3 mM |
| 貨號KTA0002 | Annexin V-AbFluor™488細胞凋亡檢測試劑盒 | 20個反應/50個反應/100個反應 | Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA) |
| 貨號KET7013 | EliKine™ Rat IL-6 ELISA Kit試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL |
| 貨號KET0003 | 雌二醇（Estradiol , E2）試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍40 pg/ml-1500 pg/ml |
| 貨號KET6032 | TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 7.8 pg/ml-500 pg/ml |
| 貨號KET6033 | VEGF血管內皮生長因子ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL |
| 貨號KET7005 | 白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒 |
96個反應/ 96 X5盤 |
Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml |
| 貨號KTE70003 | 小鼠黃嘌呤脫氫酶/氧化酶（XDH）ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。 |
| 貨號 A02051HRP | His-tag標籤多株抗體-HRP | 50ul/200ul | WB (1:5000). |
| 貨號A02170MGB | Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠 | 100ul/400ul/2ml | GKPIPNPLLGLDST epitope |
| 貨號KTA4001 | JC-1線粒體膜電位測定試劑盒 |
100個反應/ 500個反應 |
（聚結的JC-1，Ex / Em = 585/590 nm）發出綠色螢光（JC-1單體，Ex / Em = 510/527 nm） JC-1 Stain CCCP (10 mM) Assay Buffer (5×) |
| 貨號KTB1600 | 穀胱甘肽(Glutathione)檢測 | 96個反應 | 412 nm 2-200 µg/mL |
| 貨號BMD00063 | DAPI Staining Solution | 1MG/10MG/100MG | λEx/λEm: 358/461 nm (with DNA) |
| 貨號KTP3001 | 細胞核/細胞質分離試劑盒 |
50個反應/ 200個反應 |
Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X) |
| 貨號BMD00064-50UG | Calcein AM鈣黃綠素活細胞螢光染劑 | 50ug/1mg/10mg |
λEX/λEm: 494/517 nm (pH 8) (calcein)
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| 貨號 KTB1660 | 硫氧還蛋白過氧化物酶（TPX）檢測 | 96個反應 | 240nm |
| 貨號KTB1020 | NAD/NADH 分析試劑盒 |
96個反應/ 480個反應 |
OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。 |
| 貨號KTB1070 | CheKine™黃嘌呤氧化酶檢測 |
96個反應/ 480個反應 |
OD 450 nm 線性範圍15.6 -500 mU/mL |
| 貨號KTB1040 | Catalase過氧化氫酶活性檢測試劑盒 |
96個反應/ 480個反應 |
OD 540 nm 2-75 µM |
| 貨號KTB1060 | 過氧化物酶（Peroxidases）分析試劑盒 | 96個反應 |
OD 664 nm 線性範圍為15.6-1000 µU/mL |
| 貨號KTB1050 | 脂質過氧化檢測試劑盒(MDA) | 96個反應 |
OD 532 nm 1-100 µM/L |
| 貨號KTB1030 | 超氧化物歧化酶（SOD）活性測定試劑盒 | 96個反應 | 200-3.13 U/ml |
| 貨號KTA1030 | LDH細胞毒性測定試劑盒 |
96個反應/ 480個反應 |
OD 565 nm 10,00-100,0000 cells per well |
| 貨號KTB3010 | CheKine™ 花青素還原酶（ANR）活性比色測定試劑盒 |
48個反應/ 96個反應 |
absorption at 340 nm |
| 貨號KTB1542 | Tannase(TAN) 活性比色測定試劑盒 |
48個反應/ 96個反應 |
absorbance at 270 nm 0.3125-20 μmoL/mL |
| 貨號KTB1541 | CheKine™ 單寧比色測定試劑盒 | CheKine™ Tannin Colorimetric Assay Kit |
48個反應/ 96個反應 |
absorbance at 760 nm 0.0156-1 mg/mL |
| 貨號KTB2250 | CheKine™ 微量高密度脂蛋白膽固醇 (HDL-C) 檢測試劑盒 |
48個反應/ 96個反應 |
Absorption peak at 500nm Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol/L） |
| 貨號KTB2260 | CheKine™ 微量低密度脂蛋白膽固醇 (LDL-C) 檢測試劑盒 |
48個反應/ 96個反應 |
Absorption peak at 500nm Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol/L） |
| 貨號KTB1290 | CheKine™ 烏頭酸酶 (ACO) 活性比色測定試劑盒 |
48個反應/ 96個反應 |
absorption at 340 nm. |
| 貨號KTB1560 | 醇醯基轉移酶（AAT）活性比色檢測試劑盒 |
48個反應/ 96個反應 |
absorption at 412 nm. |
| 貨號KTB1390 | CheKine™ 澱粉分支酶 (SBE) 活性比色測定試劑盒 |
48個反應/ 96個反應 |
absorption at 660 nm |
| 貨號PRP1012 | Human IL-6 protein重組蛋白 | 5ug/20ug/100ug/1mg | human IL-6 (NP_000591.1) (Val 30-Met 212)/ E. coli |
| 貨號PRP100273 | Human IL-4 protein重組蛋白 | 20ug/100ug/1mg | ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg. |
| 貨號PRP100159 | Human EGF protein重組蛋白 | 100ug/1mg | |
| 貨號KTB1041 | CheKine™ 過氧化氫 (H2O2) 檢測試劑盒 |
96個反應/ 480個反應 |
OD 580 nm : 1 µM-100 µM |
| 貨號KTB1210 | CheKine™ 超氧陰離子比色測定試劑盒 |
48個反應/ 96個反應 |
absorption peak at 540nm 0.0125-0.5 mg/mL |
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