人褪黑激素 (Melatonin ,MT) ELISA 試劑盒 | 貨號KTE61518

產品描述:人褪黑激素 (Melatonin ,MT) ELISA 試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的人褪黑激素 (Melatonin ,MT) ELISA 試劑盒。對人褪黑激素 (Melatonin ,MT)特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中,並且存在的任何人褪黑激素 (Melatonin ,MT)都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合人褪黑激素檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的人褪黑激素 (Melatonin ,MT) ELISA 試劑盒的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 3.9 pg/mL-250 pg/mL, 最低靈敏度2 pg/ml可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum。

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Product name Human Melatonin (MT) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes Human Melatonin (MT) ELISA Kit employs a two-site sandwich ELISA to quantitate Melatonin in samples. An antibody specific for Melatonin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Melatonin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Melatonin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Melatonin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA
Calibration range 2 ng/L-32 ng/L
Limit of detection 0.1 ng/L

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