人白介素IL-1β(Interleukin 1β)試劑盒 | EliKine™ Human IL-1β ELISA Kit Abbkine貨號KET6013－太鼎生物科技/太鼎食安科技

人白介素IL-1β(Interleukin 1β)試劑盒 | EliKine™ Human IL-1β ELISA Kit 貨號KET6013

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細胞因子(Cytokine)功能

細胞因子(Cytokine)具有多種生物學功能。它是急性期反應(acute phase response)的有效誘導劑。在B細胞(B-cells)最終分化為Ig分泌細胞(Ig-secreting cells)的最終分化中起重要作用，參與淋巴細胞(lymphocyte)和單核細胞(monocyte)的分化。它誘導骨髓瘤(myeloma)和漿細胞瘤(plasmacytoma growth)生長，並誘導神經細胞分化(differentiation)。作用於B細胞(B-cells)，T細胞(T-cells)，肝細胞(hepatocytes)，造血祖細胞(hematopoeitic progenitor cells)和CNS中樞神經系統細胞。也可以作為肌肉因子(myokine)。它在肌肉收縮後排入血液，並起到增加脂肪分解和改善胰島素抵抗(insulin resistance)的作用。

炎性細胞因子

炎症由多種可溶性因子介導，包括一組稱為細胞因子的分泌多肽。炎性細胞因子可分為兩類：參與急性炎症的細胞因子和負責任的細胞因子。炎症是組織對損傷的反應，其特徵是在急性期以增加的血流量和血管通透性以及體液，白細胞和炎性介質（例如細胞因子）的積累為特徵。幾種細胞因子在介導急性炎症反應中起關鍵作用，即IL-1，TNF-α，IL-6，IL-11，IL-8和其他趨化因子，G-CSF和GM-CSF。

已知可介導慢性炎症過程的細胞因子可分為參與體液炎症的細胞因子，例如IL-3，IL-4，IL-5，IL-6，IL-7，IL-9，IL-10，IL- 13，轉化生長因子b（TGF-b），以及那些有助於細胞炎症的因子，例如IL-1，IL-2，IL-3，IL-4，IL-7，IL-9，IL-10， IL-12，干擾素（IFN），IFN-g誘導因子（IGIF），TGF-b和TNF-a和-b。炎症異常是一大類疾病，是許多人類疾病的基礎。細胞因子是宿主對感染，免疫反應，炎症和創傷反應的調節劑。一些細胞因子起使疾病惡化的作用（促炎細胞因子），而另一些細胞因子起減輕炎症和促進癒合的作用（抗炎細胞因子）。

白介素-1（Interleukin-1, IL-1）是有史以來最早的細胞因子(cytokines)之一。它的最初發現是可能誘發發燒(fever)，控制淋巴細胞(lymphocytes)，增加骨髓細胞數量(bone marrow cells)並引起骨關節退化的因素。此時，IL-1以其他幾種名稱為人所知，包括內源性熱原(endogenous pyrogen)，淋巴細胞活化因子(lymphocyte activating factor)，造血素-1(haemopoetin-1)和單核細胞因子(mononuclear cell factor)等。大約在1984年至1985年間，科學家們證實IL-1實際上由兩種不同的蛋白質組成，現在稱為IL-1α和IL-1β，它們屬於稱為白細胞介素1超家族的細胞因子家族。 IL-1β由巨噬細胞(macrophages)，單核細胞(monocytes)和樹突狀細胞(dendritic cells)產生。

IFN-γ（IFN-gamma, 干擾素-γ）是促炎(proinflammatory)細胞因子(cytokine)的原型(prototype)，由多種免疫細胞(immune cells)在炎性(inflammatory)條件下產生，特別是由T細胞(T cells)和NK細胞(NK cells)產生。它通過促進Th1細胞的發育(development)和激活(activation)，單核細胞(monocytes)和巨噬細胞(macrophage)的化學引誘(chemoattraction)和激活(activation)，抗原呈遞分子(antigen presentation molecules)的上調以及B細胞中的免疫球蛋白類別轉換(immunoglobulin class switching)，在宿主防禦中起關鍵作用。它還具有抗病毒(antiviral)，抗增殖(antiproliferative)和凋亡作用(apoptotic)。另外，IFN-γ通過促進調節性T細胞的發育並抑制Th17細胞分化而充當抗炎介質。 IFN-γ二聚體通過兩個IFN-γR1和兩個IFN-γR2亞基的受體複合物發出信號。

產品描述：EliKine™人白介素IL-1β(Interleukin 1β)試劑盒採用兩點夾心ELISA定量樣品中的白介素IL-1β(Interleukin 1β)。對白介素IL-1β(Interleukin 1β)特異的抗體已預先包被在微板上。將標準品和樣品移入孔中，並且所存在的任何白介素IL-1β(Interleukin 1β)都與固定的抗體結合。除去任何未結合的物質後，將對白介素IL-1β(Interleukin 1β)特異的生物素偶聯抗體添加到孔中。洗滌後，將專有的EliKine™鏈黴親和素-HRP偶聯物添加到孔中。洗滌以除去任何未結合的鏈黴親和素酶試劑後，將底物溶液添加至孔中，並且顏色與初始步驟中結合的白介素IL-1β(Interleukin 1β) 的數量成比例。停止顯色並測量顏色強度。偵測範圍: 7.8 pg/ml-500 pg/ml, 最低靈敏度4 pg/mL。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

Interleukin 1β encoded by IL-1β gene is a member of the interleukin 1 cytokine family. Interleukin 1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). Interleukin 1β is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by Interleukin 1β in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL-1β gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Product name

EliKine™ Human IL-1β ELISA Kit

Reactivity

Human

Applications

ELISA

Application notes

EliKine™ Human IL-1β ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1β in samples. An antibody specific for IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1β present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1β is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection method

Colorimetric

Sample type

Cell culture supernatants, Other biological fluids, Plasma, Serum

Assay type

Sandwich ELISA (quantitative)

Assay duration

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit components
Human IL-1β microplate
• Human IL-1β standard
• Human IL-1β detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits

EliKine™ Human IL-1β ELISA Kit has high sensitivity and excellent specificity for detection of Human IL-1β. No significant cross-reactivity or interference between Human IL-1β and analogues was observed.